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Isolation and Phylogenetic Analysis of a Hunnivirus Strain in Water Buffaloes From China
In recent years, hunniviruses have been reported in a variety of animal species from many countries. Here, hunnivirus was detected in fecal samples from water buffaloes and named as BufHuV-GX-2106. The samples were inoculated into cultures of MDBK cells supplemented with TPCK trypsin and the BufHuV-...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047669/ https://www.ncbi.nlm.nih.gov/pubmed/35498751 http://dx.doi.org/10.3389/fvets.2022.851743 |
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author | Liu, Haifeng Zhu, Xinyue Dong, Qinting Qiao, Chengpeng Luo, Yuhang Liu, Yi Zou, Yanlin Liu, Huanghao Wu, Cuilan Su, Jieyu Peng, Hao Ouyang, Kang Chen, Ying Li, Jun Wei, Zuzhang Huang, Weijian |
author_facet | Liu, Haifeng Zhu, Xinyue Dong, Qinting Qiao, Chengpeng Luo, Yuhang Liu, Yi Zou, Yanlin Liu, Huanghao Wu, Cuilan Su, Jieyu Peng, Hao Ouyang, Kang Chen, Ying Li, Jun Wei, Zuzhang Huang, Weijian |
author_sort | Liu, Haifeng |
collection | PubMed |
description | In recent years, hunniviruses have been reported in a variety of animal species from many countries. Here, hunnivirus was detected in fecal samples from water buffaloes and named as BufHuV-GX-2106. The samples were inoculated into cultures of MDBK cells supplemented with TPCK trypsin and the BufHuV-GX-2106 strain was stably passaged and replicated. Electron microscopic analysis showed the BufHuV-GX-2106 virus particles were spherical and 20~30 nm in diameter. The complete genome of a plaque purified sample of BufHuV-GX-2106 was determined and analyzed. Genomic analysis revealed that the whole sequence of BufHuV-GX-2106 was ~7,601 nucleotides (nt) in length and consisted of a large open reading frame of 6,759nt, a 5′UTR, a 3'UTR and a poly(A) tail. The complete genome sequence of BufHuV-GX-2106 shares 68-85% nucleotide identities with other known hunnivirus strains, indicating high genetic heterogeneity among these viruses. Phylogenetic analysis showed that BufHuV-GX-2106 belonged to the Hunnivirus A species and was more closely related to ovine hunnivirus than other known viruses of this type. This study describes the first isolation and complete genome sequence of a hunnivirus strain from water buffaloes. In addition, this study will help to understand the mechanisms involved in the pathogenesis of Hunnivirus A among different animal species. |
format | Online Article Text |
id | pubmed-9047669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90476692022-04-29 Isolation and Phylogenetic Analysis of a Hunnivirus Strain in Water Buffaloes From China Liu, Haifeng Zhu, Xinyue Dong, Qinting Qiao, Chengpeng Luo, Yuhang Liu, Yi Zou, Yanlin Liu, Huanghao Wu, Cuilan Su, Jieyu Peng, Hao Ouyang, Kang Chen, Ying Li, Jun Wei, Zuzhang Huang, Weijian Front Vet Sci Veterinary Science In recent years, hunniviruses have been reported in a variety of animal species from many countries. Here, hunnivirus was detected in fecal samples from water buffaloes and named as BufHuV-GX-2106. The samples were inoculated into cultures of MDBK cells supplemented with TPCK trypsin and the BufHuV-GX-2106 strain was stably passaged and replicated. Electron microscopic analysis showed the BufHuV-GX-2106 virus particles were spherical and 20~30 nm in diameter. The complete genome of a plaque purified sample of BufHuV-GX-2106 was determined and analyzed. Genomic analysis revealed that the whole sequence of BufHuV-GX-2106 was ~7,601 nucleotides (nt) in length and consisted of a large open reading frame of 6,759nt, a 5′UTR, a 3'UTR and a poly(A) tail. The complete genome sequence of BufHuV-GX-2106 shares 68-85% nucleotide identities with other known hunnivirus strains, indicating high genetic heterogeneity among these viruses. Phylogenetic analysis showed that BufHuV-GX-2106 belonged to the Hunnivirus A species and was more closely related to ovine hunnivirus than other known viruses of this type. This study describes the first isolation and complete genome sequence of a hunnivirus strain from water buffaloes. In addition, this study will help to understand the mechanisms involved in the pathogenesis of Hunnivirus A among different animal species. Frontiers Media S.A. 2022-04-14 /pmc/articles/PMC9047669/ /pubmed/35498751 http://dx.doi.org/10.3389/fvets.2022.851743 Text en Copyright © 2022 Liu, Zhu, Dong, Qiao, Luo, Liu, Zou, Liu, Wu, Su, Peng, Ouyang, Chen, Li, Wei and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Liu, Haifeng Zhu, Xinyue Dong, Qinting Qiao, Chengpeng Luo, Yuhang Liu, Yi Zou, Yanlin Liu, Huanghao Wu, Cuilan Su, Jieyu Peng, Hao Ouyang, Kang Chen, Ying Li, Jun Wei, Zuzhang Huang, Weijian Isolation and Phylogenetic Analysis of a Hunnivirus Strain in Water Buffaloes From China |
title | Isolation and Phylogenetic Analysis of a Hunnivirus Strain in Water Buffaloes From China |
title_full | Isolation and Phylogenetic Analysis of a Hunnivirus Strain in Water Buffaloes From China |
title_fullStr | Isolation and Phylogenetic Analysis of a Hunnivirus Strain in Water Buffaloes From China |
title_full_unstemmed | Isolation and Phylogenetic Analysis of a Hunnivirus Strain in Water Buffaloes From China |
title_short | Isolation and Phylogenetic Analysis of a Hunnivirus Strain in Water Buffaloes From China |
title_sort | isolation and phylogenetic analysis of a hunnivirus strain in water buffaloes from china |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047669/ https://www.ncbi.nlm.nih.gov/pubmed/35498751 http://dx.doi.org/10.3389/fvets.2022.851743 |
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