Cargando…
Kidney epithelial cells are active mechano-biological fluid pumps
The role of mechanical forces driving kidney epithelial fluid transport and morphogenesis in kidney diseases is unclear. Here, using a microfluidic platform to recapitulate fluid transport activity of kidney cells, we report that renal epithelial cells can actively generate hydraulic pressure gradie...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9050750/ https://www.ncbi.nlm.nih.gov/pubmed/35484146 http://dx.doi.org/10.1038/s41467-022-29988-w |
_version_ | 1784696438459990016 |
---|---|
author | Choudhury, Mohammad Ikbal Li, Yizeng Mistriotis, Panagiotis Vasconcelos, Ana Carina N. Dixon, Eryn E. Yang, Jing Benson, Morgan Maity, Debonil Walker, Rebecca Martin, Leigha Koroma, Fatima Qian, Feng Konstantopoulos, Konstantinos Woodward, Owen M. Sun, Sean X. |
author_facet | Choudhury, Mohammad Ikbal Li, Yizeng Mistriotis, Panagiotis Vasconcelos, Ana Carina N. Dixon, Eryn E. Yang, Jing Benson, Morgan Maity, Debonil Walker, Rebecca Martin, Leigha Koroma, Fatima Qian, Feng Konstantopoulos, Konstantinos Woodward, Owen M. Sun, Sean X. |
author_sort | Choudhury, Mohammad Ikbal |
collection | PubMed |
description | The role of mechanical forces driving kidney epithelial fluid transport and morphogenesis in kidney diseases is unclear. Here, using a microfluidic platform to recapitulate fluid transport activity of kidney cells, we report that renal epithelial cells can actively generate hydraulic pressure gradients across the epithelium. The fluidic flux declines with increasing hydraulic pressure until a stall pressure, in a manner similar to mechanical fluid pumps. For normal human kidney cells, the fluidic flux is from apical to basal, and the pressure is higher on the basal side. For human Autosomal Dominant Polycystic Kidney Disease cells, the fluidic flux is reversed from basal to apical. Molecular and proteomic studies reveal that renal epithelial cells are sensitive to hydraulic pressure gradients, changing gene expression profiles and spatial arrangements of ion exchangers and the cytoskeleton in different pressure conditions. These results implicate mechanical force and hydraulic pressure as important variables during kidney function and morphological change, and provide insights into pathophysiological mechanisms underlying the development and transduction of hydraulic pressure gradients. |
format | Online Article Text |
id | pubmed-9050750 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90507502022-04-30 Kidney epithelial cells are active mechano-biological fluid pumps Choudhury, Mohammad Ikbal Li, Yizeng Mistriotis, Panagiotis Vasconcelos, Ana Carina N. Dixon, Eryn E. Yang, Jing Benson, Morgan Maity, Debonil Walker, Rebecca Martin, Leigha Koroma, Fatima Qian, Feng Konstantopoulos, Konstantinos Woodward, Owen M. Sun, Sean X. Nat Commun Article The role of mechanical forces driving kidney epithelial fluid transport and morphogenesis in kidney diseases is unclear. Here, using a microfluidic platform to recapitulate fluid transport activity of kidney cells, we report that renal epithelial cells can actively generate hydraulic pressure gradients across the epithelium. The fluidic flux declines with increasing hydraulic pressure until a stall pressure, in a manner similar to mechanical fluid pumps. For normal human kidney cells, the fluidic flux is from apical to basal, and the pressure is higher on the basal side. For human Autosomal Dominant Polycystic Kidney Disease cells, the fluidic flux is reversed from basal to apical. Molecular and proteomic studies reveal that renal epithelial cells are sensitive to hydraulic pressure gradients, changing gene expression profiles and spatial arrangements of ion exchangers and the cytoskeleton in different pressure conditions. These results implicate mechanical force and hydraulic pressure as important variables during kidney function and morphological change, and provide insights into pathophysiological mechanisms underlying the development and transduction of hydraulic pressure gradients. Nature Publishing Group UK 2022-04-28 /pmc/articles/PMC9050750/ /pubmed/35484146 http://dx.doi.org/10.1038/s41467-022-29988-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Choudhury, Mohammad Ikbal Li, Yizeng Mistriotis, Panagiotis Vasconcelos, Ana Carina N. Dixon, Eryn E. Yang, Jing Benson, Morgan Maity, Debonil Walker, Rebecca Martin, Leigha Koroma, Fatima Qian, Feng Konstantopoulos, Konstantinos Woodward, Owen M. Sun, Sean X. Kidney epithelial cells are active mechano-biological fluid pumps |
title | Kidney epithelial cells are active mechano-biological fluid pumps |
title_full | Kidney epithelial cells are active mechano-biological fluid pumps |
title_fullStr | Kidney epithelial cells are active mechano-biological fluid pumps |
title_full_unstemmed | Kidney epithelial cells are active mechano-biological fluid pumps |
title_short | Kidney epithelial cells are active mechano-biological fluid pumps |
title_sort | kidney epithelial cells are active mechano-biological fluid pumps |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9050750/ https://www.ncbi.nlm.nih.gov/pubmed/35484146 http://dx.doi.org/10.1038/s41467-022-29988-w |
work_keys_str_mv | AT choudhurymohammadikbal kidneyepithelialcellsareactivemechanobiologicalfluidpumps AT liyizeng kidneyepithelialcellsareactivemechanobiologicalfluidpumps AT mistriotispanagiotis kidneyepithelialcellsareactivemechanobiologicalfluidpumps AT vasconcelosanacarinan kidneyepithelialcellsareactivemechanobiologicalfluidpumps AT dixoneryne kidneyepithelialcellsareactivemechanobiologicalfluidpumps AT yangjing kidneyepithelialcellsareactivemechanobiologicalfluidpumps AT bensonmorgan kidneyepithelialcellsareactivemechanobiologicalfluidpumps AT maitydebonil kidneyepithelialcellsareactivemechanobiologicalfluidpumps AT walkerrebecca kidneyepithelialcellsareactivemechanobiologicalfluidpumps AT martinleigha kidneyepithelialcellsareactivemechanobiologicalfluidpumps AT koromafatima kidneyepithelialcellsareactivemechanobiologicalfluidpumps AT qianfeng kidneyepithelialcellsareactivemechanobiologicalfluidpumps AT konstantopouloskonstantinos kidneyepithelialcellsareactivemechanobiologicalfluidpumps AT woodwardowenm kidneyepithelialcellsareactivemechanobiologicalfluidpumps AT sunseanx kidneyepithelialcellsareactivemechanobiologicalfluidpumps |