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Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock
Grafting has become a common practice for watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai] production in many parts of the world, due to its efficacy against biotic and abiotic stressors. However, grafting success for watermelon is challenging in part due to the complex anatomy of the cucu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051512/ https://www.ncbi.nlm.nih.gov/pubmed/35498692 http://dx.doi.org/10.3389/fpls.2022.878289 |
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author | Devi, Pinki DeVetter, Lisa Kraft, Michael Shrestha, Srijana Miles, Carol |
author_facet | Devi, Pinki DeVetter, Lisa Kraft, Michael Shrestha, Srijana Miles, Carol |
author_sort | Devi, Pinki |
collection | PubMed |
description | Grafting has become a common practice for watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai] production in many parts of the world, due to its efficacy against biotic and abiotic stressors. However, grafting success for watermelon is challenging in part due to the complex anatomy of the cucurbit vascular system. The survival of grafted transplants depends on compatibility between the scion and rootstock, which in turn depends on anatomical, physiological, and genetic variables. A better understanding of cucurbit anatomy and graft union formation would inform grafting approaches and transplant management. An anatomical study was conducted by scanning electron microscopy (SEM) at 11 and 25 days after grafting (DAG) with seedless watermelon cultivar ‘Secretariat’ grafted onto compatible rootstock cultivars ‘Pelop’ (Lagenaria siceraria) and ‘Tetsukabuto’ (Cucurbita maxima × Cucurbita moschata) in comparison to non-grafted watermelon and rootstock seedlings. At 11 DAG, the parenchymatic cells of the central pith of grafted plants were dead and a necrotic layer was observed, representing the beginning of callus formation. New xylem strands were formed in the vascular system, connecting the rootstock with the scion. At 25 DAG, fully developed vascular bundles at the graft interface were observed with both scion-rootstock combinations. Although more studies are necessary to characterize the sequence of physiological events after grafting in Cucurbit species, this is one of the first studies to describe the complex anatomical changes that occur during watermelon graft healing. |
format | Online Article Text |
id | pubmed-9051512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90515122022-04-30 Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock Devi, Pinki DeVetter, Lisa Kraft, Michael Shrestha, Srijana Miles, Carol Front Plant Sci Plant Science Grafting has become a common practice for watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai] production in many parts of the world, due to its efficacy against biotic and abiotic stressors. However, grafting success for watermelon is challenging in part due to the complex anatomy of the cucurbit vascular system. The survival of grafted transplants depends on compatibility between the scion and rootstock, which in turn depends on anatomical, physiological, and genetic variables. A better understanding of cucurbit anatomy and graft union formation would inform grafting approaches and transplant management. An anatomical study was conducted by scanning electron microscopy (SEM) at 11 and 25 days after grafting (DAG) with seedless watermelon cultivar ‘Secretariat’ grafted onto compatible rootstock cultivars ‘Pelop’ (Lagenaria siceraria) and ‘Tetsukabuto’ (Cucurbita maxima × Cucurbita moschata) in comparison to non-grafted watermelon and rootstock seedlings. At 11 DAG, the parenchymatic cells of the central pith of grafted plants were dead and a necrotic layer was observed, representing the beginning of callus formation. New xylem strands were formed in the vascular system, connecting the rootstock with the scion. At 25 DAG, fully developed vascular bundles at the graft interface were observed with both scion-rootstock combinations. Although more studies are necessary to characterize the sequence of physiological events after grafting in Cucurbit species, this is one of the first studies to describe the complex anatomical changes that occur during watermelon graft healing. Frontiers Media S.A. 2022-04-15 /pmc/articles/PMC9051512/ /pubmed/35498692 http://dx.doi.org/10.3389/fpls.2022.878289 Text en Copyright © 2022 Devi, DeVetter, Kraft, Shrestha and Miles. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Devi, Pinki DeVetter, Lisa Kraft, Michael Shrestha, Srijana Miles, Carol Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock |
title | Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock |
title_full | Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock |
title_fullStr | Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock |
title_full_unstemmed | Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock |
title_short | Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock |
title_sort | micrographic view of graft union formation between watermelon scion and squash rootstock |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051512/ https://www.ncbi.nlm.nih.gov/pubmed/35498692 http://dx.doi.org/10.3389/fpls.2022.878289 |
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