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In vitro evaluation of novel low-pressure spark plasma sintered HA–BG composite scaffolds for bone tissue engineering

The low-pressure spark plasma sintering (SPS) technique is adopted to fabricate hydroxyapatite–bioglass (HA–BG) scaffolds while maintaining the physical properties of both components, including their bulk and relative density and hardness. However, prior to their orthopaedic and dental applications,...

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Autores principales: Rizwan, Muhammad, Genasan, Krishnamurithy, Murali, Malliga Raman, Balaji Raghavendran, Hanumantha Rao, Alias, Rodianah, Cheok, Yi Ying, Wong, Won Fen, Mansor, Azura, Hamdi, M., Basirun, Wan Jeffrey, Kamarul, Tunku
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054734/
https://www.ncbi.nlm.nih.gov/pubmed/35517330
http://dx.doi.org/10.1039/d0ra04227g
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author Rizwan, Muhammad
Genasan, Krishnamurithy
Murali, Malliga Raman
Balaji Raghavendran, Hanumantha Rao
Alias, Rodianah
Cheok, Yi Ying
Wong, Won Fen
Mansor, Azura
Hamdi, M.
Basirun, Wan Jeffrey
Kamarul, Tunku
author_facet Rizwan, Muhammad
Genasan, Krishnamurithy
Murali, Malliga Raman
Balaji Raghavendran, Hanumantha Rao
Alias, Rodianah
Cheok, Yi Ying
Wong, Won Fen
Mansor, Azura
Hamdi, M.
Basirun, Wan Jeffrey
Kamarul, Tunku
author_sort Rizwan, Muhammad
collection PubMed
description The low-pressure spark plasma sintering (SPS) technique is adopted to fabricate hydroxyapatite–bioglass (HA–BG) scaffolds while maintaining the physical properties of both components, including their bulk and relative density and hardness. However, prior to their orthopaedic and dental applications, these scaffolds must be validated via pre-clinical assessments. In the present study, scaffolds with different ratios of HA : BG, namely, 100 : 0 (HB 0 S), 90 : 10 (HB 10 S), 80 : 20 (HB 20 S) and 70 : 30 (HB 30 S) were fabricated. These scaffolds were characterized by investigating their physicochemical properties (X-ray diffraction (XRD) and surface wettability), bioactivity in a simulated body fluid (SBF) (field emission scanning electron microscopy (FESEM), Fourier-transform infrared spectroscopy (FTIR) and calcium dissolution), antimicrobial properties, biocompatibility and osteoinduction of human bone marrow-derived mesenchymal stromal cells (hBMSCs) and human monocyte immune cell response. The XRD and surface wettability results confirmed no formation of undesirable phases and the enhanced surface hydrophilicity of the scaffolds, respectively. The bioactivity in SBF indicated the formation of bone-like apatite on the surface of the scaffolds, corresponding to an increase in BG%, which was confirmed through FTIR spectra and the increasing trend of calcium release in SBF. The scaffolds showed inhibition properties against Staphylococcus aureus and Staphylococcus epidermidis. The scanning electron microscopy (SEM) micrographs and Alamar Blue proliferation assay indicated the good attachment and significant proliferation, respectively, of hBMSCs on the scaffolds. Alizarin Red S staining confirmed that the scaffolds supported the mineralisation of hBMSCs. The osteogenic protein secretion (bone morphogenetic protein-2 (BMP2), type-I collagen (COL1) and osterix (OSX)) was significant on the HB 30 S-seeded hBMSCs when compared with that of HB 0 S. The monocyte migration was significantly halted in response to HA–BG-conditioned media when compared with the positive control (monocyte chemoattractant protein-1: MCP-1). In conclusion, the HB 30 S composite scaffold has a greater potential to substitute bone grafts in orthopaedic and dental applications.
