The speciation of inorganic arsenic in soil and vegetables irrigated with treated municipal wastewater

In this research, an environmental friendly, green and efficient sample preparation method using vortex-assisted microextraction based on a deep eutectic solvent (VAME-DES) followed by graphite furnace atomic absorption spectrometry (GFAAS) was developed for the preconcentration and determination of As(iii)/As(v) and total inorganic arsenic in soil and vegetables irrigated with treated municipal wastewater from Tehran and Kermanshah, Iran. In the proposed method, a novel DES, characterized by its low density, was prepared by mixing choline chloride and citric acid monohydrate at a molar ratio of 1 : 1. Under optimal conditions, the proposed method enabled the achievement of a good enrichment factor of 175. The calibration graph was linear in the range of 0.3–100 μg kg(−1) and the limit of detection (LOD) was 0.10 μg kg(−1). The repeatability and reproducibility of the method based on seven replicate measurements of 50 μg kg(−1) As(iii) in analysed samples were 4.2% and 6.5, respectively. The relative recoveries from soil and vegetables that were spiked with different levels of As(iii) and As(v) were 94.2–104.3 and 91.0–107.0%, respectively. The main advantage of the proposed method is the use of a non-toxic and non-volatile DES instead of volatile organic solvents. The accuracy of the proposed procedure was also assessed by the speciation of arsenic in two standard reference materials (GBW10014 cabbage and GBW10015 spinach). The extraction methodology is simple, rapid, cheap and green, since only small amounts of non-toxic solvents are necessary.