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The variability of nuclear DNA content of different Pelargonium species estimated by flow cytometry
Pelargonium is a versatile genus mainly from the Cape Region, South Africa. The genus is divided into four subgenera and 16 sections characterized by several groups of chromosomes sizes and numbers. The DNA content of species from all subgenera and sections of Pelargonium, except for the sections Su...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049363/ https://www.ncbi.nlm.nih.gov/pubmed/35482804 http://dx.doi.org/10.1371/journal.pone.0267496 |
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author | Plaschil, Sylvia Abel, Simone Klocke, Evelyn |
author_facet | Plaschil, Sylvia Abel, Simone Klocke, Evelyn |
author_sort | Plaschil, Sylvia |
collection | PubMed |
description | Pelargonium is a versatile genus mainly from the Cape Region, South Africa. The genus is divided into four subgenera and 16 sections characterized by several groups of chromosomes sizes and numbers. The DNA content of species from all subgenera and sections of Pelargonium, except for the sections Subsucculentia and Campylia was estimated using flow cytometry. Nuclei of Pelargonium samples (leaf or petal tissue) and an internal plant standard (leaf tissue) were isolated together and stained with propidium iodide. The DNA content was estimated providing that the 2C peaks of sample and standard be in linearity in the flow cytometer histograms. In total, 96 Pelargonium accessions of 60 species (22 Pelargonium species for the first time) were analyzed. The 2C DNA content ranged from 0.84 pg (P. longifolium, section Hoarea) to 6.69 pg (P. schizopetalum, section Magnistipulacea) and the corresponding 1Cx DNA content from 0.42 pg (P. longifolium) to 1.72 pg (P. transvaalense. This demonstrates the high plasticity within the genus Pelargonium. Some species, such as P. peltatum accessions revealed a pronounced endopolyploidization in leaves but not in petals underlining the importance to choose the right tissue as sample for the flow cytometry analysis. The reported genome sizes are a step forward towards the characterization of the Pelargonium collection within the German Gene Bank for Ornamental Plants and a valuable base for future sequencing programs of the Pelargonium genomes. |
format | Online Article Text |
id | pubmed-9049363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-90493632022-04-29 The variability of nuclear DNA content of different Pelargonium species estimated by flow cytometry Plaschil, Sylvia Abel, Simone Klocke, Evelyn PLoS One Research Article Pelargonium is a versatile genus mainly from the Cape Region, South Africa. The genus is divided into four subgenera and 16 sections characterized by several groups of chromosomes sizes and numbers. The DNA content of species from all subgenera and sections of Pelargonium, except for the sections Subsucculentia and Campylia was estimated using flow cytometry. Nuclei of Pelargonium samples (leaf or petal tissue) and an internal plant standard (leaf tissue) were isolated together and stained with propidium iodide. The DNA content was estimated providing that the 2C peaks of sample and standard be in linearity in the flow cytometer histograms. In total, 96 Pelargonium accessions of 60 species (22 Pelargonium species for the first time) were analyzed. The 2C DNA content ranged from 0.84 pg (P. longifolium, section Hoarea) to 6.69 pg (P. schizopetalum, section Magnistipulacea) and the corresponding 1Cx DNA content from 0.42 pg (P. longifolium) to 1.72 pg (P. transvaalense. This demonstrates the high plasticity within the genus Pelargonium. Some species, such as P. peltatum accessions revealed a pronounced endopolyploidization in leaves but not in petals underlining the importance to choose the right tissue as sample for the flow cytometry analysis. The reported genome sizes are a step forward towards the characterization of the Pelargonium collection within the German Gene Bank for Ornamental Plants and a valuable base for future sequencing programs of the Pelargonium genomes. Public Library of Science 2022-04-28 /pmc/articles/PMC9049363/ /pubmed/35482804 http://dx.doi.org/10.1371/journal.pone.0267496 Text en © 2022 Plaschil et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Plaschil, Sylvia Abel, Simone Klocke, Evelyn The variability of nuclear DNA content of different Pelargonium species estimated by flow cytometry |
title | The variability of nuclear DNA content of different Pelargonium species estimated by flow cytometry |
title_full | The variability of nuclear DNA content of different Pelargonium species estimated by flow cytometry |
title_fullStr | The variability of nuclear DNA content of different Pelargonium species estimated by flow cytometry |
title_full_unstemmed | The variability of nuclear DNA content of different Pelargonium species estimated by flow cytometry |
title_short | The variability of nuclear DNA content of different Pelargonium species estimated by flow cytometry |
title_sort | variability of nuclear dna content of different pelargonium species estimated by flow cytometry |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049363/ https://www.ncbi.nlm.nih.gov/pubmed/35482804 http://dx.doi.org/10.1371/journal.pone.0267496 |
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