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spelling pubmed-90547342022-05-04 In vitro evaluation of novel low-pressure spark plasma sintered HA–BG composite scaffolds for bone tissue engineering Rizwan, Muhammad Genasan, Krishnamurithy Murali, Malliga Raman Balaji Raghavendran, Hanumantha Rao Alias, Rodianah Cheok, Yi Ying Wong, Won Fen Mansor, Azura Hamdi, M. Basirun, Wan Jeffrey Kamarul, Tunku RSC Adv Chemistry The low-pressure spark plasma sintering (SPS) technique is adopted to fabricate hydroxyapatite–bioglass (HA–BG) scaffolds while maintaining the physical properties of both components, including their bulk and relative density and hardness. However, prior to their orthopaedic and dental applications, these scaffolds must be validated via pre-clinical assessments. In the present study, scaffolds with different ratios of HA : BG, namely, 100 : 0 (HB 0 S), 90 : 10 (HB 10 S), 80 : 20 (HB 20 S) and 70 : 30 (HB 30 S) were fabricated. These scaffolds were characterized by investigating their physicochemical properties (X-ray diffraction (XRD) and surface wettability), bioactivity in a simulated body fluid (SBF) (field emission scanning electron microscopy (FESEM), Fourier-transform infrared spectroscopy (FTIR) and calcium dissolution), antimicrobial properties, biocompatibility and osteoinduction of human bone marrow-derived mesenchymal stromal cells (hBMSCs) and human monocyte immune cell response. The XRD and surface wettability results confirmed no formation of undesirable phases and the enhanced surface hydrophilicity of the scaffolds, respectively. The bioactivity in SBF indicated the formation of bone-like apatite on the surface of the scaffolds, corresponding to an increase in BG%, which was confirmed through FTIR spectra and the increasing trend of calcium release in SBF. The scaffolds showed inhibition properties against Staphylococcus aureus and Staphylococcus epidermidis. The scanning electron microscopy (SEM) micrographs and Alamar Blue proliferation assay indicated the good attachment and significant proliferation, respectively, of hBMSCs on the scaffolds. Alizarin Red S staining confirmed that the scaffolds supported the mineralisation of hBMSCs. The osteogenic protein secretion (bone morphogenetic protein-2 (BMP2), type-I collagen (COL1) and osterix (OSX)) was significant on the HB 30 S-seeded hBMSCs when compared with that of HB 0 S. The monocyte migration was significantly halted in response to HA–BG-conditioned media when compared with the positive control (monocyte chemoattractant protein-1: MCP-1). In conclusion, the HB 30 S composite scaffold has a greater potential to substitute bone grafts in orthopaedic and dental applications. The Royal Society of Chemistry 2020-06-23 /pmc/articles/PMC9054734/ /pubmed/35517330 http://dx.doi.org/10.1039/d0ra04227g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Rizwan, Muhammad
Genasan, Krishnamurithy
Murali, Malliga Raman
Balaji Raghavendran, Hanumantha Rao
Alias, Rodianah
Cheok, Yi Ying
Wong, Won Fen
Mansor, Azura
Hamdi, M.
Basirun, Wan Jeffrey
Kamarul, Tunku
In vitro evaluation of novel low-pressure spark plasma sintered HA–BG composite scaffolds for bone tissue engineering
title In vitro evaluation of novel low-pressure spark plasma sintered HA–BG composite scaffolds for bone tissue engineering
title_full In vitro evaluation of novel low-pressure spark plasma sintered HA–BG composite scaffolds for bone tissue engineering
title_fullStr In vitro evaluation of novel low-pressure spark plasma sintered HA–BG composite scaffolds for bone tissue engineering
title_full_unstemmed In vitro evaluation of novel low-pressure spark plasma sintered HA–BG composite scaffolds for bone tissue engineering
title_short In vitro evaluation of novel low-pressure spark plasma sintered HA–BG composite scaffolds for bone tissue engineering
title_sort in vitro evaluation of novel low-pressure spark plasma sintered ha–bg composite scaffolds for bone tissue engineering
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054734/
https://www.ncbi.nlm.nih.gov/pubmed/35517330
http://dx.doi.org/10.1039/d0ra04227g
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