Bead based facile assay for sensitive quantification of native state green fluorescent protein

A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-...

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Detalles Bibliográficos
Autores principales: Kim, Jung Min, Seong, Baik Lin, Lim, Dong-Kwon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051470/
https://www.ncbi.nlm.nih.gov/pubmed/35492102
http://dx.doi.org/10.1039/c9ra09599c
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author Kim, Jung Min
Seong, Baik Lin
Lim, Dong-Kwon
author_facet Kim, Jung Min
Seong, Baik Lin
Lim, Dong-Kwon
author_sort Kim, Jung Min
collection PubMed
description A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-nitrilotriacetic acid)-modified microbead particles. The bead-based method is simple and straightforward to perform and it showed a highly sensitive capability to detect the expressed fluorescent protein because of the enriched fluorescent protein on the beads.
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spelling pubmed-90514702022-04-29 Bead based facile assay for sensitive quantification of native state green fluorescent protein Kim, Jung Min Seong, Baik Lin Lim, Dong-Kwon RSC Adv Chemistry A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-nitrilotriacetic acid)-modified microbead particles. The bead-based method is simple and straightforward to perform and it showed a highly sensitive capability to detect the expressed fluorescent protein because of the enriched fluorescent protein on the beads. The Royal Society of Chemistry 2020-04-01 /pmc/articles/PMC9051470/ /pubmed/35492102 http://dx.doi.org/10.1039/c9ra09599c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Kim, Jung Min
Seong, Baik Lin
Lim, Dong-Kwon
Bead based facile assay for sensitive quantification of native state green fluorescent protein
title Bead based facile assay for sensitive quantification of native state green fluorescent protein
title_full Bead based facile assay for sensitive quantification of native state green fluorescent protein
title_fullStr Bead based facile assay for sensitive quantification of native state green fluorescent protein
title_full_unstemmed Bead based facile assay for sensitive quantification of native state green fluorescent protein
title_short Bead based facile assay for sensitive quantification of native state green fluorescent protein
title_sort bead based facile assay for sensitive quantification of native state green fluorescent protein
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051470/
https://www.ncbi.nlm.nih.gov/pubmed/35492102
http://dx.doi.org/10.1039/c9ra09599c
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AT limdongkwon beadbasedfacileassayforsensitivequantificationofnativestategreenfluorescentprotein

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