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Antileukaemic Cell Proliferation and Cytotoxic Activity of Edible Golden Cordyceps (Cordyceps militaris) Extracts

Golden cordyceps (Cordyceps militaris) is a mushroom of the genus Cordyceps. It has been used as a food supplement for both healthy and ill people. In this study, the antileukaemic cell proliferation activities of golden cordyceps extracts were examined and compared with standard cordycepin (CDCP) i...

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Autores principales: Tima, Singkome, Tapingkae, Tawat, To-anun, Chaiwat, Noireung, Parinn, Intaparn, Phikul, Chaiyana, Wantida, Sirithunyalug, Jakkapan, Panyajai, Pawaret, Viriyaadhammaa, Natsima, Nirachonkul, Wariya, Rueankham, Lapamas, Aung, Win Lae, Chueahongthong, Fah, Chiampanichayakul, Sawitree, Anuchapreeda, Songyot
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054435/
https://www.ncbi.nlm.nih.gov/pubmed/35497915
http://dx.doi.org/10.1155/2022/5347718
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author Tima, Singkome
Tapingkae, Tawat
To-anun, Chaiwat
Noireung, Parinn
Intaparn, Phikul
Chaiyana, Wantida
Sirithunyalug, Jakkapan
Panyajai, Pawaret
Viriyaadhammaa, Natsima
Nirachonkul, Wariya
Rueankham, Lapamas
Aung, Win Lae
Chueahongthong, Fah
Chiampanichayakul, Sawitree
Anuchapreeda, Songyot
author_facet Tima, Singkome
Tapingkae, Tawat
To-anun, Chaiwat
Noireung, Parinn
Intaparn, Phikul
Chaiyana, Wantida
Sirithunyalug, Jakkapan
Panyajai, Pawaret
Viriyaadhammaa, Natsima
Nirachonkul, Wariya
Rueankham, Lapamas
Aung, Win Lae
Chueahongthong, Fah
Chiampanichayakul, Sawitree
Anuchapreeda, Songyot
author_sort Tima, Singkome
collection PubMed
description Golden cordyceps (Cordyceps militaris) is a mushroom of the genus Cordyceps. It has been used as a food supplement for both healthy and ill people. In this study, the antileukaemic cell proliferation activities of golden cordyceps extracts were examined and compared with standard cordycepin (CDCP) in EoL-1, U937, and KG-1a cells. Wilms' tumour 1 (WT1) protein was used as a biomarker of leukaemic cell proliferation. The cytotoxicity of the extracts on leukaemic cells was determined using the MTT assay. Their inhibitory effects on WT1 protein expression and cell cycle progression of EoL-1 cells were investigated using Western blotting and flow cytometry, respectively. Induction of KG-1a cell differentiation (using CD11b as a marker) was determined using flow cytometry. The golden cordyceps extracts exhibited cytotoxic effects on leukaemic cells with the highest IC(50) value of 16.5 ± 3.9 µg/mL, while there was no effect on normal blood cells. The expression levels of WT1 protein in EoL-1 cells were decreased after treatment with the extracts. Moreover, cell cycle progression and cell proliferation were inhibited. The levels of CD11b increased slightly following the treatment. All these findings confirm the antileukaemic proliferation activity of golden cordyceps.
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spelling pubmed-90544352022-04-30 Antileukaemic Cell Proliferation and Cytotoxic Activity of Edible Golden Cordyceps (Cordyceps militaris) Extracts Tima, Singkome Tapingkae, Tawat To-anun, Chaiwat Noireung, Parinn Intaparn, Phikul Chaiyana, Wantida Sirithunyalug, Jakkapan Panyajai, Pawaret Viriyaadhammaa, Natsima Nirachonkul, Wariya Rueankham, Lapamas Aung, Win Lae Chueahongthong, Fah Chiampanichayakul, Sawitree Anuchapreeda, Songyot Evid Based Complement Alternat Med Research Article Golden cordyceps (Cordyceps militaris) is a mushroom of the genus Cordyceps. It has been used as a food supplement for both healthy and ill people. In this study, the antileukaemic cell proliferation activities of golden cordyceps extracts were examined and compared with standard cordycepin (CDCP) in EoL-1, U937, and KG-1a cells. Wilms' tumour 1 (WT1) protein was used as a biomarker of leukaemic cell proliferation. The cytotoxicity of the extracts on leukaemic cells was determined using the MTT assay. Their inhibitory effects on WT1 protein expression and cell cycle progression of EoL-1 cells were investigated using Western blotting and flow cytometry, respectively. Induction of KG-1a cell differentiation (using CD11b as a marker) was determined using flow cytometry. The golden cordyceps extracts exhibited cytotoxic effects on leukaemic cells with the highest IC(50) value of 16.5 ± 3.9 µg/mL, while there was no effect on normal blood cells. The expression levels of WT1 protein in EoL-1 cells were decreased after treatment with the extracts. Moreover, cell cycle progression and cell proliferation were inhibited. The levels of CD11b increased slightly following the treatment. All these findings confirm the antileukaemic proliferation activity of golden cordyceps. Hindawi 2022-04-22 /pmc/articles/PMC9054435/ /pubmed/35497915 http://dx.doi.org/10.1155/2022/5347718 Text en Copyright © 2022 Singkome Tima et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tima, Singkome
Tapingkae, Tawat
To-anun, Chaiwat
Noireung, Parinn
Intaparn, Phikul
Chaiyana, Wantida
Sirithunyalug, Jakkapan
Panyajai, Pawaret
Viriyaadhammaa, Natsima
Nirachonkul, Wariya
Rueankham, Lapamas
Aung, Win Lae
Chueahongthong, Fah
Chiampanichayakul, Sawitree
Anuchapreeda, Songyot
Antileukaemic Cell Proliferation and Cytotoxic Activity of Edible Golden Cordyceps (Cordyceps militaris) Extracts
title Antileukaemic Cell Proliferation and Cytotoxic Activity of Edible Golden Cordyceps (Cordyceps militaris) Extracts
title_full Antileukaemic Cell Proliferation and Cytotoxic Activity of Edible Golden Cordyceps (Cordyceps militaris) Extracts
title_fullStr Antileukaemic Cell Proliferation and Cytotoxic Activity of Edible Golden Cordyceps (Cordyceps militaris) Extracts
title_full_unstemmed Antileukaemic Cell Proliferation and Cytotoxic Activity of Edible Golden Cordyceps (Cordyceps militaris) Extracts
title_short Antileukaemic Cell Proliferation and Cytotoxic Activity of Edible Golden Cordyceps (Cordyceps militaris) Extracts
title_sort antileukaemic cell proliferation and cytotoxic activity of edible golden cordyceps (cordyceps militaris) extracts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054435/
https://www.ncbi.nlm.nih.gov/pubmed/35497915
http://dx.doi.org/10.1155/2022/5347718
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