Cargando…

(GTG)(5)-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso

BACKGROUND: Escherichia coli (E. coli) is the most common bacterial species implicated in various types of infections including septicemia, gastroenteritis, urinary tract infections, meningitis and others pathologies. These involve several bacterial clones with multidrug resistance making them diffi...

Descripción completa

Detalles Bibliográficos
Autores principales: Kaboré, Boukaré, Ouédraogo, Ganamé Abasse, Cissé, Hama, Ouédraogo, Henri S., Sampo, Emmanuel, Zongo, Koudbi Jacob, Zeba, Boukaré, Traoré, Yves, Gnankiné, Olivier, Sanou, Idrissa, Savadogo, Aly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9052641/
https://www.ncbi.nlm.nih.gov/pubmed/35488211
http://dx.doi.org/10.1186/s12866-022-02537-7
_version_ 1784696825512460288
author Kaboré, Boukaré
Ouédraogo, Ganamé Abasse
Cissé, Hama
Ouédraogo, Henri S.
Sampo, Emmanuel
Zongo, Koudbi Jacob
Zeba, Boukaré
Traoré, Yves
Gnankiné, Olivier
Sanou, Idrissa
Savadogo, Aly
author_facet Kaboré, Boukaré
Ouédraogo, Ganamé Abasse
Cissé, Hama
Ouédraogo, Henri S.
Sampo, Emmanuel
Zongo, Koudbi Jacob
Zeba, Boukaré
Traoré, Yves
Gnankiné, Olivier
Sanou, Idrissa
Savadogo, Aly
author_sort Kaboré, Boukaré
collection PubMed
description BACKGROUND: Escherichia coli (E. coli) is the most common bacterial species implicated in various types of infections including septicemia, gastroenteritis, urinary tract infections, meningitis and others pathologies. These involve several bacterial clones with multidrug resistance making them difficult to treat. The aims of this study was to perform molecular typing of E. coli strains using universal primer (GTG)(5). In this study, 53 E. coli strains were collected from inpatients and outpatients. The test of antimicrobial sensibility was realized using CA-SFM /EUCAST method and strains were identified by conventional microbiological tests. The carbapenemase-producing strains were demonstrated by phenotypic method. Bacterial DNA was extracted by boiling method. (GTG)(5)-PCR was used for strain subtyping. The DendroUPGMA software was used for grouping of strains from the genetic fingerprints obtained by (GTG)(5)-PCR. RESULTS: Antibiotic susceptibility test revealed that all strains were multi-drug resistant (MDR). Its strains showed resistance to at least three different families of antibiotics. Of this MDR strains, only one was a metallo-β-lactamase producer. The dendrogram obtained using genetic fingerprinting allowed the E. coli strains to be grouped into 22 clusters (G1 to G22). CONCLUSION: The (GTG) 5-PCR assay enabled rapid molecular typing of E. coli strains. The strains of E. coli typed in this study would belong to different clones. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02537-7.
format Online
Article
Text
id pubmed-9052641
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-90526412022-04-30 (GTG)(5)-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso Kaboré, Boukaré Ouédraogo, Ganamé Abasse Cissé, Hama Ouédraogo, Henri S. Sampo, Emmanuel Zongo, Koudbi Jacob Zeba, Boukaré Traoré, Yves Gnankiné, Olivier Sanou, Idrissa Savadogo, Aly BMC Microbiol Research BACKGROUND: Escherichia coli (E. coli) is the most common bacterial species implicated in various types of infections including septicemia, gastroenteritis, urinary tract infections, meningitis and others pathologies. These involve several bacterial clones with multidrug resistance making them difficult to treat. The aims of this study was to perform molecular typing of E. coli strains using universal primer (GTG)(5). In this study, 53 E. coli strains were collected from inpatients and outpatients. The test of antimicrobial sensibility was realized using CA-SFM /EUCAST method and strains were identified by conventional microbiological tests. The carbapenemase-producing strains were demonstrated by phenotypic method. Bacterial DNA was extracted by boiling method. (GTG)(5)-PCR was used for strain subtyping. The DendroUPGMA software was used for grouping of strains from the genetic fingerprints obtained by (GTG)(5)-PCR. RESULTS: Antibiotic susceptibility test revealed that all strains were multi-drug resistant (MDR). Its strains showed resistance to at least three different families of antibiotics. Of this MDR strains, only one was a metallo-β-lactamase producer. The dendrogram obtained using genetic fingerprinting allowed the E. coli strains to be grouped into 22 clusters (G1 to G22). CONCLUSION: The (GTG) 5-PCR assay enabled rapid molecular typing of E. coli strains. The strains of E. coli typed in this study would belong to different clones. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02537-7. BioMed Central 2022-04-29 /pmc/articles/PMC9052641/ /pubmed/35488211 http://dx.doi.org/10.1186/s12866-022-02537-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kaboré, Boukaré
Ouédraogo, Ganamé Abasse
Cissé, Hama
Ouédraogo, Henri S.
Sampo, Emmanuel
Zongo, Koudbi Jacob
Zeba, Boukaré
Traoré, Yves
Gnankiné, Olivier
Sanou, Idrissa
Savadogo, Aly
(GTG)(5)-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso
title (GTG)(5)-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso
title_full (GTG)(5)-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso
title_fullStr (GTG)(5)-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso
title_full_unstemmed (GTG)(5)-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso
title_short (GTG)(5)-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso
title_sort (gtg)(5)-pcr fingerprinting of multi-drug resistant escherichia coli bacteria isolates from hospital in ouagadougou, burkina faso
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9052641/
https://www.ncbi.nlm.nih.gov/pubmed/35488211
http://dx.doi.org/10.1186/s12866-022-02537-7
work_keys_str_mv AT kaboreboukare gtg5pcrfingerprintingofmultidrugresistantescherichiacolibacteriaisolatesfromhospitalinouagadougouburkinafaso
AT ouedraogoganameabasse gtg5pcrfingerprintingofmultidrugresistantescherichiacolibacteriaisolatesfromhospitalinouagadougouburkinafaso
AT cissehama gtg5pcrfingerprintingofmultidrugresistantescherichiacolibacteriaisolatesfromhospitalinouagadougouburkinafaso
AT ouedraogohenris gtg5pcrfingerprintingofmultidrugresistantescherichiacolibacteriaisolatesfromhospitalinouagadougouburkinafaso
AT sampoemmanuel gtg5pcrfingerprintingofmultidrugresistantescherichiacolibacteriaisolatesfromhospitalinouagadougouburkinafaso
AT zongokoudbijacob gtg5pcrfingerprintingofmultidrugresistantescherichiacolibacteriaisolatesfromhospitalinouagadougouburkinafaso
AT zebaboukare gtg5pcrfingerprintingofmultidrugresistantescherichiacolibacteriaisolatesfromhospitalinouagadougouburkinafaso
AT traoreyves gtg5pcrfingerprintingofmultidrugresistantescherichiacolibacteriaisolatesfromhospitalinouagadougouburkinafaso
AT gnankineolivier gtg5pcrfingerprintingofmultidrugresistantescherichiacolibacteriaisolatesfromhospitalinouagadougouburkinafaso
AT sanouidrissa gtg5pcrfingerprintingofmultidrugresistantescherichiacolibacteriaisolatesfromhospitalinouagadougouburkinafaso
AT savadogoaly gtg5pcrfingerprintingofmultidrugresistantescherichiacolibacteriaisolatesfromhospitalinouagadougouburkinafaso