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A unimolecular DNA fluorescent probe for determination of copper ions based on click chemistry

A homogenous fluorescence method was constructed for Cu(2+) detection by employing DNA-templated click chemistry and exonuclease reaction. In this strategy, a dumbbell shaped DNA probe, which contained an alkyne group and an azide group at its ends, was designed as the template for the click chemist...

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Autores principales: Qin, Yingfeng, Li, Ming, Yang, Yingying, Gao, Zhiying, Zhang, Huaisheng, Zhao, Jingjin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049493/
https://www.ncbi.nlm.nih.gov/pubmed/35497443
http://dx.doi.org/10.1039/c9ra10174h
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author Qin, Yingfeng
Li, Ming
Yang, Yingying
Gao, Zhiying
Zhang, Huaisheng
Zhao, Jingjin
author_facet Qin, Yingfeng
Li, Ming
Yang, Yingying
Gao, Zhiying
Zhang, Huaisheng
Zhao, Jingjin
author_sort Qin, Yingfeng
collection PubMed
description A homogenous fluorescence method was constructed for Cu(2+) detection by employing DNA-templated click chemistry and exonuclease reaction. In this strategy, a dumbbell shaped DNA probe, which contained an alkyne group and an azide group at its ends, was designed as the template for the click chemistry reaction, and also the signal probe. In the absence of Cu(2+), the DNA probe was digested into small oligonucleotide fragments by exonuclease, resulting in a low fluorescence background. However, this DNA probe can be sealed at its two ends by Cu(2+)-induced click chemistry ligation in the presence of Cu(2+). This closed structure of DNA would remain stable after addition of exonuclease, and could then be stained by SYBR Green I. A strong fluorescence signal was observed, which was related to the concentration of Cu(2+). This assay showed high selectivity and reached the detection limit of 39 nM. Moreover, the proposed strategy exhibited satisfactory detection results in real complex sample analysis, and has promising application in environmental monitoring and food safety.
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spelling pubmed-90494932022-04-29 A unimolecular DNA fluorescent probe for determination of copper ions based on click chemistry Qin, Yingfeng Li, Ming Yang, Yingying Gao, Zhiying Zhang, Huaisheng Zhao, Jingjin RSC Adv Chemistry A homogenous fluorescence method was constructed for Cu(2+) detection by employing DNA-templated click chemistry and exonuclease reaction. In this strategy, a dumbbell shaped DNA probe, which contained an alkyne group and an azide group at its ends, was designed as the template for the click chemistry reaction, and also the signal probe. In the absence of Cu(2+), the DNA probe was digested into small oligonucleotide fragments by exonuclease, resulting in a low fluorescence background. However, this DNA probe can be sealed at its two ends by Cu(2+)-induced click chemistry ligation in the presence of Cu(2+). This closed structure of DNA would remain stable after addition of exonuclease, and could then be stained by SYBR Green I. A strong fluorescence signal was observed, which was related to the concentration of Cu(2+). This assay showed high selectivity and reached the detection limit of 39 nM. Moreover, the proposed strategy exhibited satisfactory detection results in real complex sample analysis, and has promising application in environmental monitoring and food safety. The Royal Society of Chemistry 2020-02-06 /pmc/articles/PMC9049493/ /pubmed/35497443 http://dx.doi.org/10.1039/c9ra10174h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Qin, Yingfeng
Li, Ming
Yang, Yingying
Gao, Zhiying
Zhang, Huaisheng
Zhao, Jingjin
A unimolecular DNA fluorescent probe for determination of copper ions based on click chemistry
title A unimolecular DNA fluorescent probe for determination of copper ions based on click chemistry
title_full A unimolecular DNA fluorescent probe for determination of copper ions based on click chemistry
title_fullStr A unimolecular DNA fluorescent probe for determination of copper ions based on click chemistry
title_full_unstemmed A unimolecular DNA fluorescent probe for determination of copper ions based on click chemistry
title_short A unimolecular DNA fluorescent probe for determination of copper ions based on click chemistry
title_sort unimolecular dna fluorescent probe for determination of copper ions based on click chemistry
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049493/
https://www.ncbi.nlm.nih.gov/pubmed/35497443
http://dx.doi.org/10.1039/c9ra10174h
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