The C-Terminal Transmembrane Domain of Cowpea Mild Mottle Virus TGBp2 Is Critical for Plasmodesmata Localization and for Its Interaction With TGBp1 and TGBp3

The movement of some plant RNA viruses is mediated by triple gene block (TGB) proteins, which cooperate to transfer the viral genome from cell to cell through plasmodesmata. Here, we investigated the function of the TGB proteins of cowpea mild mottle virus (CPMMV; genus Carlavirus, family Betaflexiv...

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Autores principales: Jiang, Chong, Shan, Shiqi, Huang, Yue, Mao, Chenyang, Zhang, Hehong, Li, Yanjun, Chen, Jianping, Wei, Zhongyan, Sun, Zongtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051516/
https://www.ncbi.nlm.nih.gov/pubmed/35495691
http://dx.doi.org/10.3389/fmicb.2022.860695
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author Jiang, Chong
Shan, Shiqi
Huang, Yue
Mao, Chenyang
Zhang, Hehong
Li, Yanjun
Chen, Jianping
Wei, Zhongyan
Sun, Zongtao
author_facet Jiang, Chong
Shan, Shiqi
Huang, Yue
Mao, Chenyang
Zhang, Hehong
Li, Yanjun
Chen, Jianping
Wei, Zhongyan
Sun, Zongtao
author_sort Jiang, Chong
collection PubMed
description The movement of some plant RNA viruses is mediated by triple gene block (TGB) proteins, which cooperate to transfer the viral genome from cell to cell through plasmodesmata. Here, we investigated the function of the TGB proteins of cowpea mild mottle virus (CPMMV; genus Carlavirus, family Betaflexiviridae), which causes severe damage to soybean production. Subcellular localization experiments demonstrated that TGBp1 and TGBp3 were localized to the endoplasmic reticulum (ER), plasmodesmata (PD) and nucleus in Nicotiana benthamiana leaves. TGBp2 was unusually localized to PD. In protein interaction assays TGBp2 significantly enhanced the interaction between TGBp3 and TGBp1. Interaction assays using deletion mutants showed that the C-terminal transmembrane (TM) domain of TGBp2 is critical for its localization to PD and for its interaction with TGBp1 and TGBp3.
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spelling pubmed-90515162022-04-30 The C-Terminal Transmembrane Domain of Cowpea Mild Mottle Virus TGBp2 Is Critical for Plasmodesmata Localization and for Its Interaction With TGBp1 and TGBp3 Jiang, Chong Shan, Shiqi Huang, Yue Mao, Chenyang Zhang, Hehong Li, Yanjun Chen, Jianping Wei, Zhongyan Sun, Zongtao Front Microbiol Microbiology The movement of some plant RNA viruses is mediated by triple gene block (TGB) proteins, which cooperate to transfer the viral genome from cell to cell through plasmodesmata. Here, we investigated the function of the TGB proteins of cowpea mild mottle virus (CPMMV; genus Carlavirus, family Betaflexiviridae), which causes severe damage to soybean production. Subcellular localization experiments demonstrated that TGBp1 and TGBp3 were localized to the endoplasmic reticulum (ER), plasmodesmata (PD) and nucleus in Nicotiana benthamiana leaves. TGBp2 was unusually localized to PD. In protein interaction assays TGBp2 significantly enhanced the interaction between TGBp3 and TGBp1. Interaction assays using deletion mutants showed that the C-terminal transmembrane (TM) domain of TGBp2 is critical for its localization to PD and for its interaction with TGBp1 and TGBp3. Frontiers Media S.A. 2022-04-15 /pmc/articles/PMC9051516/ /pubmed/35495691 http://dx.doi.org/10.3389/fmicb.2022.860695 Text en Copyright © 2022 Jiang, Shan, Huang, Mao, Zhang, Li, Chen, Wei and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Jiang, Chong
Shan, Shiqi
Huang, Yue
Mao, Chenyang
Zhang, Hehong
Li, Yanjun
Chen, Jianping
Wei, Zhongyan
Sun, Zongtao
The C-Terminal Transmembrane Domain of Cowpea Mild Mottle Virus TGBp2 Is Critical for Plasmodesmata Localization and for Its Interaction With TGBp1 and TGBp3
title The C-Terminal Transmembrane Domain of Cowpea Mild Mottle Virus TGBp2 Is Critical for Plasmodesmata Localization and for Its Interaction With TGBp1 and TGBp3
title_full The C-Terminal Transmembrane Domain of Cowpea Mild Mottle Virus TGBp2 Is Critical for Plasmodesmata Localization and for Its Interaction With TGBp1 and TGBp3
title_fullStr The C-Terminal Transmembrane Domain of Cowpea Mild Mottle Virus TGBp2 Is Critical for Plasmodesmata Localization and for Its Interaction With TGBp1 and TGBp3
title_full_unstemmed The C-Terminal Transmembrane Domain of Cowpea Mild Mottle Virus TGBp2 Is Critical for Plasmodesmata Localization and for Its Interaction With TGBp1 and TGBp3
title_short The C-Terminal Transmembrane Domain of Cowpea Mild Mottle Virus TGBp2 Is Critical for Plasmodesmata Localization and for Its Interaction With TGBp1 and TGBp3
title_sort c-terminal transmembrane domain of cowpea mild mottle virus tgbp2 is critical for plasmodesmata localization and for its interaction with tgbp1 and tgbp3
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051516/
https://www.ncbi.nlm.nih.gov/pubmed/35495691
http://dx.doi.org/10.3389/fmicb.2022.860695
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