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A Two-Stage Enzymolysis Method and Its Application in Exerting Antioxidant Activity of Walnut Protein
Traditional enzymolysis method for producing bioactive peptides does not consider the utilization of digestive enzymes in the human gastrointestinal tract, leading to the possibility of excessive hydrolysis and higher production cost. Therefore, a two-stage enzymolysis method was established in this...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046995/ https://www.ncbi.nlm.nih.gov/pubmed/35495928 http://dx.doi.org/10.3389/fnut.2022.889434 |
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author | Liu, Dandan Chen, Min Zhu, Junsong Tian, Weijie Guo, Yiting Ma, Haile |
author_facet | Liu, Dandan Chen, Min Zhu, Junsong Tian, Weijie Guo, Yiting Ma, Haile |
author_sort | Liu, Dandan |
collection | PubMed |
description | Traditional enzymolysis method for producing bioactive peptides does not consider the utilization of digestive enzymes in the human gastrointestinal tract, leading to the possibility of excessive hydrolysis and higher production cost. Therefore, a two-stage enzymolysis method was established in this study based on in vitro limited enzymolysis and gastrointestinal digestion, and applied it to the research of walnut protein (WP) in exerting antioxidant activity. Results showed that WP could be well-digested by pepsin and pancreatin. WP with limited enzymolysis degree of 0% could achieve high antioxidant activity after the simulated gastrointestinal digestion, and the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and reducing power were 66.53% and 8.55 μmoL TE/mL, respectively. In vivo experimental results also exhibited that both WP and WP hydrolysate (WPH) could alleviate the oxidative damage induced by D-galactose in SD rats to some extent. Considering the digestive function of human body, in vitro limited enzymolysis, in vitro simulated gastrointestinal digestion and in vivo validation are necessary processes for the production of bioactive peptides. |
format | Online Article Text |
id | pubmed-9046995 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90469952022-04-29 A Two-Stage Enzymolysis Method and Its Application in Exerting Antioxidant Activity of Walnut Protein Liu, Dandan Chen, Min Zhu, Junsong Tian, Weijie Guo, Yiting Ma, Haile Front Nutr Nutrition Traditional enzymolysis method for producing bioactive peptides does not consider the utilization of digestive enzymes in the human gastrointestinal tract, leading to the possibility of excessive hydrolysis and higher production cost. Therefore, a two-stage enzymolysis method was established in this study based on in vitro limited enzymolysis and gastrointestinal digestion, and applied it to the research of walnut protein (WP) in exerting antioxidant activity. Results showed that WP could be well-digested by pepsin and pancreatin. WP with limited enzymolysis degree of 0% could achieve high antioxidant activity after the simulated gastrointestinal digestion, and the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and reducing power were 66.53% and 8.55 μmoL TE/mL, respectively. In vivo experimental results also exhibited that both WP and WP hydrolysate (WPH) could alleviate the oxidative damage induced by D-galactose in SD rats to some extent. Considering the digestive function of human body, in vitro limited enzymolysis, in vitro simulated gastrointestinal digestion and in vivo validation are necessary processes for the production of bioactive peptides. Frontiers Media S.A. 2022-04-14 /pmc/articles/PMC9046995/ /pubmed/35495928 http://dx.doi.org/10.3389/fnut.2022.889434 Text en Copyright © 2022 Liu, Chen, Zhu, Tian, Guo and Ma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Nutrition Liu, Dandan Chen, Min Zhu, Junsong Tian, Weijie Guo, Yiting Ma, Haile A Two-Stage Enzymolysis Method and Its Application in Exerting Antioxidant Activity of Walnut Protein |
title | A Two-Stage Enzymolysis Method and Its Application in Exerting Antioxidant Activity of Walnut Protein |
title_full | A Two-Stage Enzymolysis Method and Its Application in Exerting Antioxidant Activity of Walnut Protein |
title_fullStr | A Two-Stage Enzymolysis Method and Its Application in Exerting Antioxidant Activity of Walnut Protein |
title_full_unstemmed | A Two-Stage Enzymolysis Method and Its Application in Exerting Antioxidant Activity of Walnut Protein |
title_short | A Two-Stage Enzymolysis Method and Its Application in Exerting Antioxidant Activity of Walnut Protein |
title_sort | two-stage enzymolysis method and its application in exerting antioxidant activity of walnut protein |
topic | Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046995/ https://www.ncbi.nlm.nih.gov/pubmed/35495928 http://dx.doi.org/10.3389/fnut.2022.889434 |
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