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Diversity of Anaerobic Methane Oxidizers in the Cold Seep Sediments of the Okinawa Trough
Active cold seeps in the Okinawa Trough (OT) have been widely identified, but the sediment microbial communities associated with these sites are still poorly understood. Here, we investigated the distribution and biomass of the microbial communities, particularly those associated with the anaerobic...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048799/ https://www.ncbi.nlm.nih.gov/pubmed/35495656 http://dx.doi.org/10.3389/fmicb.2022.819187 |
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author | Chen, Ye Xu, Cuiling Wu, Nengyou Sun, Zhilei Liu, Changling Zhen, Yu Xin, Youzhi Zhang, Xilin Geng, Wei Cao, Hong Zhai, Bin Li, Jing Qin, Shuangshuang Zhou, Yucheng |
author_facet | Chen, Ye Xu, Cuiling Wu, Nengyou Sun, Zhilei Liu, Changling Zhen, Yu Xin, Youzhi Zhang, Xilin Geng, Wei Cao, Hong Zhai, Bin Li, Jing Qin, Shuangshuang Zhou, Yucheng |
author_sort | Chen, Ye |
collection | PubMed |
description | Active cold seeps in the Okinawa Trough (OT) have been widely identified, but the sediment microbial communities associated with these sites are still poorly understood. Here, we investigated the distribution and biomass of the microbial communities, particularly those associated with the anaerobic oxidation of methane (AOM), in sediments from an active cold seep in the mid-Okinawa Trough. Methane-oxidizing archaea, including ANME-1a, ANME-1b, ANME-2a/b, ANME-2c, and ANME-3, were detected in the OT cold seep sediments. Vertical stratification of anaerobic methanotrophic archaea (ANME) communities was observed in the following order: ANME-3, ANME-1a, and ANME-1b. In addition, the abundance of methyl coenzyme M reductase A (mcrA) genes corresponded to high levels of dissolved iron, suggesting that methane-metabolizing archaea might participate in iron reduction coupled to methane oxidation (Fe-AOM) in the OT cold seep. Furthermore, the relative abundance of ANME-1a was strongly related to the concentration of dissolved iron, indicating that ANME-1a is a key microbial player for Fe-AOM in the OT cold seep sediments. Co-occurrence analysis revealed that methane-metabolizing microbial communities were mainly associated with heterotrophic microorganisms, such as JS1, Bathy-1, and Bathy-15. |
format | Online Article Text |
id | pubmed-9048799 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90487992022-04-29 Diversity of Anaerobic Methane Oxidizers in the Cold Seep Sediments of the Okinawa Trough Chen, Ye Xu, Cuiling Wu, Nengyou Sun, Zhilei Liu, Changling Zhen, Yu Xin, Youzhi Zhang, Xilin Geng, Wei Cao, Hong Zhai, Bin Li, Jing Qin, Shuangshuang Zhou, Yucheng Front Microbiol Microbiology Active cold seeps in the Okinawa Trough (OT) have been widely identified, but the sediment microbial communities associated with these sites are still poorly understood. Here, we investigated the distribution and biomass of the microbial communities, particularly those associated with the anaerobic oxidation of methane (AOM), in sediments from an active cold seep in the mid-Okinawa Trough. Methane-oxidizing archaea, including ANME-1a, ANME-1b, ANME-2a/b, ANME-2c, and ANME-3, were detected in the OT cold seep sediments. Vertical stratification of anaerobic methanotrophic archaea (ANME) communities was observed in the following order: ANME-3, ANME-1a, and ANME-1b. In addition, the abundance of methyl coenzyme M reductase A (mcrA) genes corresponded to high levels of dissolved iron, suggesting that methane-metabolizing archaea might participate in iron reduction coupled to methane oxidation (Fe-AOM) in the OT cold seep. Furthermore, the relative abundance of ANME-1a was strongly related to the concentration of dissolved iron, indicating that ANME-1a is a key microbial player for Fe-AOM in the OT cold seep sediments. Co-occurrence analysis revealed that methane-metabolizing microbial communities were mainly associated with heterotrophic microorganisms, such as JS1, Bathy-1, and Bathy-15. Frontiers Media S.A. 2022-04-14 /pmc/articles/PMC9048799/ /pubmed/35495656 http://dx.doi.org/10.3389/fmicb.2022.819187 Text en Copyright © 2022 Chen, Xu, Wu, Sun, Liu, Zhen, Xin, Zhang, Geng, Cao, Zhai, Li, Qin and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Chen, Ye Xu, Cuiling Wu, Nengyou Sun, Zhilei Liu, Changling Zhen, Yu Xin, Youzhi Zhang, Xilin Geng, Wei Cao, Hong Zhai, Bin Li, Jing Qin, Shuangshuang Zhou, Yucheng Diversity of Anaerobic Methane Oxidizers in the Cold Seep Sediments of the Okinawa Trough |
title | Diversity of Anaerobic Methane Oxidizers in the Cold Seep Sediments of the Okinawa Trough |
title_full | Diversity of Anaerobic Methane Oxidizers in the Cold Seep Sediments of the Okinawa Trough |
title_fullStr | Diversity of Anaerobic Methane Oxidizers in the Cold Seep Sediments of the Okinawa Trough |
title_full_unstemmed | Diversity of Anaerobic Methane Oxidizers in the Cold Seep Sediments of the Okinawa Trough |
title_short | Diversity of Anaerobic Methane Oxidizers in the Cold Seep Sediments of the Okinawa Trough |
title_sort | diversity of anaerobic methane oxidizers in the cold seep sediments of the okinawa trough |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048799/ https://www.ncbi.nlm.nih.gov/pubmed/35495656 http://dx.doi.org/10.3389/fmicb.2022.819187 |
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