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Carbon and Nitrogen Metabolism Are Jointly Regulated During Shading in Roots and Leaves of Camellia Sinensis

Numerous studies have shown that plant shading can promote the quality of green tea. However, the association of shading with metabolic regulation in tea leaves and roots remains unelucidated. Here, the metabolic profiling of two tea cultivars (“Xiangfeicui” and “Jinxuan”) in response to shading and...

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Autores principales: Shao, Chenyu, Jiao, Haizhen, Chen, Jiahao, Zhang, Chenyu, Liu, Jie, Chen, Jianjiao, Li, Yunfei, Huang, Jing, Yang, Biao, Liu, Zhonghua, Shen, Chengwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051521/
https://www.ncbi.nlm.nih.gov/pubmed/35498711
http://dx.doi.org/10.3389/fpls.2022.894840
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author Shao, Chenyu
Jiao, Haizhen
Chen, Jiahao
Zhang, Chenyu
Liu, Jie
Chen, Jianjiao
Li, Yunfei
Huang, Jing
Yang, Biao
Liu, Zhonghua
Shen, Chengwen
author_facet Shao, Chenyu
Jiao, Haizhen
Chen, Jiahao
Zhang, Chenyu
Liu, Jie
Chen, Jianjiao
Li, Yunfei
Huang, Jing
Yang, Biao
Liu, Zhonghua
Shen, Chengwen
author_sort Shao, Chenyu
collection PubMed
description Numerous studies have shown that plant shading can promote the quality of green tea. However, the association of shading with metabolic regulation in tea leaves and roots remains unelucidated. Here, the metabolic profiling of two tea cultivars (“Xiangfeicui” and “Jinxuan”) in response to shading and relighting periods during the summer season was performed using non-targeted metabolomics methods. The metabolic pathway analyses revealed that long-term shading remarkably inhibit the sugar metabolism such as glycolysis, galactose metabolism, and pentose phosphate pathway in the leaves and roots of “Xiangfeicui,” and “Jinxuan” were more sensitive to light recovery changes. The lipid metabolism in the leaves and roots of “Xiangfeicui” was promoted by short-term shading, while it was inhibited by long-term shading. In addition, the intensity of the flavonoid metabolites in the leaves and roots of “Jinxuan” were upregulated with a trend of rising first and then decreasing under shading, and five flavonoid synthesis genes showed the same trend (F3H, F3′5′H, DFR, ANS, and ANR). Simultaneously, the amino acids of the nitrogen metabolism in the leaves and roots of the two cultivars were significantly promoted by long-term shading, while the purine and caffeine metabolism was inhibited in the leaves of “Xiangfeicui.” Interestingly, CsGS1.1 and CsTSI, amino acid synthase genes was upregulated in the leaves and roots of two cultivars. These results indicated that shading could participate in carbon and nitrogen metabolic regulation of both leaf and root, and root metabolism could have a positive association with leaf metabolism to promote the shaded tea quality.
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spelling pubmed-90515212022-04-30 Carbon and Nitrogen Metabolism Are Jointly Regulated During Shading in Roots and Leaves of Camellia Sinensis Shao, Chenyu Jiao, Haizhen Chen, Jiahao Zhang, Chenyu Liu, Jie Chen, Jianjiao Li, Yunfei Huang, Jing Yang, Biao Liu, Zhonghua Shen, Chengwen Front Plant Sci Plant Science Numerous studies have shown that plant shading can promote the quality of green tea. However, the association of shading with metabolic regulation in tea leaves and roots remains unelucidated. Here, the metabolic profiling of two tea cultivars (“Xiangfeicui” and “Jinxuan”) in response to shading and relighting periods during the summer season was performed using non-targeted metabolomics methods. The metabolic pathway analyses revealed that long-term shading remarkably inhibit the sugar metabolism such as glycolysis, galactose metabolism, and pentose phosphate pathway in the leaves and roots of “Xiangfeicui,” and “Jinxuan” were more sensitive to light recovery changes. The lipid metabolism in the leaves and roots of “Xiangfeicui” was promoted by short-term shading, while it was inhibited by long-term shading. In addition, the intensity of the flavonoid metabolites in the leaves and roots of “Jinxuan” were upregulated with a trend of rising first and then decreasing under shading, and five flavonoid synthesis genes showed the same trend (F3H, F3′5′H, DFR, ANS, and ANR). Simultaneously, the amino acids of the nitrogen metabolism in the leaves and roots of the two cultivars were significantly promoted by long-term shading, while the purine and caffeine metabolism was inhibited in the leaves of “Xiangfeicui.” Interestingly, CsGS1.1 and CsTSI, amino acid synthase genes was upregulated in the leaves and roots of two cultivars. These results indicated that shading could participate in carbon and nitrogen metabolic regulation of both leaf and root, and root metabolism could have a positive association with leaf metabolism to promote the shaded tea quality. Frontiers Media S.A. 2022-04-15 /pmc/articles/PMC9051521/ /pubmed/35498711 http://dx.doi.org/10.3389/fpls.2022.894840 Text en Copyright © 2022 Shao, Jiao, Chen, Zhang, Liu, Chen, Li, Huang, Yang, Liu and Shen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Shao, Chenyu
Jiao, Haizhen
Chen, Jiahao
Zhang, Chenyu
Liu, Jie
Chen, Jianjiao
Li, Yunfei
Huang, Jing
Yang, Biao
Liu, Zhonghua
Shen, Chengwen
Carbon and Nitrogen Metabolism Are Jointly Regulated During Shading in Roots and Leaves of Camellia Sinensis
title Carbon and Nitrogen Metabolism Are Jointly Regulated During Shading in Roots and Leaves of Camellia Sinensis
title_full Carbon and Nitrogen Metabolism Are Jointly Regulated During Shading in Roots and Leaves of Camellia Sinensis
title_fullStr Carbon and Nitrogen Metabolism Are Jointly Regulated During Shading in Roots and Leaves of Camellia Sinensis
title_full_unstemmed Carbon and Nitrogen Metabolism Are Jointly Regulated During Shading in Roots and Leaves of Camellia Sinensis
title_short Carbon and Nitrogen Metabolism Are Jointly Regulated During Shading in Roots and Leaves of Camellia Sinensis
title_sort carbon and nitrogen metabolism are jointly regulated during shading in roots and leaves of camellia sinensis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051521/
https://www.ncbi.nlm.nih.gov/pubmed/35498711
http://dx.doi.org/10.3389/fpls.2022.894840
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