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Blood Flow Disturbance and Morphological Alterations Following the Right Atrial Ligation in the Chick Embryo

Collectively known as congenital heart defects (CHDs), cardiac abnormalities at birth are the most common forms of neonatal defects. Being principally responsible for the heart‘s pumping power, ventricles are particularly affected by developmental abnormalities, such as flow disturbances or genomic...

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Autores principales: Alser, Maha, Salman, Huseyin Enes, Naïja, Azza, Seers, Thomas Daniel, Khan, Talha, Yalcin, Huseyin Cagatay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047544/
https://www.ncbi.nlm.nih.gov/pubmed/35492580
http://dx.doi.org/10.3389/fphys.2022.849603
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author Alser, Maha
Salman, Huseyin Enes
Naïja, Azza
Seers, Thomas Daniel
Khan, Talha
Yalcin, Huseyin Cagatay
author_facet Alser, Maha
Salman, Huseyin Enes
Naïja, Azza
Seers, Thomas Daniel
Khan, Talha
Yalcin, Huseyin Cagatay
author_sort Alser, Maha
collection PubMed
description Collectively known as congenital heart defects (CHDs), cardiac abnormalities at birth are the most common forms of neonatal defects. Being principally responsible for the heart‘s pumping power, ventricles are particularly affected by developmental abnormalities, such as flow disturbances or genomic defects. Hypoplastic Right Heart Syndrome (HRHS) is a rare disease where the right ventricle is underdeveloped. In this study, we introduce a surgical procedure performed on chick embryo, termed right atrial ligation (RAL) for disturbing hemodynamics within the right heart aiming in order to generate an animal model of HRHS. RAL is a new surgical manipulation, similar to the well-studied left atrial ligation (LAL) surgery but it induces the hemodynamic change into the right side of the heart. After inducing RAL, We utilized techniques such as Doppler ultrasound, x-ray micro-CT, histology, and computational fluid dynamics (CFD) analysis, for a comprehensive functional and structural analysis of a developing heart. Our results displayed that RAL does not induce severe flow disturbance and ventricular abnormalities consistent with clinical findings. This study allows us to better understand the hemodynamics-driven CHD development and sensitivities of ventricles under disturbed flows.
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spelling pubmed-90475442022-04-29 Blood Flow Disturbance and Morphological Alterations Following the Right Atrial Ligation in the Chick Embryo Alser, Maha Salman, Huseyin Enes Naïja, Azza Seers, Thomas Daniel Khan, Talha Yalcin, Huseyin Cagatay Front Physiol Physiology Collectively known as congenital heart defects (CHDs), cardiac abnormalities at birth are the most common forms of neonatal defects. Being principally responsible for the heart‘s pumping power, ventricles are particularly affected by developmental abnormalities, such as flow disturbances or genomic defects. Hypoplastic Right Heart Syndrome (HRHS) is a rare disease where the right ventricle is underdeveloped. In this study, we introduce a surgical procedure performed on chick embryo, termed right atrial ligation (RAL) for disturbing hemodynamics within the right heart aiming in order to generate an animal model of HRHS. RAL is a new surgical manipulation, similar to the well-studied left atrial ligation (LAL) surgery but it induces the hemodynamic change into the right side of the heart. After inducing RAL, We utilized techniques such as Doppler ultrasound, x-ray micro-CT, histology, and computational fluid dynamics (CFD) analysis, for a comprehensive functional and structural analysis of a developing heart. Our results displayed that RAL does not induce severe flow disturbance and ventricular abnormalities consistent with clinical findings. This study allows us to better understand the hemodynamics-driven CHD development and sensitivities of ventricles under disturbed flows. Frontiers Media S.A. 2022-04-14 /pmc/articles/PMC9047544/ /pubmed/35492580 http://dx.doi.org/10.3389/fphys.2022.849603 Text en Copyright © 2022 Alser, Salman, Naïja, Seers, Khan and Yalcin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Alser, Maha
Salman, Huseyin Enes
Naïja, Azza
Seers, Thomas Daniel
Khan, Talha
Yalcin, Huseyin Cagatay
Blood Flow Disturbance and Morphological Alterations Following the Right Atrial Ligation in the Chick Embryo
title Blood Flow Disturbance and Morphological Alterations Following the Right Atrial Ligation in the Chick Embryo
title_full Blood Flow Disturbance and Morphological Alterations Following the Right Atrial Ligation in the Chick Embryo
title_fullStr Blood Flow Disturbance and Morphological Alterations Following the Right Atrial Ligation in the Chick Embryo
title_full_unstemmed Blood Flow Disturbance and Morphological Alterations Following the Right Atrial Ligation in the Chick Embryo
title_short Blood Flow Disturbance and Morphological Alterations Following the Right Atrial Ligation in the Chick Embryo
title_sort blood flow disturbance and morphological alterations following the right atrial ligation in the chick embryo
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047544/
https://www.ncbi.nlm.nih.gov/pubmed/35492580
http://dx.doi.org/10.3389/fphys.2022.849603
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