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Acoustofluidics for simultaneous nanoparticle-based drug loading and exosome encapsulation

Nanocarrier and exosome encapsulation has been found to significantly increase the efficacy of targeted drug delivery while also minimizing unwanted side effects. However, the development of exosome-encapsulated drug nanocarriers is limited by low drug loading efficiencies and/or complex, time-consu...

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Autores principales: Wang, Zeyu, Rich, Joseph, Hao, Nanjing, Gu, Yuyang, Chen, Chuyi, Yang, Shujie, Zhang, Peiran, Huang, Tony Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051122/
https://www.ncbi.nlm.nih.gov/pubmed/35498337
http://dx.doi.org/10.1038/s41378-022-00374-2
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author Wang, Zeyu
Rich, Joseph
Hao, Nanjing
Gu, Yuyang
Chen, Chuyi
Yang, Shujie
Zhang, Peiran
Huang, Tony Jun
author_facet Wang, Zeyu
Rich, Joseph
Hao, Nanjing
Gu, Yuyang
Chen, Chuyi
Yang, Shujie
Zhang, Peiran
Huang, Tony Jun
author_sort Wang, Zeyu
collection PubMed
description Nanocarrier and exosome encapsulation has been found to significantly increase the efficacy of targeted drug delivery while also minimizing unwanted side effects. However, the development of exosome-encapsulated drug nanocarriers is limited by low drug loading efficiencies and/or complex, time-consuming drug loading processes. Herein, we have developed an acoustofluidic device that simultaneously performs both drug loading and exosome encapsulation. By synergistically leveraging the acoustic radiation force, acoustic microstreaming, and shear stresses in a rotating droplet, the concentration, and fusion of exosomes, drugs, and porous silica nanoparticles is achieved. The final product consists of drug-loaded silica nanocarriers that are encased within an exosomal membrane. The drug loading efficiency is significantly improved, with nearly 30% of the free drug (e.g., doxorubicin) molecules loaded into the nanocarriers. Furthermore, this acoustofluidic drug loading system circumvents the need for complex chemical modification, allowing drug loading and encapsulation to be completed within a matter of minutes. These exosome-encapsulated nanocarriers exhibit excellent efficiency in intracellular transport and are capable of significantly inhibiting tumor cell proliferation. By utilizing physical forces to rapidly generate hybrid nanocarriers, this acoustofluidic drug loading platform wields the potential to significantly impact innovation in both drug delivery research and applications.
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spelling pubmed-90511222022-04-30 Acoustofluidics for simultaneous nanoparticle-based drug loading and exosome encapsulation Wang, Zeyu Rich, Joseph Hao, Nanjing Gu, Yuyang Chen, Chuyi Yang, Shujie Zhang, Peiran Huang, Tony Jun Microsyst Nanoeng Article Nanocarrier and exosome encapsulation has been found to significantly increase the efficacy of targeted drug delivery while also minimizing unwanted side effects. However, the development of exosome-encapsulated drug nanocarriers is limited by low drug loading efficiencies and/or complex, time-consuming drug loading processes. Herein, we have developed an acoustofluidic device that simultaneously performs both drug loading and exosome encapsulation. By synergistically leveraging the acoustic radiation force, acoustic microstreaming, and shear stresses in a rotating droplet, the concentration, and fusion of exosomes, drugs, and porous silica nanoparticles is achieved. The final product consists of drug-loaded silica nanocarriers that are encased within an exosomal membrane. The drug loading efficiency is significantly improved, with nearly 30% of the free drug (e.g., doxorubicin) molecules loaded into the nanocarriers. Furthermore, this acoustofluidic drug loading system circumvents the need for complex chemical modification, allowing drug loading and encapsulation to be completed within a matter of minutes. These exosome-encapsulated nanocarriers exhibit excellent efficiency in intracellular transport and are capable of significantly inhibiting tumor cell proliferation. By utilizing physical forces to rapidly generate hybrid nanocarriers, this acoustofluidic drug loading platform wields the potential to significantly impact innovation in both drug delivery research and applications. Nature Publishing Group UK 2022-04-28 /pmc/articles/PMC9051122/ /pubmed/35498337 http://dx.doi.org/10.1038/s41378-022-00374-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wang, Zeyu
Rich, Joseph
Hao, Nanjing
Gu, Yuyang
Chen, Chuyi
Yang, Shujie
Zhang, Peiran
Huang, Tony Jun
Acoustofluidics for simultaneous nanoparticle-based drug loading and exosome encapsulation
title Acoustofluidics for simultaneous nanoparticle-based drug loading and exosome encapsulation
title_full Acoustofluidics for simultaneous nanoparticle-based drug loading and exosome encapsulation
title_fullStr Acoustofluidics for simultaneous nanoparticle-based drug loading and exosome encapsulation
title_full_unstemmed Acoustofluidics for simultaneous nanoparticle-based drug loading and exosome encapsulation
title_short Acoustofluidics for simultaneous nanoparticle-based drug loading and exosome encapsulation
title_sort acoustofluidics for simultaneous nanoparticle-based drug loading and exosome encapsulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051122/
https://www.ncbi.nlm.nih.gov/pubmed/35498337
http://dx.doi.org/10.1038/s41378-022-00374-2
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