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Colloidal silver-based lateral flow immunoassay for detection of profenofos pesticide residue in vegetables

A colloidal silver nanoparticle (AgNP)-based lateral flow immunoassay (LFIA) was evaluated in terms of the rapid detection of profenofos (PEO) pesticide residue in vegetables. Colloidal AgNPs, of a diameter of approximately 20 nm, were surface-modified with trisodium citrate dehydrate (TSC) in order...

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Autores principales: Wu, Kuo-Hui, Huang, Wen-Chien, Chang, Shu-Chen, Shyu, Rong-Hwa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9052933/
https://www.ncbi.nlm.nih.gov/pubmed/35497005
http://dx.doi.org/10.1039/d2ra01654k
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author Wu, Kuo-Hui
Huang, Wen-Chien
Chang, Shu-Chen
Shyu, Rong-Hwa
author_facet Wu, Kuo-Hui
Huang, Wen-Chien
Chang, Shu-Chen
Shyu, Rong-Hwa
author_sort Wu, Kuo-Hui
collection PubMed
description A colloidal silver nanoparticle (AgNP)-based lateral flow immunoassay (LFIA) was evaluated in terms of the rapid detection of profenofos (PEO) pesticide residue in vegetables. Colloidal AgNPs, of a diameter of approximately 20 nm, were surface-modified with trisodium citrate dehydrate (TSC) in order to improve their stability and dispersion. An anti-profenofos polyclonal antibody (pAb) was successfully immobilized on the surface of the AgNPs by ionic interaction and characterized using UV-vis, SEM, TEM, FTIR and XPS analyses. Surface modification of Ag–pAb conjugates of varying pH, pAb content and cross-reactivity was employed to design and prepare labels for use in an LFIA to examine whether these factors affect the performance of the assay. The visible detection limit and optical detection limit of the PEO test strip were 0.20 and 0.01 ppm, respectively, in PEO standard solution. This assay showed no cross-reaction with omethoate, methamidophos or pyraclofos. Finally, the PEO test strip was effectively applied for the detection of PEO in liquid vegetables A and B, with optical detection limits of 0.09 and 0.075 ppm, respectively.
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spelling pubmed-90529332022-04-29 Colloidal silver-based lateral flow immunoassay for detection of profenofos pesticide residue in vegetables Wu, Kuo-Hui Huang, Wen-Chien Chang, Shu-Chen Shyu, Rong-Hwa RSC Adv Chemistry A colloidal silver nanoparticle (AgNP)-based lateral flow immunoassay (LFIA) was evaluated in terms of the rapid detection of profenofos (PEO) pesticide residue in vegetables. Colloidal AgNPs, of a diameter of approximately 20 nm, were surface-modified with trisodium citrate dehydrate (TSC) in order to improve their stability and dispersion. An anti-profenofos polyclonal antibody (pAb) was successfully immobilized on the surface of the AgNPs by ionic interaction and characterized using UV-vis, SEM, TEM, FTIR and XPS analyses. Surface modification of Ag–pAb conjugates of varying pH, pAb content and cross-reactivity was employed to design and prepare labels for use in an LFIA to examine whether these factors affect the performance of the assay. The visible detection limit and optical detection limit of the PEO test strip were 0.20 and 0.01 ppm, respectively, in PEO standard solution. This assay showed no cross-reaction with omethoate, methamidophos or pyraclofos. Finally, the PEO test strip was effectively applied for the detection of PEO in liquid vegetables A and B, with optical detection limits of 0.09 and 0.075 ppm, respectively. The Royal Society of Chemistry 2022-04-29 /pmc/articles/PMC9052933/ /pubmed/35497005 http://dx.doi.org/10.1039/d2ra01654k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Wu, Kuo-Hui
Huang, Wen-Chien
Chang, Shu-Chen
Shyu, Rong-Hwa
Colloidal silver-based lateral flow immunoassay for detection of profenofos pesticide residue in vegetables
title Colloidal silver-based lateral flow immunoassay for detection of profenofos pesticide residue in vegetables
title_full Colloidal silver-based lateral flow immunoassay for detection of profenofos pesticide residue in vegetables
title_fullStr Colloidal silver-based lateral flow immunoassay for detection of profenofos pesticide residue in vegetables
title_full_unstemmed Colloidal silver-based lateral flow immunoassay for detection of profenofos pesticide residue in vegetables
title_short Colloidal silver-based lateral flow immunoassay for detection of profenofos pesticide residue in vegetables
title_sort colloidal silver-based lateral flow immunoassay for detection of profenofos pesticide residue in vegetables
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9052933/
https://www.ncbi.nlm.nih.gov/pubmed/35497005
http://dx.doi.org/10.1039/d2ra01654k
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