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Dynamic crosslinked and injectable biohydrogels as extracellular matrix mimics for the delivery of antibiotics and 3D cell culture

Antibiotics are widely used in clinical medicine. As an important member, vancomycin often plays an irreplaceable role in some serious infections but for its use, there is still a lack of suitable carriers and effective formulations. To find a vancomycin carrier with potential for clinical applicati...

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Autores principales: Fan, Zhiping, Cheng, Ping, Liu, Min, Prakash, Sangeeta, Han, Jun, Ding, Zhuang, Zhao, Yanna, Wang, Zhengping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054094/
https://www.ncbi.nlm.nih.gov/pubmed/35515461
http://dx.doi.org/10.1039/d0ra02218g
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author Fan, Zhiping
Cheng, Ping
Liu, Min
Prakash, Sangeeta
Han, Jun
Ding, Zhuang
Zhao, Yanna
Wang, Zhengping
author_facet Fan, Zhiping
Cheng, Ping
Liu, Min
Prakash, Sangeeta
Han, Jun
Ding, Zhuang
Zhao, Yanna
Wang, Zhengping
author_sort Fan, Zhiping
collection PubMed
description Antibiotics are widely used in clinical medicine. As an important member, vancomycin often plays an irreplaceable role in some serious infections but for its use, there is still a lack of suitable carriers and effective formulations. To find a vancomycin carrier with potential for clinical applications, a new class of poly(γ-glutamic acid)/dextran-based injectable hydrogels have been constructed through dynamic covalent hydrazone linkages. Adipic dihydrazide (ADH)-grafted poly(γ-glutamic acid) (PGAADH) and sodium periodate-oxidized dextran (OD) precursors were synthesized; then, the hydrogels were formed by blending PGAADH and OD buffer solutions without any additives under physiological conditions. The newly formed precursor structures, mechanical properties, morphologies, hydrogel degradation profiles, and the interaction between the drug and precursors were investigated with FTIR spectroscopy, (1)H NMR spectroscopy, rheological experiments, compression tests, SEM, and isothermal titration calorimetric (ITC) measurements. The resulting hydrogels exhibited excellent antibacterial ability and ideal variable performances. Moreover, the hydrogels exhibited different drug release kinetics and mechanisms and were applied effectively towards the controlled release of vancomycin. Significantly, benefitting from the reversibly cross-linked systems and the excellent biocompatibility, the hydrogels can work as the ideal material for HeLa cell culture, leading to encapsulated cells with higher viability and capacity that is proliferative. Therefore, the injectable PGAADH/OD hydrogels demonstrated attractive properties for future applications in pharmaceutics and tissue engineering.
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spelling pubmed-90540942022-05-04 Dynamic crosslinked and injectable biohydrogels as extracellular matrix mimics for the delivery of antibiotics and 3D cell culture Fan, Zhiping Cheng, Ping Liu, Min Prakash, Sangeeta Han, Jun Ding, Zhuang Zhao, Yanna Wang, Zhengping RSC Adv Chemistry Antibiotics are widely used in clinical medicine. As an important member, vancomycin often plays an irreplaceable role in some serious infections but for its use, there is still a lack of suitable carriers and effective formulations. To find a vancomycin carrier with potential for clinical applications, a new class of poly(γ-glutamic acid)/dextran-based injectable hydrogels have been constructed through dynamic covalent hydrazone linkages. Adipic dihydrazide (ADH)-grafted poly(γ-glutamic acid) (PGAADH) and sodium periodate-oxidized dextran (OD) precursors were synthesized; then, the hydrogels were formed by blending PGAADH and OD buffer solutions without any additives under physiological conditions. The newly formed precursor structures, mechanical properties, morphologies, hydrogel degradation profiles, and the interaction between the drug and precursors were investigated with FTIR spectroscopy, (1)H NMR spectroscopy, rheological experiments, compression tests, SEM, and isothermal titration calorimetric (ITC) measurements. The resulting hydrogels exhibited excellent antibacterial ability and ideal variable performances. Moreover, the hydrogels exhibited different drug release kinetics and mechanisms and were applied effectively towards the controlled release of vancomycin. Significantly, benefitting from the reversibly cross-linked systems and the excellent biocompatibility, the hydrogels can work as the ideal material for HeLa cell culture, leading to encapsulated cells with higher viability and capacity that is proliferative. Therefore, the injectable PGAADH/OD hydrogels demonstrated attractive properties for future applications in pharmaceutics and tissue engineering. The Royal Society of Chemistry 2020-05-22 /pmc/articles/PMC9054094/ /pubmed/35515461 http://dx.doi.org/10.1039/d0ra02218g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Fan, Zhiping
Cheng, Ping
Liu, Min
Prakash, Sangeeta
Han, Jun
Ding, Zhuang
Zhao, Yanna
Wang, Zhengping
Dynamic crosslinked and injectable biohydrogels as extracellular matrix mimics for the delivery of antibiotics and 3D cell culture
title Dynamic crosslinked and injectable biohydrogels as extracellular matrix mimics for the delivery of antibiotics and 3D cell culture
title_full Dynamic crosslinked and injectable biohydrogels as extracellular matrix mimics for the delivery of antibiotics and 3D cell culture
title_fullStr Dynamic crosslinked and injectable biohydrogels as extracellular matrix mimics for the delivery of antibiotics and 3D cell culture
title_full_unstemmed Dynamic crosslinked and injectable biohydrogels as extracellular matrix mimics for the delivery of antibiotics and 3D cell culture
title_short Dynamic crosslinked and injectable biohydrogels as extracellular matrix mimics for the delivery of antibiotics and 3D cell culture
title_sort dynamic crosslinked and injectable biohydrogels as extracellular matrix mimics for the delivery of antibiotics and 3d cell culture
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054094/
https://www.ncbi.nlm.nih.gov/pubmed/35515461
http://dx.doi.org/10.1039/d0ra02218g
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