Cargando…

Artificial Protein Cage with Unusual Geometry and Regularly Embedded Gold Nanoparticles

[Image: see text] Artificial protein cages have great potential in a number of areas including cargo capture and delivery and as artificial vaccines. Here, we investigate an artificial protein cage whose assembly is triggered by gold nanoparticles. Using biochemical and biophysical methods we were a...

Descripción completa

Detalles Bibliográficos
Autores principales: Majsterkiewicz, Karolina, Biela, Artur P., Maity, Sourav, Sharma, Mohit, Piette, Bernard M. A. G., Kowalczyk, Agnieszka, Gaweł, Szymon, Chakraborti, Soumyananda, Roos, Wouter H., Heddle, Jonathan G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9052746/
https://www.ncbi.nlm.nih.gov/pubmed/35254086
http://dx.doi.org/10.1021/acs.nanolett.1c04222
_version_ 1784696848432234496
author Majsterkiewicz, Karolina
Biela, Artur P.
Maity, Sourav
Sharma, Mohit
Piette, Bernard M. A. G.
Kowalczyk, Agnieszka
Gaweł, Szymon
Chakraborti, Soumyananda
Roos, Wouter H.
Heddle, Jonathan G.
author_facet Majsterkiewicz, Karolina
Biela, Artur P.
Maity, Sourav
Sharma, Mohit
Piette, Bernard M. A. G.
Kowalczyk, Agnieszka
Gaweł, Szymon
Chakraborti, Soumyananda
Roos, Wouter H.
Heddle, Jonathan G.
author_sort Majsterkiewicz, Karolina
collection PubMed
description [Image: see text] Artificial protein cages have great potential in a number of areas including cargo capture and delivery and as artificial vaccines. Here, we investigate an artificial protein cage whose assembly is triggered by gold nanoparticles. Using biochemical and biophysical methods we were able to determine both the mechanical properties and the gross compositional features of the cage which, combined with mathematical models and biophysical data, allowed the structure of the cage to be predicted. The accuracy of the overall geometrical prediction was confirmed by the cryo-EM structure determined to sub-5 Å resolution. This showed the cage to be nonregular but similar to a dodecahedron, being constructed from 12 11-membered rings. Surprisingly, the structure revealed that the cage also contained a single, small gold nanoparticle at each 3-fold axis meaning that each cage acts as a synthetic framework for regular arrangement of 20 gold nanoparticles in a three-dimensional lattice.
format Online
Article
Text
id pubmed-9052746
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-90527462022-05-02 Artificial Protein Cage with Unusual Geometry and Regularly Embedded Gold Nanoparticles Majsterkiewicz, Karolina Biela, Artur P. Maity, Sourav Sharma, Mohit Piette, Bernard M. A. G. Kowalczyk, Agnieszka Gaweł, Szymon Chakraborti, Soumyananda Roos, Wouter H. Heddle, Jonathan G. Nano Lett [Image: see text] Artificial protein cages have great potential in a number of areas including cargo capture and delivery and as artificial vaccines. Here, we investigate an artificial protein cage whose assembly is triggered by gold nanoparticles. Using biochemical and biophysical methods we were able to determine both the mechanical properties and the gross compositional features of the cage which, combined with mathematical models and biophysical data, allowed the structure of the cage to be predicted. The accuracy of the overall geometrical prediction was confirmed by the cryo-EM structure determined to sub-5 Å resolution. This showed the cage to be nonregular but similar to a dodecahedron, being constructed from 12 11-membered rings. Surprisingly, the structure revealed that the cage also contained a single, small gold nanoparticle at each 3-fold axis meaning that each cage acts as a synthetic framework for regular arrangement of 20 gold nanoparticles in a three-dimensional lattice. American Chemical Society 2022-03-07 2022-04-27 /pmc/articles/PMC9052746/ /pubmed/35254086 http://dx.doi.org/10.1021/acs.nanolett.1c04222 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Majsterkiewicz, Karolina
Biela, Artur P.
Maity, Sourav
Sharma, Mohit
Piette, Bernard M. A. G.
Kowalczyk, Agnieszka
Gaweł, Szymon
Chakraborti, Soumyananda
Roos, Wouter H.
Heddle, Jonathan G.
Artificial Protein Cage with Unusual Geometry and Regularly Embedded Gold Nanoparticles
title Artificial Protein Cage with Unusual Geometry and Regularly Embedded Gold Nanoparticles
title_full Artificial Protein Cage with Unusual Geometry and Regularly Embedded Gold Nanoparticles
title_fullStr Artificial Protein Cage with Unusual Geometry and Regularly Embedded Gold Nanoparticles
title_full_unstemmed Artificial Protein Cage with Unusual Geometry and Regularly Embedded Gold Nanoparticles
title_short Artificial Protein Cage with Unusual Geometry and Regularly Embedded Gold Nanoparticles
title_sort artificial protein cage with unusual geometry and regularly embedded gold nanoparticles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9052746/
https://www.ncbi.nlm.nih.gov/pubmed/35254086
http://dx.doi.org/10.1021/acs.nanolett.1c04222
work_keys_str_mv AT majsterkiewiczkarolina artificialproteincagewithunusualgeometryandregularlyembeddedgoldnanoparticles
AT bielaarturp artificialproteincagewithunusualgeometryandregularlyembeddedgoldnanoparticles
AT maitysourav artificialproteincagewithunusualgeometryandregularlyembeddedgoldnanoparticles
AT sharmamohit artificialproteincagewithunusualgeometryandregularlyembeddedgoldnanoparticles
AT piettebernardmag artificialproteincagewithunusualgeometryandregularlyembeddedgoldnanoparticles
AT kowalczykagnieszka artificialproteincagewithunusualgeometryandregularlyembeddedgoldnanoparticles
AT gawełszymon artificialproteincagewithunusualgeometryandregularlyembeddedgoldnanoparticles
AT chakrabortisoumyananda artificialproteincagewithunusualgeometryandregularlyembeddedgoldnanoparticles
AT rooswouterh artificialproteincagewithunusualgeometryandregularlyembeddedgoldnanoparticles
AT heddlejonathang artificialproteincagewithunusualgeometryandregularlyembeddedgoldnanoparticles