Cargando…
The SUMO E3 Ligase MdSIZ1 Sumoylates a Cell Number Regulator MdCNR8 to Control Organ Size
Plant growth and organ size putatively associated with crop yield are regulated by a complex network of genes including ones for controlling cell proliferation. The gene fw2.2 was first identified in tomatoes and reported to govern fruit size variation through controlling cell division. In this stud...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051543/ https://www.ncbi.nlm.nih.gov/pubmed/35498700 http://dx.doi.org/10.3389/fpls.2022.836935 |
_version_ | 1784696582326714368 |
---|---|
author | Wang, Gui-Luan Zhang, Chun-Ling Huo, He-Qiang Sun, Xiao-Shuai Zhang, Ya-Li Hao, Yu-Jin You, Chun-Xiang |
author_facet | Wang, Gui-Luan Zhang, Chun-Ling Huo, He-Qiang Sun, Xiao-Shuai Zhang, Ya-Li Hao, Yu-Jin You, Chun-Xiang |
author_sort | Wang, Gui-Luan |
collection | PubMed |
description | Plant growth and organ size putatively associated with crop yield are regulated by a complex network of genes including ones for controlling cell proliferation. The gene fw2.2 was first identified in tomatoes and reported to govern fruit size variation through controlling cell division. In this study, we isolated a putative ortholog of the tomato fw2.2 gene from apple, Cell Number Regulator 8 (MdCNR8). Our functional analysis showed that MdCNR8 may control fruit size and root growth. MdCNR8 was mediated by the SUMO E3 ligase MdSIZ1, and SUMOylation of MdCNR8 at residue-Lys39 promoted the translocation of MdCNR8 from plasma membrane to the nucleus. The effect of MdCNR8 in inhibiting root elongation could be completely counteracted by the coexpression of MdSIZ1. Moreover, the lower cell proliferation of apple calli due to silencing MdSIZ1 could be rescued by silencing MdCNR8. Collectively, our results showed that the MdSIZ1-mediated SUMOylation is required for the fulfillment of MdCNR8 in regulating cell proliferation to control plant organ size. This regulatory interaction between MdSIZ1 and MdCNR8 will facilitate understanding the mechanism underlying the regulation of organ size. |
format | Online Article Text |
id | pubmed-9051543 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90515432022-04-30 The SUMO E3 Ligase MdSIZ1 Sumoylates a Cell Number Regulator MdCNR8 to Control Organ Size Wang, Gui-Luan Zhang, Chun-Ling Huo, He-Qiang Sun, Xiao-Shuai Zhang, Ya-Li Hao, Yu-Jin You, Chun-Xiang Front Plant Sci Plant Science Plant growth and organ size putatively associated with crop yield are regulated by a complex network of genes including ones for controlling cell proliferation. The gene fw2.2 was first identified in tomatoes and reported to govern fruit size variation through controlling cell division. In this study, we isolated a putative ortholog of the tomato fw2.2 gene from apple, Cell Number Regulator 8 (MdCNR8). Our functional analysis showed that MdCNR8 may control fruit size and root growth. MdCNR8 was mediated by the SUMO E3 ligase MdSIZ1, and SUMOylation of MdCNR8 at residue-Lys39 promoted the translocation of MdCNR8 from plasma membrane to the nucleus. The effect of MdCNR8 in inhibiting root elongation could be completely counteracted by the coexpression of MdSIZ1. Moreover, the lower cell proliferation of apple calli due to silencing MdSIZ1 could be rescued by silencing MdCNR8. Collectively, our results showed that the MdSIZ1-mediated SUMOylation is required for the fulfillment of MdCNR8 in regulating cell proliferation to control plant organ size. This regulatory interaction between MdSIZ1 and MdCNR8 will facilitate understanding the mechanism underlying the regulation of organ size. Frontiers Media S.A. 2022-04-15 /pmc/articles/PMC9051543/ /pubmed/35498700 http://dx.doi.org/10.3389/fpls.2022.836935 Text en Copyright © 2022 Wang, Zhang, Huo, Sun, Zhang, Hao and You. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Wang, Gui-Luan Zhang, Chun-Ling Huo, He-Qiang Sun, Xiao-Shuai Zhang, Ya-Li Hao, Yu-Jin You, Chun-Xiang The SUMO E3 Ligase MdSIZ1 Sumoylates a Cell Number Regulator MdCNR8 to Control Organ Size |
title | The SUMO E3 Ligase MdSIZ1 Sumoylates a Cell Number Regulator MdCNR8 to Control Organ Size |
title_full | The SUMO E3 Ligase MdSIZ1 Sumoylates a Cell Number Regulator MdCNR8 to Control Organ Size |
title_fullStr | The SUMO E3 Ligase MdSIZ1 Sumoylates a Cell Number Regulator MdCNR8 to Control Organ Size |
title_full_unstemmed | The SUMO E3 Ligase MdSIZ1 Sumoylates a Cell Number Regulator MdCNR8 to Control Organ Size |
title_short | The SUMO E3 Ligase MdSIZ1 Sumoylates a Cell Number Regulator MdCNR8 to Control Organ Size |
title_sort | sumo e3 ligase mdsiz1 sumoylates a cell number regulator mdcnr8 to control organ size |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9051543/ https://www.ncbi.nlm.nih.gov/pubmed/35498700 http://dx.doi.org/10.3389/fpls.2022.836935 |
work_keys_str_mv | AT wangguiluan thesumoe3ligasemdsiz1sumoylatesacellnumberregulatormdcnr8tocontrolorgansize AT zhangchunling thesumoe3ligasemdsiz1sumoylatesacellnumberregulatormdcnr8tocontrolorgansize AT huoheqiang thesumoe3ligasemdsiz1sumoylatesacellnumberregulatormdcnr8tocontrolorgansize AT sunxiaoshuai thesumoe3ligasemdsiz1sumoylatesacellnumberregulatormdcnr8tocontrolorgansize AT zhangyali thesumoe3ligasemdsiz1sumoylatesacellnumberregulatormdcnr8tocontrolorgansize AT haoyujin thesumoe3ligasemdsiz1sumoylatesacellnumberregulatormdcnr8tocontrolorgansize AT youchunxiang thesumoe3ligasemdsiz1sumoylatesacellnumberregulatormdcnr8tocontrolorgansize AT wangguiluan sumoe3ligasemdsiz1sumoylatesacellnumberregulatormdcnr8tocontrolorgansize AT zhangchunling sumoe3ligasemdsiz1sumoylatesacellnumberregulatormdcnr8tocontrolorgansize AT huoheqiang sumoe3ligasemdsiz1sumoylatesacellnumberregulatormdcnr8tocontrolorgansize AT sunxiaoshuai sumoe3ligasemdsiz1sumoylatesacellnumberregulatormdcnr8tocontrolorgansize AT zhangyali sumoe3ligasemdsiz1sumoylatesacellnumberregulatormdcnr8tocontrolorgansize AT haoyujin sumoe3ligasemdsiz1sumoylatesacellnumberregulatormdcnr8tocontrolorgansize AT youchunxiang sumoe3ligasemdsiz1sumoylatesacellnumberregulatormdcnr8tocontrolorgansize |