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Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock

Grafting has become a common practice for watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai] production in many parts of the world, due to its efficacy against biotic and abiotic stressors. However, grafting success for watermelon is challenging in part due to the complex anatomy of the cucu...

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Autores principales: Devi, Pinki, DeVetter, Lisa, Kraft, Michael, Shrestha, Srijana, Miles, Carol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051512/
https://www.ncbi.nlm.nih.gov/pubmed/35498692
http://dx.doi.org/10.3389/fpls.2022.878289
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author Devi, Pinki
DeVetter, Lisa
Kraft, Michael
Shrestha, Srijana
Miles, Carol
author_facet Devi, Pinki
DeVetter, Lisa
Kraft, Michael
Shrestha, Srijana
Miles, Carol
author_sort Devi, Pinki
collection PubMed
description Grafting has become a common practice for watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai] production in many parts of the world, due to its efficacy against biotic and abiotic stressors. However, grafting success for watermelon is challenging in part due to the complex anatomy of the cucurbit vascular system. The survival of grafted transplants depends on compatibility between the scion and rootstock, which in turn depends on anatomical, physiological, and genetic variables. A better understanding of cucurbit anatomy and graft union formation would inform grafting approaches and transplant management. An anatomical study was conducted by scanning electron microscopy (SEM) at 11 and 25 days after grafting (DAG) with seedless watermelon cultivar ‘Secretariat’ grafted onto compatible rootstock cultivars ‘Pelop’ (Lagenaria siceraria) and ‘Tetsukabuto’ (Cucurbita maxima × Cucurbita moschata) in comparison to non-grafted watermelon and rootstock seedlings. At 11 DAG, the parenchymatic cells of the central pith of grafted plants were dead and a necrotic layer was observed, representing the beginning of callus formation. New xylem strands were formed in the vascular system, connecting the rootstock with the scion. At 25 DAG, fully developed vascular bundles at the graft interface were observed with both scion-rootstock combinations. Although more studies are necessary to characterize the sequence of physiological events after grafting in Cucurbit species, this is one of the first studies to describe the complex anatomical changes that occur during watermelon graft healing.
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spelling pubmed-90515122022-04-30 Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock Devi, Pinki DeVetter, Lisa Kraft, Michael Shrestha, Srijana Miles, Carol Front Plant Sci Plant Science Grafting has become a common practice for watermelon [Citrullus lanatus (Thunb.) Matsum & Nakai] production in many parts of the world, due to its efficacy against biotic and abiotic stressors. However, grafting success for watermelon is challenging in part due to the complex anatomy of the cucurbit vascular system. The survival of grafted transplants depends on compatibility between the scion and rootstock, which in turn depends on anatomical, physiological, and genetic variables. A better understanding of cucurbit anatomy and graft union formation would inform grafting approaches and transplant management. An anatomical study was conducted by scanning electron microscopy (SEM) at 11 and 25 days after grafting (DAG) with seedless watermelon cultivar ‘Secretariat’ grafted onto compatible rootstock cultivars ‘Pelop’ (Lagenaria siceraria) and ‘Tetsukabuto’ (Cucurbita maxima × Cucurbita moschata) in comparison to non-grafted watermelon and rootstock seedlings. At 11 DAG, the parenchymatic cells of the central pith of grafted plants were dead and a necrotic layer was observed, representing the beginning of callus formation. New xylem strands were formed in the vascular system, connecting the rootstock with the scion. At 25 DAG, fully developed vascular bundles at the graft interface were observed with both scion-rootstock combinations. Although more studies are necessary to characterize the sequence of physiological events after grafting in Cucurbit species, this is one of the first studies to describe the complex anatomical changes that occur during watermelon graft healing. Frontiers Media S.A. 2022-04-15 /pmc/articles/PMC9051512/ /pubmed/35498692 http://dx.doi.org/10.3389/fpls.2022.878289 Text en Copyright © 2022 Devi, DeVetter, Kraft, Shrestha and Miles. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Devi, Pinki
DeVetter, Lisa
Kraft, Michael
Shrestha, Srijana
Miles, Carol
Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock
title Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock
title_full Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock
title_fullStr Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock
title_full_unstemmed Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock
title_short Micrographic View of Graft Union Formation Between Watermelon Scion and Squash Rootstock
title_sort micrographic view of graft union formation between watermelon scion and squash rootstock
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051512/
https://www.ncbi.nlm.nih.gov/pubmed/35498692
http://dx.doi.org/10.3389/fpls.2022.878289
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