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Mapping the Influence of the Gut Microbiota on Small Molecules across the Microbiome Gut Brain Axis
[Image: see text] Microbes exert influence across the microbiome–gut–brain axis through neurotransmitter production, induction of host immunomodulators, or the release or induction of other microbial or host molecules. Here, we used mass spectrometry imaging (MSI), a label-free imaging tool, to map...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047441/ https://www.ncbi.nlm.nih.gov/pubmed/35262356 http://dx.doi.org/10.1021/jasms.1c00298 |
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author | Hulme, Heather Meikle, Lynsey M. Strittmatter, Nicole Swales, John Hamm, Gregory Brown, Sheila L. Milling, Simon MacDonald, Andrew S. Goodwin, Richard J.A. Burchmore, Richard Wall, Daniel M. |
author_facet | Hulme, Heather Meikle, Lynsey M. Strittmatter, Nicole Swales, John Hamm, Gregory Brown, Sheila L. Milling, Simon MacDonald, Andrew S. Goodwin, Richard J.A. Burchmore, Richard Wall, Daniel M. |
author_sort | Hulme, Heather |
collection | PubMed |
description | [Image: see text] Microbes exert influence across the microbiome–gut–brain axis through neurotransmitter production, induction of host immunomodulators, or the release or induction of other microbial or host molecules. Here, we used mass spectrometry imaging (MSI), a label-free imaging tool, to map molecular changes in the gut and brain in germ-free, antibiotic-treated and control mice. We determined spatial distribution and relative quantification of neurotransmitters and their precursors in response to the microbiome. Using untargeted MSI, we detected a significant change in the levels of four identified small molecules in the brains of germ-free animals compared to controls. However, antibiotic treatment induced no significant changes in these same metabolites in the brain after 1 week of treatment. This work exemplifies the utility of MSI as a tool for the study of known and discovery of novel, mediators of microbiome-gut-brain axis communication. |
format | Online Article Text |
id | pubmed-9047441 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-90474412022-04-29 Mapping the Influence of the Gut Microbiota on Small Molecules across the Microbiome Gut Brain Axis Hulme, Heather Meikle, Lynsey M. Strittmatter, Nicole Swales, John Hamm, Gregory Brown, Sheila L. Milling, Simon MacDonald, Andrew S. Goodwin, Richard J.A. Burchmore, Richard Wall, Daniel M. J Am Soc Mass Spectrom [Image: see text] Microbes exert influence across the microbiome–gut–brain axis through neurotransmitter production, induction of host immunomodulators, or the release or induction of other microbial or host molecules. Here, we used mass spectrometry imaging (MSI), a label-free imaging tool, to map molecular changes in the gut and brain in germ-free, antibiotic-treated and control mice. We determined spatial distribution and relative quantification of neurotransmitters and their precursors in response to the microbiome. Using untargeted MSI, we detected a significant change in the levels of four identified small molecules in the brains of germ-free animals compared to controls. However, antibiotic treatment induced no significant changes in these same metabolites in the brain after 1 week of treatment. This work exemplifies the utility of MSI as a tool for the study of known and discovery of novel, mediators of microbiome-gut-brain axis communication. American Chemical Society 2022-03-09 2022-04-06 /pmc/articles/PMC9047441/ /pubmed/35262356 http://dx.doi.org/10.1021/jasms.1c00298 Text en © 2022 American Society for Mass Spectrometry. Published by American Chemical Society. All rights reserved. https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Hulme, Heather Meikle, Lynsey M. Strittmatter, Nicole Swales, John Hamm, Gregory Brown, Sheila L. Milling, Simon MacDonald, Andrew S. Goodwin, Richard J.A. Burchmore, Richard Wall, Daniel M. Mapping the Influence of the Gut Microbiota on Small Molecules across the Microbiome Gut Brain Axis |
title | Mapping the Influence of the Gut Microbiota on Small
Molecules across the Microbiome Gut Brain Axis |
title_full | Mapping the Influence of the Gut Microbiota on Small
Molecules across the Microbiome Gut Brain Axis |
title_fullStr | Mapping the Influence of the Gut Microbiota on Small
Molecules across the Microbiome Gut Brain Axis |
title_full_unstemmed | Mapping the Influence of the Gut Microbiota on Small
Molecules across the Microbiome Gut Brain Axis |
title_short | Mapping the Influence of the Gut Microbiota on Small
Molecules across the Microbiome Gut Brain Axis |
title_sort | mapping the influence of the gut microbiota on small
molecules across the microbiome gut brain axis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047441/ https://www.ncbi.nlm.nih.gov/pubmed/35262356 http://dx.doi.org/10.1021/jasms.1c00298 |
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