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Serglycin Is Involved in TGF-β Induced Epithelial-Mesenchymal Transition and Is Highly Expressed by Immune Cells in Breast Cancer Tissue

Serglycin is a proteoglycan highly expressed by immune cells, in which its functions are linked to storage, secretion, transport, and protection of chemokines, proteases, histamine, growth factors, and other bioactive molecules. In recent years, it has been demonstrated that serglycin is also expres...

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Autores principales: Tellez-Gabriel, Marta, Tekpli, Xavier, Reine, Trine M., Hegge, Beate, Nielsen, Stephanie R., Chen, Meng, Moi, Line, Normann, Lisa Svartdal, Busund, Lill-Tove R., Calin, George A., Mælandsmo, Gunhild M., Perander, Maria, Theocharis, Achilleas D., Kolset, Svein O., Knutsen, Erik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047906/
https://www.ncbi.nlm.nih.gov/pubmed/35494005
http://dx.doi.org/10.3389/fonc.2022.868868
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author Tellez-Gabriel, Marta
Tekpli, Xavier
Reine, Trine M.
Hegge, Beate
Nielsen, Stephanie R.
Chen, Meng
Moi, Line
Normann, Lisa Svartdal
Busund, Lill-Tove R.
Calin, George A.
Mælandsmo, Gunhild M.
Perander, Maria
Theocharis, Achilleas D.
Kolset, Svein O.
Knutsen, Erik
author_facet Tellez-Gabriel, Marta
Tekpli, Xavier
Reine, Trine M.
Hegge, Beate
Nielsen, Stephanie R.
Chen, Meng
Moi, Line
Normann, Lisa Svartdal
Busund, Lill-Tove R.
Calin, George A.
Mælandsmo, Gunhild M.
Perander, Maria
Theocharis, Achilleas D.
Kolset, Svein O.
Knutsen, Erik
author_sort Tellez-Gabriel, Marta
collection PubMed
description Serglycin is a proteoglycan highly expressed by immune cells, in which its functions are linked to storage, secretion, transport, and protection of chemokines, proteases, histamine, growth factors, and other bioactive molecules. In recent years, it has been demonstrated that serglycin is also expressed by several other cell types, such as endothelial cells, muscle cells, and multiple types of cancer cells. Here, we show that serglycin expression is upregulated in transforming growth factor beta (TGF-β) induced epithelial-mesenchymal transition (EMT). Functional studies provide evidence that serglycin plays an important role in the regulation of the transition between the epithelial and mesenchymal phenotypes, and it is a significant EMT marker gene. We further find that serglycin is more expressed by breast cancer cell lines with a mesenchymal phenotype as well as the basal-like subtype of breast cancers. By examining immune staining and single cell sequencing data of breast cancer tissue, we show that serglycin is highly expressed by infiltrating immune cells in breast tumor tissue.
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spelling pubmed-90479062022-04-29 Serglycin Is Involved in TGF-β Induced Epithelial-Mesenchymal Transition and Is Highly Expressed by Immune Cells in Breast Cancer Tissue Tellez-Gabriel, Marta Tekpli, Xavier Reine, Trine M. Hegge, Beate Nielsen, Stephanie R. Chen, Meng Moi, Line Normann, Lisa Svartdal Busund, Lill-Tove R. Calin, George A. Mælandsmo, Gunhild M. Perander, Maria Theocharis, Achilleas D. Kolset, Svein O. Knutsen, Erik Front Oncol Oncology Serglycin is a proteoglycan highly expressed by immune cells, in which its functions are linked to storage, secretion, transport, and protection of chemokines, proteases, histamine, growth factors, and other bioactive molecules. In recent years, it has been demonstrated that serglycin is also expressed by several other cell types, such as endothelial cells, muscle cells, and multiple types of cancer cells. Here, we show that serglycin expression is upregulated in transforming growth factor beta (TGF-β) induced epithelial-mesenchymal transition (EMT). Functional studies provide evidence that serglycin plays an important role in the regulation of the transition between the epithelial and mesenchymal phenotypes, and it is a significant EMT marker gene. We further find that serglycin is more expressed by breast cancer cell lines with a mesenchymal phenotype as well as the basal-like subtype of breast cancers. By examining immune staining and single cell sequencing data of breast cancer tissue, we show that serglycin is highly expressed by infiltrating immune cells in breast tumor tissue. Frontiers Media S.A. 2022-04-14 /pmc/articles/PMC9047906/ /pubmed/35494005 http://dx.doi.org/10.3389/fonc.2022.868868 Text en Copyright © 2022 Tellez-Gabriel, Tekpli, Reine, Hegge, Nielsen, Chen, Moi, Normann, Busund, Calin, Mælandsmo, Perander, Theocharis, Kolset and Knutsen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Tellez-Gabriel, Marta
Tekpli, Xavier
Reine, Trine M.
Hegge, Beate
Nielsen, Stephanie R.
Chen, Meng
Moi, Line
Normann, Lisa Svartdal
Busund, Lill-Tove R.
Calin, George A.
Mælandsmo, Gunhild M.
Perander, Maria
Theocharis, Achilleas D.
Kolset, Svein O.
Knutsen, Erik
Serglycin Is Involved in TGF-β Induced Epithelial-Mesenchymal Transition and Is Highly Expressed by Immune Cells in Breast Cancer Tissue
title Serglycin Is Involved in TGF-β Induced Epithelial-Mesenchymal Transition and Is Highly Expressed by Immune Cells in Breast Cancer Tissue
title_full Serglycin Is Involved in TGF-β Induced Epithelial-Mesenchymal Transition and Is Highly Expressed by Immune Cells in Breast Cancer Tissue
title_fullStr Serglycin Is Involved in TGF-β Induced Epithelial-Mesenchymal Transition and Is Highly Expressed by Immune Cells in Breast Cancer Tissue
title_full_unstemmed Serglycin Is Involved in TGF-β Induced Epithelial-Mesenchymal Transition and Is Highly Expressed by Immune Cells in Breast Cancer Tissue
title_short Serglycin Is Involved in TGF-β Induced Epithelial-Mesenchymal Transition and Is Highly Expressed by Immune Cells in Breast Cancer Tissue
title_sort serglycin is involved in tgf-β induced epithelial-mesenchymal transition and is highly expressed by immune cells in breast cancer tissue
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047906/
https://www.ncbi.nlm.nih.gov/pubmed/35494005
http://dx.doi.org/10.3389/fonc.2022.868868
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