A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer

A colorimetric assay is developed for detection of lncRNA HOTTIP by one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) coupled with positively-charged gold nanoparticles ((+)AuNP) for diagnosis of pancreatic cancer. This assay allows simple, rapid, and sensitive quantifi...

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Detalles Bibliográficos
Autores principales: Lou, Ut Kei, Wong, Chi Hin, Chen, Yangchao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049936/
https://www.ncbi.nlm.nih.gov/pubmed/35497850
http://dx.doi.org/10.1039/c9ra07858d
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author Lou, Ut Kei
Wong, Chi Hin
Chen, Yangchao
author_facet Lou, Ut Kei
Wong, Chi Hin
Chen, Yangchao
author_sort Lou, Ut Kei
collection PubMed
description A colorimetric assay is developed for detection of lncRNA HOTTIP by one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) coupled with positively-charged gold nanoparticles ((+)AuNP) for diagnosis of pancreatic cancer. This assay allows simple, rapid, and sensitive quantification of lncRNA down to 50 copies.
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spelling pubmed-90499362022-04-29 A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer Lou, Ut Kei Wong, Chi Hin Chen, Yangchao RSC Adv Chemistry A colorimetric assay is developed for detection of lncRNA HOTTIP by one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) coupled with positively-charged gold nanoparticles ((+)AuNP) for diagnosis of pancreatic cancer. This assay allows simple, rapid, and sensitive quantification of lncRNA down to 50 copies. The Royal Society of Chemistry 2020-02-25 /pmc/articles/PMC9049936/ /pubmed/35497850 http://dx.doi.org/10.1039/c9ra07858d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Lou, Ut Kei
Wong, Chi Hin
Chen, Yangchao
A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer
title A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer
title_full A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer
title_fullStr A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer
title_full_unstemmed A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer
title_short A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer
title_sort simple and rapid colorimetric detection of serum lncrna biomarkers for diagnosis of pancreatic cancer
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049936/
https://www.ncbi.nlm.nih.gov/pubmed/35497850
http://dx.doi.org/10.1039/c9ra07858d
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