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Isolation and Characterization of Antibacterial Compounds from Aspergillus fumigatus: An Endophytic Fungus from a Mangrove Plant of the Sundarbans

The Sundarbans, a UNESCO world heritage site, is one of the largest mangrove forests in one stretch. Mangrove plants from this forest are little studied for their endophytic fungi. In this study, we isolated fourteen endophytic fungi from the plants Ceriops decandra and Avicennia officinalis collect...

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Autores principales: Zihad, S. M. Neamul Kabir, Hasan, Md. Towhid, Sultana, Mst Sabiha, Nath, Sushmita, Nahar, Lutfun, Rashid, Mohammad A., Uddin, Shaikh Jamal, Sarker, Satyajit D., Shilpi, Jamil A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054444/
https://www.ncbi.nlm.nih.gov/pubmed/35497914
http://dx.doi.org/10.1155/2022/9600079
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author Zihad, S. M. Neamul Kabir
Hasan, Md. Towhid
Sultana, Mst Sabiha
Nath, Sushmita
Nahar, Lutfun
Rashid, Mohammad A.
Uddin, Shaikh Jamal
Sarker, Satyajit D.
Shilpi, Jamil A.
author_facet Zihad, S. M. Neamul Kabir
Hasan, Md. Towhid
Sultana, Mst Sabiha
Nath, Sushmita
Nahar, Lutfun
Rashid, Mohammad A.
Uddin, Shaikh Jamal
Sarker, Satyajit D.
Shilpi, Jamil A.
author_sort Zihad, S. M. Neamul Kabir
collection PubMed
description The Sundarbans, a UNESCO world heritage site, is one of the largest mangrove forests in one stretch. Mangrove plants from this forest are little studied for their endophytic fungi. In this study, we isolated fourteen endophytic fungi from the plants Ceriops decandra and Avicennia officinalis collected from the Sundarbans. Five of them were identified as Aspergillus sp. and one as Penicillium sp. by macroscopic and microscopic observation. Antibacterial activity of the crude extracts obtained from these endophytes was determined against Staphylococcus aureus, Micrococcus luteus, Escherichia coli, and Pseudomonas aeruginosa using resazurin-based microtiter assay. The isolated endophytes showed varying degrees of antibacterial activity with MICs ranging between 5 and 0.078 mg/mL. Molecular identification of the most active endophyte revealed its identity as Aspergillus fumigatus obtained from the leaves of C. decandra. Acute toxicity study of the ethyl acetate extract of A. fumigatus in mice revealed no mortality even at the highest dose of 2000 mg/kg bodyweight, though some opposing results are found in the subacute toxicity study. The extract was subjected to silica gel and Sephadex column chromatography resulting in the isolation of three pure compounds. LC-MS analysis of these pure compounds revealed their identity as fumigaclavine C, azaspirofuran B, and fraxetin. This is the first report of fraxetin from A. fumigatus. All three identified compounds were previously reported for their antibacterial activity against different strains of both Gram-positive and Gram-negative bacteria. Therefore, the observed antibacterial activity of the ethyl acetate (EtOAc) extract of A. fumigatus could be due to the presence of these compounds. These results support the notion of investigating fungal endophytes from the Sundarbans for new antimicrobial compounds.
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spelling pubmed-90544442022-04-30 Isolation and Characterization of Antibacterial Compounds from Aspergillus fumigatus: An Endophytic Fungus from a Mangrove Plant of the Sundarbans Zihad, S. M. Neamul Kabir Hasan, Md. Towhid Sultana, Mst Sabiha Nath, Sushmita Nahar, Lutfun Rashid, Mohammad A. Uddin, Shaikh Jamal Sarker, Satyajit D. Shilpi, Jamil A. Evid Based Complement Alternat Med Research Article The Sundarbans, a UNESCO world heritage site, is one of the largest mangrove forests in one stretch. Mangrove plants from this forest are little studied for their endophytic fungi. In this study, we isolated fourteen endophytic fungi from the plants Ceriops decandra and Avicennia officinalis collected from the Sundarbans. Five of them were identified as Aspergillus sp. and one as Penicillium sp. by macroscopic and microscopic observation. Antibacterial activity of the crude extracts obtained from these endophytes was determined against Staphylococcus aureus, Micrococcus luteus, Escherichia coli, and Pseudomonas aeruginosa using resazurin-based microtiter assay. The isolated endophytes showed varying degrees of antibacterial activity with MICs ranging between 5 and 0.078 mg/mL. Molecular identification of the most active endophyte revealed its identity as Aspergillus fumigatus obtained from the leaves of C. decandra. Acute toxicity study of the ethyl acetate extract of A. fumigatus in mice revealed no mortality even at the highest dose of 2000 mg/kg bodyweight, though some opposing results are found in the subacute toxicity study. The extract was subjected to silica gel and Sephadex column chromatography resulting in the isolation of three pure compounds. LC-MS analysis of these pure compounds revealed their identity as fumigaclavine C, azaspirofuran B, and fraxetin. This is the first report of fraxetin from A. fumigatus. All three identified compounds were previously reported for their antibacterial activity against different strains of both Gram-positive and Gram-negative bacteria. Therefore, the observed antibacterial activity of the ethyl acetate (EtOAc) extract of A. fumigatus could be due to the presence of these compounds. These results support the notion of investigating fungal endophytes from the Sundarbans for new antimicrobial compounds. Hindawi 2022-04-22 /pmc/articles/PMC9054444/ /pubmed/35497914 http://dx.doi.org/10.1155/2022/9600079 Text en Copyright © 2022 S. M. Neamul Kabir Zihad et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zihad, S. M. Neamul Kabir
Hasan, Md. Towhid
Sultana, Mst Sabiha
Nath, Sushmita
Nahar, Lutfun
Rashid, Mohammad A.
Uddin, Shaikh Jamal
Sarker, Satyajit D.
Shilpi, Jamil A.
Isolation and Characterization of Antibacterial Compounds from Aspergillus fumigatus: An Endophytic Fungus from a Mangrove Plant of the Sundarbans
title Isolation and Characterization of Antibacterial Compounds from Aspergillus fumigatus: An Endophytic Fungus from a Mangrove Plant of the Sundarbans
title_full Isolation and Characterization of Antibacterial Compounds from Aspergillus fumigatus: An Endophytic Fungus from a Mangrove Plant of the Sundarbans
title_fullStr Isolation and Characterization of Antibacterial Compounds from Aspergillus fumigatus: An Endophytic Fungus from a Mangrove Plant of the Sundarbans
title_full_unstemmed Isolation and Characterization of Antibacterial Compounds from Aspergillus fumigatus: An Endophytic Fungus from a Mangrove Plant of the Sundarbans
title_short Isolation and Characterization of Antibacterial Compounds from Aspergillus fumigatus: An Endophytic Fungus from a Mangrove Plant of the Sundarbans
title_sort isolation and characterization of antibacterial compounds from aspergillus fumigatus: an endophytic fungus from a mangrove plant of the sundarbans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054444/
https://www.ncbi.nlm.nih.gov/pubmed/35497914
http://dx.doi.org/10.1155/2022/9600079
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