Optimized protocol for stable isotope tracing and steady-state metabolomics in mouse HER2+ breast cancer brain metastasis

Analyzing the metabolic dependencies of tumor cells is vital for cancer diagnosis and treatment. Here, we describe a protocol for (13)C-stable glucose and glutamine isotope tracing in mice HER2+ breast cancer brain metastatic lesions. We describe how to inject cancer cells intracardially to generate...

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Detalles Bibliográficos
Autores principales: Parida, Pravat Kumar, Marquez-Palencia, Mauricio, Kaushik, Akash K., Kim, Kangsan, Nair, Vidhya, Sudderth, Jessica, Vu, Hieu, Zacharias, Lauren, DeBerardinis, Ralph, Malladi, Srinivas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048131/
https://www.ncbi.nlm.nih.gov/pubmed/35496802
http://dx.doi.org/10.1016/j.xpro.2022.101345

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