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Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells

Mesangial proliferative glomerulonephritis (MesPGN) is a common renal disease that lacks effective drug intervention. Aconiti Lateralis Radix (Fuzi), a natural Chinese medical herb, is found with significant therapeutic effects on various diseases in the clinic. However, its effects on MesPGN have n...

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Autores principales: Li, Xingyao, An, Peng, Zhao, Yanhong, Cai, Zimo, Ye, Bingyu, Wang, Yafeng, Wang, Wenfang, Gao, Qi, Li, Liuyun, Zhang, Tao, Wu, Xili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054446/
https://www.ncbi.nlm.nih.gov/pubmed/35497917
http://dx.doi.org/10.1155/2022/3664696
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author Li, Xingyao
An, Peng
Zhao, Yanhong
Cai, Zimo
Ye, Bingyu
Wang, Yafeng
Wang, Wenfang
Gao, Qi
Li, Liuyun
Zhang, Tao
Wu, Xili
author_facet Li, Xingyao
An, Peng
Zhao, Yanhong
Cai, Zimo
Ye, Bingyu
Wang, Yafeng
Wang, Wenfang
Gao, Qi
Li, Liuyun
Zhang, Tao
Wu, Xili
author_sort Li, Xingyao
collection PubMed
description Mesangial proliferative glomerulonephritis (MesPGN) is a common renal disease that lacks effective drug intervention. Aconiti Lateralis Radix (Fuzi), a natural Chinese medical herb, is found with significant therapeutic effects on various diseases in the clinic. However, its effects on MesPGN have not been reported. This study is aimed to discuss the therapeutic effects of the aqueous extract of Aconiti Lateralis Radix (ALR) and the polysaccharides of Aconiti Lateralis Radix (PALR) on MesPGN as well as the underlying mechanism. In this study, we, firstly, studied the anti-MesPGN mechanism of ALR and PALR. ALR and PALR inhibit the proliferation of the mesangial cells through the PI3K/AKT/mTOR pathway, induce the G0/G1 phase of block and apoptosis, inhibit the activity of Cyclin E and CDK2, increase the expression of Bax, cleaved caspase-8/caspase-8, and cleaved caspase-3/caspase-3 proteins, and effectively inhibit the growth of the mesangial cells. Overall, our data suggest that ALR and PALR may be potential candidates for MesPGN and that PALR is more effective than ALR.
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spelling pubmed-90544462022-04-30 Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells Li, Xingyao An, Peng Zhao, Yanhong Cai, Zimo Ye, Bingyu Wang, Yafeng Wang, Wenfang Gao, Qi Li, Liuyun Zhang, Tao Wu, Xili Evid Based Complement Alternat Med Research Article Mesangial proliferative glomerulonephritis (MesPGN) is a common renal disease that lacks effective drug intervention. Aconiti Lateralis Radix (Fuzi), a natural Chinese medical herb, is found with significant therapeutic effects on various diseases in the clinic. However, its effects on MesPGN have not been reported. This study is aimed to discuss the therapeutic effects of the aqueous extract of Aconiti Lateralis Radix (ALR) and the polysaccharides of Aconiti Lateralis Radix (PALR) on MesPGN as well as the underlying mechanism. In this study, we, firstly, studied the anti-MesPGN mechanism of ALR and PALR. ALR and PALR inhibit the proliferation of the mesangial cells through the PI3K/AKT/mTOR pathway, induce the G0/G1 phase of block and apoptosis, inhibit the activity of Cyclin E and CDK2, increase the expression of Bax, cleaved caspase-8/caspase-8, and cleaved caspase-3/caspase-3 proteins, and effectively inhibit the growth of the mesangial cells. Overall, our data suggest that ALR and PALR may be potential candidates for MesPGN and that PALR is more effective than ALR. Hindawi 2022-04-22 /pmc/articles/PMC9054446/ /pubmed/35497917 http://dx.doi.org/10.1155/2022/3664696 Text en Copyright © 2022 Xingyao Li et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Xingyao
An, Peng
Zhao, Yanhong
Cai, Zimo
Ye, Bingyu
Wang, Yafeng
Wang, Wenfang
Gao, Qi
Li, Liuyun
Zhang, Tao
Wu, Xili
Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells
title Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells
title_full Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells
title_fullStr Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells
title_full_unstemmed Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells
title_short Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells
title_sort aqueous extract and polysaccharide of aconiti lateralis radix induce apoptosis and g0/g1 phase cell cycle arrest by pi3k/akt/mtor signaling pathway in mesangial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054446/
https://www.ncbi.nlm.nih.gov/pubmed/35497917
http://dx.doi.org/10.1155/2022/3664696
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