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High-Force Application by a Nanoscale DNA Force Spectrometer

[Image: see text] The ability to apply and measure high forces (>10 pN) on the nanometer scale is critical to the development of nanomedicine, molecular robotics, and the understanding of biological processes such as chromatin condensation, membrane deformation, and viral packaging. Established f...

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Autores principales: Darcy, Michael, Crocker, Kyle, Wang, Yuchen, Le, Jenny V., Mohammadiroozbahani, Golbarg, Abdelhamid, Mahmoud A. S., Craggs, Timothy D., Castro, Carlos E., Bundschuh, Ralf, Poirier, Michael G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048690/
https://www.ncbi.nlm.nih.gov/pubmed/35385658
http://dx.doi.org/10.1021/acsnano.1c10698
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author Darcy, Michael
Crocker, Kyle
Wang, Yuchen
Le, Jenny V.
Mohammadiroozbahani, Golbarg
Abdelhamid, Mahmoud A. S.
Craggs, Timothy D.
Castro, Carlos E.
Bundschuh, Ralf
Poirier, Michael G.
author_facet Darcy, Michael
Crocker, Kyle
Wang, Yuchen
Le, Jenny V.
Mohammadiroozbahani, Golbarg
Abdelhamid, Mahmoud A. S.
Craggs, Timothy D.
Castro, Carlos E.
Bundschuh, Ralf
Poirier, Michael G.
author_sort Darcy, Michael
collection PubMed
description [Image: see text] The ability to apply and measure high forces (>10 pN) on the nanometer scale is critical to the development of nanomedicine, molecular robotics, and the understanding of biological processes such as chromatin condensation, membrane deformation, and viral packaging. Established force spectroscopy techniques including optical traps, magnetic tweezers, and atomic force microscopy rely on micron-sized or larger handles to apply forces, limiting their applications within constrained geometries including cellular environments and nanofluidic devices. A promising alternative to these approaches is DNA-based molecular calipers. However, this approach is currently limited to forces on the scale of a few piconewtons. To study the force application capabilities of DNA devices, we implemented DNA origami nanocalipers with tunable mechanical properties in a geometry that allows application of force to rupture a DNA duplex. We integrated static and dynamic single-molecule characterization methods and statistical mechanical modeling to quantify the device properties including force output and dynamic range. We found that the thermally driven dynamics of the device are capable of applying forces of at least 20 piconewtons with a nanometer-scale dynamic range. These characteristics could eventually be used to study other biomolecular processes such as protein unfolding or to control high-affinity interactions in nanomechanical devices or molecular robots.
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spelling pubmed-90486902023-04-06 High-Force Application by a Nanoscale DNA Force Spectrometer Darcy, Michael Crocker, Kyle Wang, Yuchen Le, Jenny V. Mohammadiroozbahani, Golbarg Abdelhamid, Mahmoud A. S. Craggs, Timothy D. Castro, Carlos E. Bundschuh, Ralf Poirier, Michael G. ACS Nano [Image: see text] The ability to apply and measure high forces (>10 pN) on the nanometer scale is critical to the development of nanomedicine, molecular robotics, and the understanding of biological processes such as chromatin condensation, membrane deformation, and viral packaging. Established force spectroscopy techniques including optical traps, magnetic tweezers, and atomic force microscopy rely on micron-sized or larger handles to apply forces, limiting their applications within constrained geometries including cellular environments and nanofluidic devices. A promising alternative to these approaches is DNA-based molecular calipers. However, this approach is currently limited to forces on the scale of a few piconewtons. To study the force application capabilities of DNA devices, we implemented DNA origami nanocalipers with tunable mechanical properties in a geometry that allows application of force to rupture a DNA duplex. We integrated static and dynamic single-molecule characterization methods and statistical mechanical modeling to quantify the device properties including force output and dynamic range. We found that the thermally driven dynamics of the device are capable of applying forces of at least 20 piconewtons with a nanometer-scale dynamic range. These characteristics could eventually be used to study other biomolecular processes such as protein unfolding or to control high-affinity interactions in nanomechanical devices or molecular robots. American Chemical Society 2022-04-06 2022-04-26 /pmc/articles/PMC9048690/ /pubmed/35385658 http://dx.doi.org/10.1021/acsnano.1c10698 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Darcy, Michael
Crocker, Kyle
Wang, Yuchen
Le, Jenny V.
Mohammadiroozbahani, Golbarg
Abdelhamid, Mahmoud A. S.
Craggs, Timothy D.
Castro, Carlos E.
Bundschuh, Ralf
Poirier, Michael G.
High-Force Application by a Nanoscale DNA Force Spectrometer
title High-Force Application by a Nanoscale DNA Force Spectrometer
title_full High-Force Application by a Nanoscale DNA Force Spectrometer
title_fullStr High-Force Application by a Nanoscale DNA Force Spectrometer
title_full_unstemmed High-Force Application by a Nanoscale DNA Force Spectrometer
title_short High-Force Application by a Nanoscale DNA Force Spectrometer
title_sort high-force application by a nanoscale dna force spectrometer
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048690/
https://www.ncbi.nlm.nih.gov/pubmed/35385658
http://dx.doi.org/10.1021/acsnano.1c10698
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