Cargando…
Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells
Mesangial proliferative glomerulonephritis (MesPGN) is a common renal disease that lacks effective drug intervention. Aconiti Lateralis Radix (Fuzi), a natural Chinese medical herb, is found with significant therapeutic effects on various diseases in the clinic. However, its effects on MesPGN have n...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054446/ https://www.ncbi.nlm.nih.gov/pubmed/35497917 http://dx.doi.org/10.1155/2022/3664696 |
_version_ | 1784697188415176704 |
---|---|
author | Li, Xingyao An, Peng Zhao, Yanhong Cai, Zimo Ye, Bingyu Wang, Yafeng Wang, Wenfang Gao, Qi Li, Liuyun Zhang, Tao Wu, Xili |
author_facet | Li, Xingyao An, Peng Zhao, Yanhong Cai, Zimo Ye, Bingyu Wang, Yafeng Wang, Wenfang Gao, Qi Li, Liuyun Zhang, Tao Wu, Xili |
author_sort | Li, Xingyao |
collection | PubMed |
description | Mesangial proliferative glomerulonephritis (MesPGN) is a common renal disease that lacks effective drug intervention. Aconiti Lateralis Radix (Fuzi), a natural Chinese medical herb, is found with significant therapeutic effects on various diseases in the clinic. However, its effects on MesPGN have not been reported. This study is aimed to discuss the therapeutic effects of the aqueous extract of Aconiti Lateralis Radix (ALR) and the polysaccharides of Aconiti Lateralis Radix (PALR) on MesPGN as well as the underlying mechanism. In this study, we, firstly, studied the anti-MesPGN mechanism of ALR and PALR. ALR and PALR inhibit the proliferation of the mesangial cells through the PI3K/AKT/mTOR pathway, induce the G0/G1 phase of block and apoptosis, inhibit the activity of Cyclin E and CDK2, increase the expression of Bax, cleaved caspase-8/caspase-8, and cleaved caspase-3/caspase-3 proteins, and effectively inhibit the growth of the mesangial cells. Overall, our data suggest that ALR and PALR may be potential candidates for MesPGN and that PALR is more effective than ALR. |
format | Online Article Text |
id | pubmed-9054446 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-90544462022-04-30 Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells Li, Xingyao An, Peng Zhao, Yanhong Cai, Zimo Ye, Bingyu Wang, Yafeng Wang, Wenfang Gao, Qi Li, Liuyun Zhang, Tao Wu, Xili Evid Based Complement Alternat Med Research Article Mesangial proliferative glomerulonephritis (MesPGN) is a common renal disease that lacks effective drug intervention. Aconiti Lateralis Radix (Fuzi), a natural Chinese medical herb, is found with significant therapeutic effects on various diseases in the clinic. However, its effects on MesPGN have not been reported. This study is aimed to discuss the therapeutic effects of the aqueous extract of Aconiti Lateralis Radix (ALR) and the polysaccharides of Aconiti Lateralis Radix (PALR) on MesPGN as well as the underlying mechanism. In this study, we, firstly, studied the anti-MesPGN mechanism of ALR and PALR. ALR and PALR inhibit the proliferation of the mesangial cells through the PI3K/AKT/mTOR pathway, induce the G0/G1 phase of block and apoptosis, inhibit the activity of Cyclin E and CDK2, increase the expression of Bax, cleaved caspase-8/caspase-8, and cleaved caspase-3/caspase-3 proteins, and effectively inhibit the growth of the mesangial cells. Overall, our data suggest that ALR and PALR may be potential candidates for MesPGN and that PALR is more effective than ALR. Hindawi 2022-04-22 /pmc/articles/PMC9054446/ /pubmed/35497917 http://dx.doi.org/10.1155/2022/3664696 Text en Copyright © 2022 Xingyao Li et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Li, Xingyao An, Peng Zhao, Yanhong Cai, Zimo Ye, Bingyu Wang, Yafeng Wang, Wenfang Gao, Qi Li, Liuyun Zhang, Tao Wu, Xili Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells |
title | Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells |
title_full | Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells |
title_fullStr | Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells |
title_full_unstemmed | Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells |
title_short | Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells |
title_sort | aqueous extract and polysaccharide of aconiti lateralis radix induce apoptosis and g0/g1 phase cell cycle arrest by pi3k/akt/mtor signaling pathway in mesangial cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054446/ https://www.ncbi.nlm.nih.gov/pubmed/35497917 http://dx.doi.org/10.1155/2022/3664696 |
work_keys_str_mv | AT lixingyao aqueousextractandpolysaccharideofaconitilateralisradixinduceapoptosisandg0g1phasecellcyclearrestbypi3kaktmtorsignalingpathwayinmesangialcells AT anpeng aqueousextractandpolysaccharideofaconitilateralisradixinduceapoptosisandg0g1phasecellcyclearrestbypi3kaktmtorsignalingpathwayinmesangialcells AT zhaoyanhong aqueousextractandpolysaccharideofaconitilateralisradixinduceapoptosisandg0g1phasecellcyclearrestbypi3kaktmtorsignalingpathwayinmesangialcells AT caizimo aqueousextractandpolysaccharideofaconitilateralisradixinduceapoptosisandg0g1phasecellcyclearrestbypi3kaktmtorsignalingpathwayinmesangialcells AT yebingyu aqueousextractandpolysaccharideofaconitilateralisradixinduceapoptosisandg0g1phasecellcyclearrestbypi3kaktmtorsignalingpathwayinmesangialcells AT wangyafeng aqueousextractandpolysaccharideofaconitilateralisradixinduceapoptosisandg0g1phasecellcyclearrestbypi3kaktmtorsignalingpathwayinmesangialcells AT wangwenfang aqueousextractandpolysaccharideofaconitilateralisradixinduceapoptosisandg0g1phasecellcyclearrestbypi3kaktmtorsignalingpathwayinmesangialcells AT gaoqi aqueousextractandpolysaccharideofaconitilateralisradixinduceapoptosisandg0g1phasecellcyclearrestbypi3kaktmtorsignalingpathwayinmesangialcells AT liliuyun aqueousextractandpolysaccharideofaconitilateralisradixinduceapoptosisandg0g1phasecellcyclearrestbypi3kaktmtorsignalingpathwayinmesangialcells AT zhangtao aqueousextractandpolysaccharideofaconitilateralisradixinduceapoptosisandg0g1phasecellcyclearrestbypi3kaktmtorsignalingpathwayinmesangialcells AT wuxili aqueousextractandpolysaccharideofaconitilateralisradixinduceapoptosisandg0g1phasecellcyclearrestbypi3kaktmtorsignalingpathwayinmesangialcells |