Cargando…

Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds

Biomaterial scaffolds play a critical role in bone tissue engineering. Moreover, 3D printing technology has enormous advantage in the manufacture of bioengineering scaffolds for patient-specific bone defect treatments. In order to provide an aseptic environment for bone regeneration, ε-poly-l-lysine...

Descripción completa

Detalles Bibliográficos
Autores principales: Tian, Lijiao, Zhang, Zhenting, Tian, Bin, Zhang, Xin, Wang, Na
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049012/
https://www.ncbi.nlm.nih.gov/pubmed/35495239
http://dx.doi.org/10.1039/c9ra10275b
_version_ 1784696049953144832
author Tian, Lijiao
Zhang, Zhenting
Tian, Bin
Zhang, Xin
Wang, Na
author_facet Tian, Lijiao
Zhang, Zhenting
Tian, Bin
Zhang, Xin
Wang, Na
author_sort Tian, Lijiao
collection PubMed
description Biomaterial scaffolds play a critical role in bone tissue engineering. Moreover, 3D printing technology has enormous advantage in the manufacture of bioengineering scaffolds for patient-specific bone defect treatments. In order to provide an aseptic environment for bone regeneration, ε-poly-l-lysine (EPL), an antimicrobic cationic polypeptide, was used for surface modification of 3D printed polycaprolactone/hydroxyapatite (PCL/HA) scaffolds which were fabricated by fused deposition modeling (FDM) technology. The scaffold morphology and micro-structure were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and transform infrared spectroscopy (FT-IR). The release profile surface roughness, open porosity, and mechanical properties of the scaffolds were evaluated. Cell adhesion, proliferation, differentiation potential and antibacterial properties were also examined. As a result, 3D printed PCL/HA scaffolds with interconnected pores showed a slightly rough surface and improved mechanical properties due to adding hydroxyapatite (HA) particles. After being modified by EPL, favorable biocompatibility and osteoconductivity of ε-poly-l-lysine/polycaprolactone/hydroxyapatite (EPL/PCL/HA) scaffolds were observed. Moreover, antibacterial activity of the EPL/PCL/HA scaffolds was apparent. As a consequence, the EPL/PCL/HA scaffolds had great potential for bone regeneration and prevention of infections. This would yield a patient-specific bioactive and antibacterial composite scaffold for advanced bone tissue engineering applications.
format Online
Article
Text
id pubmed-9049012
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-90490122022-04-28 Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds Tian, Lijiao Zhang, Zhenting Tian, Bin Zhang, Xin Wang, Na RSC Adv Chemistry Biomaterial scaffolds play a critical role in bone tissue engineering. Moreover, 3D printing technology has enormous advantage in the manufacture of bioengineering scaffolds for patient-specific bone defect treatments. In order to provide an aseptic environment for bone regeneration, ε-poly-l-lysine (EPL), an antimicrobic cationic polypeptide, was used for surface modification of 3D printed polycaprolactone/hydroxyapatite (PCL/HA) scaffolds which were fabricated by fused deposition modeling (FDM) technology. The scaffold morphology and micro-structure were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and transform infrared spectroscopy (FT-IR). The release profile surface roughness, open porosity, and mechanical properties of the scaffolds were evaluated. Cell adhesion, proliferation, differentiation potential and antibacterial properties were also examined. As a result, 3D printed PCL/HA scaffolds with interconnected pores showed a slightly rough surface and improved mechanical properties due to adding hydroxyapatite (HA) particles. After being modified by EPL, favorable biocompatibility and osteoconductivity of ε-poly-l-lysine/polycaprolactone/hydroxyapatite (EPL/PCL/HA) scaffolds were observed. Moreover, antibacterial activity of the EPL/PCL/HA scaffolds was apparent. As a consequence, the EPL/PCL/HA scaffolds had great potential for bone regeneration and prevention of infections. This would yield a patient-specific bioactive and antibacterial composite scaffold for advanced bone tissue engineering applications. The Royal Society of Chemistry 2020-01-29 /pmc/articles/PMC9049012/ /pubmed/35495239 http://dx.doi.org/10.1039/c9ra10275b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Tian, Lijiao
Zhang, Zhenting
Tian, Bin
Zhang, Xin
Wang, Na
Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds
title Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds
title_full Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds
title_fullStr Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds
title_full_unstemmed Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds
title_short Study on antibacterial properties and cytocompatibility of EPL coated 3D printed PCL/HA composite scaffolds
title_sort study on antibacterial properties and cytocompatibility of epl coated 3d printed pcl/ha composite scaffolds
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049012/
https://www.ncbi.nlm.nih.gov/pubmed/35495239
http://dx.doi.org/10.1039/c9ra10275b
work_keys_str_mv AT tianlijiao studyonantibacterialpropertiesandcytocompatibilityofeplcoated3dprintedpclhacompositescaffolds
AT zhangzhenting studyonantibacterialpropertiesandcytocompatibilityofeplcoated3dprintedpclhacompositescaffolds
AT tianbin studyonantibacterialpropertiesandcytocompatibilityofeplcoated3dprintedpclhacompositescaffolds
AT zhangxin studyonantibacterialpropertiesandcytocompatibilityofeplcoated3dprintedpclhacompositescaffolds
AT wangna studyonantibacterialpropertiesandcytocompatibilityofeplcoated3dprintedpclhacompositescaffolds