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Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study
Biomarkers of cell stress are important for proper diagnosis, and in studies of how cells respond to drug treatment. Biomarkers that respond early to pharmacological treatment could improve therapy by tailoring the treatment to the needs of the patient. Thymosin beta-4 (Tβ(4)) plays a significant ro...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051466/ https://www.ncbi.nlm.nih.gov/pubmed/35497634 http://dx.doi.org/10.1039/c9ra10365a |
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author | Coni, Pierpaolo Piras, Monica Mateddu, Anna Piludu, Marco Orru, Germano Scano, Alessandra Cabras, Tiziana Piras, Valentina Lachowicz, Joanna Izabela Jaremko, Mariusz Faa, Gavino Castagnola, Massimo Pichiri, Giuseppina |
author_facet | Coni, Pierpaolo Piras, Monica Mateddu, Anna Piludu, Marco Orru, Germano Scano, Alessandra Cabras, Tiziana Piras, Valentina Lachowicz, Joanna Izabela Jaremko, Mariusz Faa, Gavino Castagnola, Massimo Pichiri, Giuseppina |
author_sort | Coni, Pierpaolo |
collection | PubMed |
description | Biomarkers of cell stress are important for proper diagnosis, and in studies of how cells respond to drug treatment. Biomarkers that respond early to pharmacological treatment could improve therapy by tailoring the treatment to the needs of the patient. Thymosin beta-4 (Tβ(4)) plays a significant role in many aspects of cellular metabolism because of its actin-sequestering properties. Other physiological functions of Tβ(4) have been also reported. Among these, Tβ(4) may play a crucial role during cellular stress. We addressed the relevance of Tβ(4) in cellular stress conditions by using different treatments (serum starvation, DMSO, and butyrate administration) in a colon adenocarcinoma cell line (CaCo2), a cell line frequently used for in vitro experimental studies of Tβ(4). In this study, different stress stimuli were analyzed and the obtained results were compared using immunocytochemistry, and molecular and biochemical methods. Taken together, the data clearly indicate that the Tβ(4) peptide is involved in adaptive and defensive cellular mechanisms, and that different stress inducers lead to a similar Tβ(4) cytoplasmic/nuclear translocation. The translocation of Tβ(4) between the cytoplasm and the nucleus of the cell seems characteristic of a possible molecular response to cellular stress exerted by this peptide. |
format | Online Article Text |
id | pubmed-9051466 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90514662022-04-29 Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study Coni, Pierpaolo Piras, Monica Mateddu, Anna Piludu, Marco Orru, Germano Scano, Alessandra Cabras, Tiziana Piras, Valentina Lachowicz, Joanna Izabela Jaremko, Mariusz Faa, Gavino Castagnola, Massimo Pichiri, Giuseppina RSC Adv Chemistry Biomarkers of cell stress are important for proper diagnosis, and in studies of how cells respond to drug treatment. Biomarkers that respond early to pharmacological treatment could improve therapy by tailoring the treatment to the needs of the patient. Thymosin beta-4 (Tβ(4)) plays a significant role in many aspects of cellular metabolism because of its actin-sequestering properties. Other physiological functions of Tβ(4) have been also reported. Among these, Tβ(4) may play a crucial role during cellular stress. We addressed the relevance of Tβ(4) in cellular stress conditions by using different treatments (serum starvation, DMSO, and butyrate administration) in a colon adenocarcinoma cell line (CaCo2), a cell line frequently used for in vitro experimental studies of Tβ(4). In this study, different stress stimuli were analyzed and the obtained results were compared using immunocytochemistry, and molecular and biochemical methods. Taken together, the data clearly indicate that the Tβ(4) peptide is involved in adaptive and defensive cellular mechanisms, and that different stress inducers lead to a similar Tβ(4) cytoplasmic/nuclear translocation. The translocation of Tβ(4) between the cytoplasm and the nucleus of the cell seems characteristic of a possible molecular response to cellular stress exerted by this peptide. The Royal Society of Chemistry 2020-03-30 /pmc/articles/PMC9051466/ /pubmed/35497634 http://dx.doi.org/10.1039/c9ra10365a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Coni, Pierpaolo Piras, Monica Mateddu, Anna Piludu, Marco Orru, Germano Scano, Alessandra Cabras, Tiziana Piras, Valentina Lachowicz, Joanna Izabela Jaremko, Mariusz Faa, Gavino Castagnola, Massimo Pichiri, Giuseppina Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study |
title | Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study |
title_full | Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study |
title_fullStr | Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study |
title_full_unstemmed | Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study |
title_short | Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study |
title_sort | thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. a caco2 case study |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051466/ https://www.ncbi.nlm.nih.gov/pubmed/35497634 http://dx.doi.org/10.1039/c9ra10365a |
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