Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning

A resonant-scanning multiphoton optical microscope (MPM) with a millimeter-scale field-of-view (FOV) often encounters a poor Nyquist figure-of-merit (NFOM), leading to an aliasing effect owing to limited effective voxel-sampling rate. In this protocol, we provide a design guideline to enable high-NF...

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Detalles Bibliográficos
Autores principales: Borah, Bhaskar Jyoti, Sun, Chi-Kuang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048148/
https://www.ncbi.nlm.nih.gov/pubmed/35496804
http://dx.doi.org/10.1016/j.xpro.2022.101330
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author Borah, Bhaskar Jyoti
Sun, Chi-Kuang
author_facet Borah, Bhaskar Jyoti
Sun, Chi-Kuang
author_sort Borah, Bhaskar Jyoti
collection PubMed
description A resonant-scanning multiphoton optical microscope (MPM) with a millimeter-scale field-of-view (FOV) often encounters a poor Nyquist figure-of-merit (NFOM), leading to an aliasing effect owing to limited effective voxel-sampling rate. In this protocol, we provide a design guideline to enable high-NFOM MPM imaging while simultaneously securing a large FOV/digital-resolution ratio and a fast resonant raster-scanning speed. We further provide a free version of our custom acquisition software to assist with a smooth and easy construction process. For complete details on the use and execution of this protocol, please refer to Borah et al. (2021).
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spelling pubmed-90481482022-04-29 Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning Borah, Bhaskar Jyoti Sun, Chi-Kuang STAR Protoc Protocol A resonant-scanning multiphoton optical microscope (MPM) with a millimeter-scale field-of-view (FOV) often encounters a poor Nyquist figure-of-merit (NFOM), leading to an aliasing effect owing to limited effective voxel-sampling rate. In this protocol, we provide a design guideline to enable high-NFOM MPM imaging while simultaneously securing a large FOV/digital-resolution ratio and a fast resonant raster-scanning speed. We further provide a free version of our custom acquisition software to assist with a smooth and easy construction process. For complete details on the use and execution of this protocol, please refer to Borah et al. (2021). Elsevier 2022-04-22 /pmc/articles/PMC9048148/ /pubmed/35496804 http://dx.doi.org/10.1016/j.xpro.2022.101330 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Borah, Bhaskar Jyoti
Sun, Chi-Kuang
Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning
title Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning
title_full Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning
title_fullStr Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning
title_full_unstemmed Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning
title_short Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning
title_sort construction of a high-nfom multiphoton microscope with large-angle resonant raster scanning
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048148/
https://www.ncbi.nlm.nih.gov/pubmed/35496804
http://dx.doi.org/10.1016/j.xpro.2022.101330
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