Cargando…

Genome-wide identification and expression analysis of magnesium transporter gene family in grape (Vitis vinifera)

BACKGROUND: Magnesium ion is one of the essential mineral elements for plant growth and development, which participates in a variety of physiological and biochemical processes. Since there is no report on the research of magnesium ion transporter in grape, the study of the structure and function of...

Descripción completa

Detalles Bibliográficos
Autores principales: Ge, Mengqing, Zhong, Rong, Sadeghnezhad, Ehsan, Hakeem, Abdul, Xiao, Xin, Wang, Peipei, Fang, Jinggui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047265/
https://www.ncbi.nlm.nih.gov/pubmed/35477360
http://dx.doi.org/10.1186/s12870-022-03599-5
_version_ 1784695687119634432
author Ge, Mengqing
Zhong, Rong
Sadeghnezhad, Ehsan
Hakeem, Abdul
Xiao, Xin
Wang, Peipei
Fang, Jinggui
author_facet Ge, Mengqing
Zhong, Rong
Sadeghnezhad, Ehsan
Hakeem, Abdul
Xiao, Xin
Wang, Peipei
Fang, Jinggui
author_sort Ge, Mengqing
collection PubMed
description BACKGROUND: Magnesium ion is one of the essential mineral elements for plant growth and development, which participates in a variety of physiological and biochemical processes. Since there is no report on the research of magnesium ion transporter in grape, the study of the structure and function of magnesium ion transporters (MGT) is helpful to understand the dynamic balance mechanism of intracellular magnesium ions and their inter- or intra-cellular activities. RESULT: In this study, we identified the members of MGT protein family in grape and performed the phylogenetic and expression analysis. We have identified nine VvMGT genes in grape genome, which are distributed on eight different chromosomes. Phylogenetic analysis showed that MGT family members of grapes were divided into five subfamilies and had obvious homology with Arabidopsis, maize, and pear. Based on transcriptome data from the web databases, we analyzed the expression patterns of VvMGTs at different development stages and in response to abiotic stresses including waterlogging, drought, salinity, and copper. Using qRT-PCR method, we tested the expression of grape VvMGTs under magnesium and aluminum treatments and found significant changes in VvMGTs expression. In addition, four of the MGT proteins in grape were located in the nucleus. CONCLUSION: Overall, in this study we investigated the structural characteristics, evolution pattern, and expression analysis of VvMGTs in depth, which laid the foundation for further revealing the function of VvMGT genes in grape. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03599-5.
format Online
Article
Text
id pubmed-9047265
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-90472652022-04-29 Genome-wide identification and expression analysis of magnesium transporter gene family in grape (Vitis vinifera) Ge, Mengqing Zhong, Rong Sadeghnezhad, Ehsan Hakeem, Abdul Xiao, Xin Wang, Peipei Fang, Jinggui BMC Plant Biol Research BACKGROUND: Magnesium ion is one of the essential mineral elements for plant growth and development, which participates in a variety of physiological and biochemical processes. Since there is no report on the research of magnesium ion transporter in grape, the study of the structure and function of magnesium ion transporters (MGT) is helpful to understand the dynamic balance mechanism of intracellular magnesium ions and their inter- or intra-cellular activities. RESULT: In this study, we identified the members of MGT protein family in grape and performed the phylogenetic and expression analysis. We have identified nine VvMGT genes in grape genome, which are distributed on eight different chromosomes. Phylogenetic analysis showed that MGT family members of grapes were divided into five subfamilies and had obvious homology with Arabidopsis, maize, and pear. Based on transcriptome data from the web databases, we analyzed the expression patterns of VvMGTs at different development stages and in response to abiotic stresses including waterlogging, drought, salinity, and copper. Using qRT-PCR method, we tested the expression of grape VvMGTs under magnesium and aluminum treatments and found significant changes in VvMGTs expression. In addition, four of the MGT proteins in grape were located in the nucleus. CONCLUSION: Overall, in this study we investigated the structural characteristics, evolution pattern, and expression analysis of VvMGTs in depth, which laid the foundation for further revealing the function of VvMGT genes in grape. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03599-5. BioMed Central 2022-04-28 /pmc/articles/PMC9047265/ /pubmed/35477360 http://dx.doi.org/10.1186/s12870-022-03599-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visithttp://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Ge, Mengqing
Zhong, Rong
Sadeghnezhad, Ehsan
Hakeem, Abdul
Xiao, Xin
Wang, Peipei
Fang, Jinggui
Genome-wide identification and expression analysis of magnesium transporter gene family in grape (Vitis vinifera)
title Genome-wide identification and expression analysis of magnesium transporter gene family in grape (Vitis vinifera)
title_full Genome-wide identification and expression analysis of magnesium transporter gene family in grape (Vitis vinifera)
title_fullStr Genome-wide identification and expression analysis of magnesium transporter gene family in grape (Vitis vinifera)
title_full_unstemmed Genome-wide identification and expression analysis of magnesium transporter gene family in grape (Vitis vinifera)
title_short Genome-wide identification and expression analysis of magnesium transporter gene family in grape (Vitis vinifera)
title_sort genome-wide identification and expression analysis of magnesium transporter gene family in grape (vitis vinifera)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047265/
https://www.ncbi.nlm.nih.gov/pubmed/35477360
http://dx.doi.org/10.1186/s12870-022-03599-5
work_keys_str_mv AT gemengqing genomewideidentificationandexpressionanalysisofmagnesiumtransportergenefamilyingrapevitisvinifera
AT zhongrong genomewideidentificationandexpressionanalysisofmagnesiumtransportergenefamilyingrapevitisvinifera
AT sadeghnezhadehsan genomewideidentificationandexpressionanalysisofmagnesiumtransportergenefamilyingrapevitisvinifera
AT hakeemabdul genomewideidentificationandexpressionanalysisofmagnesiumtransportergenefamilyingrapevitisvinifera
AT xiaoxin genomewideidentificationandexpressionanalysisofmagnesiumtransportergenefamilyingrapevitisvinifera
AT wangpeipei genomewideidentificationandexpressionanalysisofmagnesiumtransportergenefamilyingrapevitisvinifera
AT fangjinggui genomewideidentificationandexpressionanalysisofmagnesiumtransportergenefamilyingrapevitisvinifera