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Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study

Biomarkers of cell stress are important for proper diagnosis, and in studies of how cells respond to drug treatment. Biomarkers that respond early to pharmacological treatment could improve therapy by tailoring the treatment to the needs of the patient. Thymosin beta-4 (Tβ(4)) plays a significant ro...

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Autores principales: Coni, Pierpaolo, Piras, Monica, Mateddu, Anna, Piludu, Marco, Orru, Germano, Scano, Alessandra, Cabras, Tiziana, Piras, Valentina, Lachowicz, Joanna Izabela, Jaremko, Mariusz, Faa, Gavino, Castagnola, Massimo, Pichiri, Giuseppina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051466/
https://www.ncbi.nlm.nih.gov/pubmed/35497634
http://dx.doi.org/10.1039/c9ra10365a
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author Coni, Pierpaolo
Piras, Monica
Mateddu, Anna
Piludu, Marco
Orru, Germano
Scano, Alessandra
Cabras, Tiziana
Piras, Valentina
Lachowicz, Joanna Izabela
Jaremko, Mariusz
Faa, Gavino
Castagnola, Massimo
Pichiri, Giuseppina
author_facet Coni, Pierpaolo
Piras, Monica
Mateddu, Anna
Piludu, Marco
Orru, Germano
Scano, Alessandra
Cabras, Tiziana
Piras, Valentina
Lachowicz, Joanna Izabela
Jaremko, Mariusz
Faa, Gavino
Castagnola, Massimo
Pichiri, Giuseppina
author_sort Coni, Pierpaolo
collection PubMed
description Biomarkers of cell stress are important for proper diagnosis, and in studies of how cells respond to drug treatment. Biomarkers that respond early to pharmacological treatment could improve therapy by tailoring the treatment to the needs of the patient. Thymosin beta-4 (Tβ(4)) plays a significant role in many aspects of cellular metabolism because of its actin-sequestering properties. Other physiological functions of Tβ(4) have been also reported. Among these, Tβ(4) may play a crucial role during cellular stress. We addressed the relevance of Tβ(4) in cellular stress conditions by using different treatments (serum starvation, DMSO, and butyrate administration) in a colon adenocarcinoma cell line (CaCo2), a cell line frequently used for in vitro experimental studies of Tβ(4). In this study, different stress stimuli were analyzed and the obtained results were compared using immunocytochemistry, and molecular and biochemical methods. Taken together, the data clearly indicate that the Tβ(4) peptide is involved in adaptive and defensive cellular mechanisms, and that different stress inducers lead to a similar Tβ(4) cytoplasmic/nuclear translocation. The translocation of Tβ(4) between the cytoplasm and the nucleus of the cell seems characteristic of a possible molecular response to cellular stress exerted by this peptide.
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spelling pubmed-90514662022-04-29 Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study Coni, Pierpaolo Piras, Monica Mateddu, Anna Piludu, Marco Orru, Germano Scano, Alessandra Cabras, Tiziana Piras, Valentina Lachowicz, Joanna Izabela Jaremko, Mariusz Faa, Gavino Castagnola, Massimo Pichiri, Giuseppina RSC Adv Chemistry Biomarkers of cell stress are important for proper diagnosis, and in studies of how cells respond to drug treatment. Biomarkers that respond early to pharmacological treatment could improve therapy by tailoring the treatment to the needs of the patient. Thymosin beta-4 (Tβ(4)) plays a significant role in many aspects of cellular metabolism because of its actin-sequestering properties. Other physiological functions of Tβ(4) have been also reported. Among these, Tβ(4) may play a crucial role during cellular stress. We addressed the relevance of Tβ(4) in cellular stress conditions by using different treatments (serum starvation, DMSO, and butyrate administration) in a colon adenocarcinoma cell line (CaCo2), a cell line frequently used for in vitro experimental studies of Tβ(4). In this study, different stress stimuli were analyzed and the obtained results were compared using immunocytochemistry, and molecular and biochemical methods. Taken together, the data clearly indicate that the Tβ(4) peptide is involved in adaptive and defensive cellular mechanisms, and that different stress inducers lead to a similar Tβ(4) cytoplasmic/nuclear translocation. The translocation of Tβ(4) between the cytoplasm and the nucleus of the cell seems characteristic of a possible molecular response to cellular stress exerted by this peptide. The Royal Society of Chemistry 2020-03-30 /pmc/articles/PMC9051466/ /pubmed/35497634 http://dx.doi.org/10.1039/c9ra10365a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Coni, Pierpaolo
Piras, Monica
Mateddu, Anna
Piludu, Marco
Orru, Germano
Scano, Alessandra
Cabras, Tiziana
Piras, Valentina
Lachowicz, Joanna Izabela
Jaremko, Mariusz
Faa, Gavino
Castagnola, Massimo
Pichiri, Giuseppina
Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study
title Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study
title_full Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study
title_fullStr Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study
title_full_unstemmed Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study
title_short Thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. A Caco2 case study
title_sort thymosin β4 cytoplasmic/nuclear translocation as a new marker of cellular stress. a caco2 case study
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051466/
https://www.ncbi.nlm.nih.gov/pubmed/35497634
http://dx.doi.org/10.1039/c9ra10365a
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