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Electrochemical immunosensor based on AuBP@Pt nanostructure and AuPd-PDA nanozyme for ultrasensitive detection of APOE4

An ultrasensitive sandwich-type electrochemical immunosensor based on AuBP@Pt nanostructures and AuPd-PDA nanozyme was developed for the detection of apolipoprotein E4 (APOE4) which was an important risk factor for Alzheimer's disease (AD). In this work, gold nanobipyramid coated Pt (AuBP@Pt) n...

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Autores principales: Liu, Yibiao, He, Guangli, Liu, Huili, Yin, Hang, Gao, Fengli, Chen, Jian, Zhang, Shouren, Yang, Baocheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049899/
https://www.ncbi.nlm.nih.gov/pubmed/35492164
http://dx.doi.org/10.1039/d0ra00298d
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author Liu, Yibiao
He, Guangli
Liu, Huili
Yin, Hang
Gao, Fengli
Chen, Jian
Zhang, Shouren
Yang, Baocheng
author_facet Liu, Yibiao
He, Guangli
Liu, Huili
Yin, Hang
Gao, Fengli
Chen, Jian
Zhang, Shouren
Yang, Baocheng
author_sort Liu, Yibiao
collection PubMed
description An ultrasensitive sandwich-type electrochemical immunosensor based on AuBP@Pt nanostructures and AuPd-PDA nanozyme was developed for the detection of apolipoprotein E4 (APOE4) which was an important risk factor for Alzheimer's disease (AD). In this work, gold nanobipyramid coated Pt (AuBP@Pt) nanostructures were prepared and applied to electrochemical immunosensors as a substrate material. AuBP@Pt nanostructures have advantages of electrical conductivity and large electroactive area, which could greatly increase electron transfer rate. In previous work, we designed AuPd alloy modified polydopamine (AuPd-PDA) nanozyme which catalyzed the decomposition of hydrogen peroxide (H(2)O(2)). AuPd-PDA nanozyme was used to label detection antibody due to excellent catalytic capability and stability in this new paper. And the concentration of APOE4 could be detected quantitatively by variation for transient current. As a result, the electrochemical immunosensor based on AuBP@Pt and AuPd-PDA exhibited a wide linear range from 0.05 to 2000 ng mL(−1) and low detection limit of 15.4 pg mL(−1) (S/N = 3). Furthermore, the designed biosensor displayed good selectivity in phosphate buffer saline (PBS) buffer solution or commercial goat serum, which provided a promising tool for early diagnosis of AD.
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spelling pubmed-90498992022-04-29 Electrochemical immunosensor based on AuBP@Pt nanostructure and AuPd-PDA nanozyme for ultrasensitive detection of APOE4 Liu, Yibiao He, Guangli Liu, Huili Yin, Hang Gao, Fengli Chen, Jian Zhang, Shouren Yang, Baocheng RSC Adv Chemistry An ultrasensitive sandwich-type electrochemical immunosensor based on AuBP@Pt nanostructures and AuPd-PDA nanozyme was developed for the detection of apolipoprotein E4 (APOE4) which was an important risk factor for Alzheimer's disease (AD). In this work, gold nanobipyramid coated Pt (AuBP@Pt) nanostructures were prepared and applied to electrochemical immunosensors as a substrate material. AuBP@Pt nanostructures have advantages of electrical conductivity and large electroactive area, which could greatly increase electron transfer rate. In previous work, we designed AuPd alloy modified polydopamine (AuPd-PDA) nanozyme which catalyzed the decomposition of hydrogen peroxide (H(2)O(2)). AuPd-PDA nanozyme was used to label detection antibody due to excellent catalytic capability and stability in this new paper. And the concentration of APOE4 could be detected quantitatively by variation for transient current. As a result, the electrochemical immunosensor based on AuBP@Pt and AuPd-PDA exhibited a wide linear range from 0.05 to 2000 ng mL(−1) and low detection limit of 15.4 pg mL(−1) (S/N = 3). Furthermore, the designed biosensor displayed good selectivity in phosphate buffer saline (PBS) buffer solution or commercial goat serum, which provided a promising tool for early diagnosis of AD. The Royal Society of Chemistry 2020-02-24 /pmc/articles/PMC9049899/ /pubmed/35492164 http://dx.doi.org/10.1039/d0ra00298d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Liu, Yibiao
He, Guangli
Liu, Huili
Yin, Hang
Gao, Fengli
Chen, Jian
Zhang, Shouren
Yang, Baocheng
Electrochemical immunosensor based on AuBP@Pt nanostructure and AuPd-PDA nanozyme for ultrasensitive detection of APOE4
title Electrochemical immunosensor based on AuBP@Pt nanostructure and AuPd-PDA nanozyme for ultrasensitive detection of APOE4
title_full Electrochemical immunosensor based on AuBP@Pt nanostructure and AuPd-PDA nanozyme for ultrasensitive detection of APOE4
title_fullStr Electrochemical immunosensor based on AuBP@Pt nanostructure and AuPd-PDA nanozyme for ultrasensitive detection of APOE4
title_full_unstemmed Electrochemical immunosensor based on AuBP@Pt nanostructure and AuPd-PDA nanozyme for ultrasensitive detection of APOE4
title_short Electrochemical immunosensor based on AuBP@Pt nanostructure and AuPd-PDA nanozyme for ultrasensitive detection of APOE4
title_sort electrochemical immunosensor based on aubp@pt nanostructure and aupd-pda nanozyme for ultrasensitive detection of apoe4
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049899/
https://www.ncbi.nlm.nih.gov/pubmed/35492164
http://dx.doi.org/10.1039/d0ra00298d
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