Cargando…
Characterisation of the Mentha canadensis R2R3-MYB transcription factor gene McMIXTA and its involvement in peltate glandular trichome development
BACKGROUND: Mentha canadensis L. has important economic value for the production of essential oils, which are synthesised, secreted and stored in peltate glandular trichomes. As a typical multicellular secretory trichome, glandular trichomes are important biological factories for the synthesis of so...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047286/ https://www.ncbi.nlm.nih.gov/pubmed/35477355 http://dx.doi.org/10.1186/s12870-022-03614-9 |
_version_ | 1784695692125536256 |
---|---|
author | Qi, Xiwu Chen, Zequn Yu, Xu Li, Li Bai, Yang Fang, Hailing Liang, Chengyuan |
author_facet | Qi, Xiwu Chen, Zequn Yu, Xu Li, Li Bai, Yang Fang, Hailing Liang, Chengyuan |
author_sort | Qi, Xiwu |
collection | PubMed |
description | BACKGROUND: Mentha canadensis L. has important economic value for the production of essential oils, which are synthesised, secreted and stored in peltate glandular trichomes. As a typical multicellular secretory trichome, glandular trichomes are important biological factories for the synthesis of some specialised metabolites. However, little is known about the molecular mechanism of glandular trichome development in M. canadensis. RESULTS: In this study, the R2R3-MYB transcription factor gene McMIXTA was isolated to investigate its function in glandular trichome development. Bioinformatics analysis indicated that McMIXTA belonged to the subgroup 9 R2R3-MYB, with a R2R3 DNA-binding domain and conserved subgroup 9 motifs. A subcellular localisation assay indicated that McMIXTA was localised in the nucleus. Transactivation analysis indicated that McMIXTA was a positive regulator, with transactivation regions located between positions N253 and N307. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that McMIXTA formed a complex with McHD-Zip3, a trichome development-related HD-ZIP IV transcription factor. Overexpression of McMIXTA in Mentha × piperita L. caused an increase in peltate glandular trichomes density of approximately 25% on the leaf abaxial surface. CONCLUSIONS: Our results demonstrated that the subgroup 9 R2R3-MYB transcription factor McMIXTA has a positive effect on regulating peltate glandular trichome development and the MIXTA/HD-ZIP IV complexes might be conserved regulators for glandular trichome initiation. These results provide useful information for revealing the regulatory mechanism of multicellular glandular trichome development. |
format | Online Article Text |
id | pubmed-9047286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-90472862022-04-29 Characterisation of the Mentha canadensis R2R3-MYB transcription factor gene McMIXTA and its involvement in peltate glandular trichome development Qi, Xiwu Chen, Zequn Yu, Xu Li, Li Bai, Yang Fang, Hailing Liang, Chengyuan BMC Plant Biol Research BACKGROUND: Mentha canadensis L. has important economic value for the production of essential oils, which are synthesised, secreted and stored in peltate glandular trichomes. As a typical multicellular secretory trichome, glandular trichomes are important biological factories for the synthesis of some specialised metabolites. However, little is known about the molecular mechanism of glandular trichome development in M. canadensis. RESULTS: In this study, the R2R3-MYB transcription factor gene McMIXTA was isolated to investigate its function in glandular trichome development. Bioinformatics analysis indicated that McMIXTA belonged to the subgroup 9 R2R3-MYB, with a R2R3 DNA-binding domain and conserved subgroup 9 motifs. A subcellular localisation assay indicated that McMIXTA was localised in the nucleus. Transactivation analysis indicated that McMIXTA was a positive regulator, with transactivation regions located between positions N253 and N307. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that McMIXTA formed a complex with McHD-Zip3, a trichome development-related HD-ZIP IV transcription factor. Overexpression of McMIXTA in Mentha × piperita L. caused an increase in peltate glandular trichomes density of approximately 25% on the leaf abaxial surface. CONCLUSIONS: Our results demonstrated that the subgroup 9 R2R3-MYB transcription factor McMIXTA has a positive effect on regulating peltate glandular trichome development and the MIXTA/HD-ZIP IV complexes might be conserved regulators for glandular trichome initiation. These results provide useful information for revealing the regulatory mechanism of multicellular glandular trichome development. BioMed Central 2022-04-28 /pmc/articles/PMC9047286/ /pubmed/35477355 http://dx.doi.org/10.1186/s12870-022-03614-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Qi, Xiwu Chen, Zequn Yu, Xu Li, Li Bai, Yang Fang, Hailing Liang, Chengyuan Characterisation of the Mentha canadensis R2R3-MYB transcription factor gene McMIXTA and its involvement in peltate glandular trichome development |
title | Characterisation of the Mentha canadensis R2R3-MYB transcription factor gene McMIXTA and its involvement in peltate glandular trichome development |
title_full | Characterisation of the Mentha canadensis R2R3-MYB transcription factor gene McMIXTA and its involvement in peltate glandular trichome development |
title_fullStr | Characterisation of the Mentha canadensis R2R3-MYB transcription factor gene McMIXTA and its involvement in peltate glandular trichome development |
title_full_unstemmed | Characterisation of the Mentha canadensis R2R3-MYB transcription factor gene McMIXTA and its involvement in peltate glandular trichome development |
title_short | Characterisation of the Mentha canadensis R2R3-MYB transcription factor gene McMIXTA and its involvement in peltate glandular trichome development |
title_sort | characterisation of the mentha canadensis r2r3-myb transcription factor gene mcmixta and its involvement in peltate glandular trichome development |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047286/ https://www.ncbi.nlm.nih.gov/pubmed/35477355 http://dx.doi.org/10.1186/s12870-022-03614-9 |
work_keys_str_mv | AT qixiwu characterisationofthementhacanadensisr2r3mybtranscriptionfactorgenemcmixtaanditsinvolvementinpeltateglandulartrichomedevelopment AT chenzequn characterisationofthementhacanadensisr2r3mybtranscriptionfactorgenemcmixtaanditsinvolvementinpeltateglandulartrichomedevelopment AT yuxu characterisationofthementhacanadensisr2r3mybtranscriptionfactorgenemcmixtaanditsinvolvementinpeltateglandulartrichomedevelopment AT lili characterisationofthementhacanadensisr2r3mybtranscriptionfactorgenemcmixtaanditsinvolvementinpeltateglandulartrichomedevelopment AT baiyang characterisationofthementhacanadensisr2r3mybtranscriptionfactorgenemcmixtaanditsinvolvementinpeltateglandulartrichomedevelopment AT fanghailing characterisationofthementhacanadensisr2r3mybtranscriptionfactorgenemcmixtaanditsinvolvementinpeltateglandulartrichomedevelopment AT liangchengyuan characterisationofthementhacanadensisr2r3mybtranscriptionfactorgenemcmixtaanditsinvolvementinpeltateglandulartrichomedevelopment |