Isolation of macrophages from mouse skin wounds for single-cell RNA sequencing

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Understanding macrophage heterogeneity in tissue repair is a major challenge. Here, we describe a protocol that combines isolation of immune cells from skin wounds with subsequent flow-cytometry-based sorting of wound macrophages and single-cell RNA sequencing. We use a modified version of the origi...

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Detalles Bibliográficos
Autores principales: Willenborg, Sebastian, Roscito, Juliana G., Gerbaulet, Alexander, Roers, Axel, Dahl, Andreas, Eming, Sabine A., Reinhardt, Susanne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046999/
https://www.ncbi.nlm.nih.gov/pubmed/35496783
http://dx.doi.org/10.1016/j.xpro.2022.101337
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