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A new bio-compatible Cd(2+)-selective nanostructured fluorescent imprinted polymer for cadmium ion sensing in aqueous media and its application in bio imaging in Vero cells
Cadmium is a very toxic element found in various aqueous samples. The majority of the highly selective fluorescent ligands, designed for cadmium ion sensing, are hydrophobic compounds, thus making them inactive in aqueous media. Fluorescent imprinted polymers, synthesized by the proficient combinati...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048730/ https://www.ncbi.nlm.nih.gov/pubmed/35492647 http://dx.doi.org/10.1039/c9ra06910k |
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author | Alizadeh, Taher Sharifi, Amir Reza Ganjali, Mohammad Reza |
author_facet | Alizadeh, Taher Sharifi, Amir Reza Ganjali, Mohammad Reza |
author_sort | Alizadeh, Taher |
collection | PubMed |
description | Cadmium is a very toxic element found in various aqueous samples. The majority of the highly selective fluorescent ligands, designed for cadmium ion sensing, are hydrophobic compounds, thus making them inactive in aqueous media. Fluorescent imprinted polymers, synthesized by the proficient combination of hydrophilic functional monomers and hydrophobic ligands, may give a new and highly selective opportunity for utilizing most fluorescent ligands for toxic metal ion sensing in aqueous media. A novel fluorescent Cd(2+)-imprinted polymer was synthesized based on the co-polymerization of a mixture of acryl amide, vinyl benzene and ethylene glycol dimethacrylate in the presence of a 5-((3-hydroxynaphthalen-2-yl)methylene)pyrimidine-2,4,6(1,3,5)-trione (HMPT)-Cd(2+) complex. The polymer was characterized by FT-IR spectroscopy, scanning electron microscopy and thermogravimetric analysis. Cadmium ion recognition by IIP created a new emission peak at about 502 nm based on the ICT mechanism, which was different from the emission peak of IIP in the absence of Cd(2+) (440 nm). The non-imprinted polymer showed a fluorescence emission at about 500 nm, which was not affected by Cd(2+), highlighting the recognition sites of IIP. The opto-sensor (IIP) exhibited a dynamic linear response range of 10–0.05 μM with the limit of detection (LOD) and quantification (LOQ) of 12.3 and 41 nM, respectively. Also, the relative standard deviation (RSD) of 3 separate determinations was 3.68%. Moreover, the developed chemosensor was highly selective for Cd(2+) since the IIP fluorescence was not affected by the presence of other metal ions such as Zn(2+), Cu(+), Mn(2+), Co(2+), Ni(2+), and Pb(2+). The synthesized IIP can be used as a fluorescent probe for cadmium detection in live cells because of its minor cytotoxic effect on them. |
format | Online Article Text |
id | pubmed-9048730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90487302022-04-28 A new bio-compatible Cd(2+)-selective nanostructured fluorescent imprinted polymer for cadmium ion sensing in aqueous media and its application in bio imaging in Vero cells Alizadeh, Taher Sharifi, Amir Reza Ganjali, Mohammad Reza RSC Adv Chemistry Cadmium is a very toxic element found in various aqueous samples. The majority of the highly selective fluorescent ligands, designed for cadmium ion sensing, are hydrophobic compounds, thus making them inactive in aqueous media. Fluorescent imprinted polymers, synthesized by the proficient combination of hydrophilic functional monomers and hydrophobic ligands, may give a new and highly selective opportunity for utilizing most fluorescent ligands for toxic metal ion sensing in aqueous media. A novel fluorescent Cd(2+)-imprinted polymer was synthesized based on the co-polymerization of a mixture of acryl amide, vinyl benzene and ethylene glycol dimethacrylate in the presence of a 5-((3-hydroxynaphthalen-2-yl)methylene)pyrimidine-2,4,6(1,3,5)-trione (HMPT)-Cd(2+) complex. The polymer was characterized by FT-IR spectroscopy, scanning electron microscopy and thermogravimetric analysis. Cadmium ion recognition by IIP created a new emission peak at about 502 nm based on the ICT mechanism, which was different from the emission peak of IIP in the absence of Cd(2+) (440 nm). The non-imprinted polymer showed a fluorescence emission at about 500 nm, which was not affected by Cd(2+), highlighting the recognition sites of IIP. The opto-sensor (IIP) exhibited a dynamic linear response range of 10–0.05 μM with the limit of detection (LOD) and quantification (LOQ) of 12.3 and 41 nM, respectively. Also, the relative standard deviation (RSD) of 3 separate determinations was 3.68%. Moreover, the developed chemosensor was highly selective for Cd(2+) since the IIP fluorescence was not affected by the presence of other metal ions such as Zn(2+), Cu(+), Mn(2+), Co(2+), Ni(2+), and Pb(2+). The synthesized IIP can be used as a fluorescent probe for cadmium detection in live cells because of its minor cytotoxic effect on them. The Royal Society of Chemistry 2020-01-24 /pmc/articles/PMC9048730/ /pubmed/35492647 http://dx.doi.org/10.1039/c9ra06910k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Alizadeh, Taher Sharifi, Amir Reza Ganjali, Mohammad Reza A new bio-compatible Cd(2+)-selective nanostructured fluorescent imprinted polymer for cadmium ion sensing in aqueous media and its application in bio imaging in Vero cells |
title | A new bio-compatible Cd(2+)-selective nanostructured fluorescent imprinted polymer for cadmium ion sensing in aqueous media and its application in bio imaging in Vero cells |
title_full | A new bio-compatible Cd(2+)-selective nanostructured fluorescent imprinted polymer for cadmium ion sensing in aqueous media and its application in bio imaging in Vero cells |
title_fullStr | A new bio-compatible Cd(2+)-selective nanostructured fluorescent imprinted polymer for cadmium ion sensing in aqueous media and its application in bio imaging in Vero cells |
title_full_unstemmed | A new bio-compatible Cd(2+)-selective nanostructured fluorescent imprinted polymer for cadmium ion sensing in aqueous media and its application in bio imaging in Vero cells |
title_short | A new bio-compatible Cd(2+)-selective nanostructured fluorescent imprinted polymer for cadmium ion sensing in aqueous media and its application in bio imaging in Vero cells |
title_sort | new bio-compatible cd(2+)-selective nanostructured fluorescent imprinted polymer for cadmium ion sensing in aqueous media and its application in bio imaging in vero cells |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048730/ https://www.ncbi.nlm.nih.gov/pubmed/35492647 http://dx.doi.org/10.1039/c9ra06910k |
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