Enzymatic synthesis of 2-hydroxy-4H-quinolizin-4-one scaffolds by integrating coenzyme a ligases and a type III PKS from Huperzia serrata

2-Hydroxy-4H-quinolizin-4-one scaffolds were enzymatically synthesized by integrating three enzymes including phenylacetate-CoA ligase (PcPCL) from an endophytic fungus Penicillium chrysogenum MT-12, malonyl-CoA synthase (AtMatB) from Arabidopsis thaliana, and a type III polyketide synthase (HsPKS3)...

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Autores principales: Wang, Juan, Ding, Ning, Wu, Yun, Shi, Xiaoping, Qi, Bowen, Liu, Xiao, Wang, Xiaohui, Li, Jun, Tu, Pengfei, Shi, Shepo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054772/
https://www.ncbi.nlm.nih.gov/pubmed/35517366
http://dx.doi.org/10.1039/d0ra04133e
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author Wang, Juan
Ding, Ning
Wu, Yun
Shi, Xiaoping
Qi, Bowen
Liu, Xiao
Wang, Xiaohui
Li, Jun
Tu, Pengfei
Shi, Shepo
author_facet Wang, Juan
Ding, Ning
Wu, Yun
Shi, Xiaoping
Qi, Bowen
Liu, Xiao
Wang, Xiaohui
Li, Jun
Tu, Pengfei
Shi, Shepo
author_sort Wang, Juan
collection PubMed
description 2-Hydroxy-4H-quinolizin-4-one scaffolds were enzymatically synthesized by integrating three enzymes including phenylacetate-CoA ligase (PcPCL) from an endophytic fungus Penicillium chrysogenum MT-12, malonyl-CoA synthase (AtMatB) from Arabidopsis thaliana, and a type III polyketide synthase (HsPKS3) from Chinese club moss Huperzia serrata. The findings paved the way to produce these kinds of structurally interesting alkaloids by engineered microorganisms.
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spelling pubmed-90547722022-05-04 Enzymatic synthesis of 2-hydroxy-4H-quinolizin-4-one scaffolds by integrating coenzyme a ligases and a type III PKS from Huperzia serrata Wang, Juan Ding, Ning Wu, Yun Shi, Xiaoping Qi, Bowen Liu, Xiao Wang, Xiaohui Li, Jun Tu, Pengfei Shi, Shepo RSC Adv Chemistry 2-Hydroxy-4H-quinolizin-4-one scaffolds were enzymatically synthesized by integrating three enzymes including phenylacetate-CoA ligase (PcPCL) from an endophytic fungus Penicillium chrysogenum MT-12, malonyl-CoA synthase (AtMatB) from Arabidopsis thaliana, and a type III polyketide synthase (HsPKS3) from Chinese club moss Huperzia serrata. The findings paved the way to produce these kinds of structurally interesting alkaloids by engineered microorganisms. The Royal Society of Chemistry 2020-06-22 /pmc/articles/PMC9054772/ /pubmed/35517366 http://dx.doi.org/10.1039/d0ra04133e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Wang, Juan
Ding, Ning
Wu, Yun
Shi, Xiaoping
Qi, Bowen
Liu, Xiao
Wang, Xiaohui
Li, Jun
Tu, Pengfei
Shi, Shepo
Enzymatic synthesis of 2-hydroxy-4H-quinolizin-4-one scaffolds by integrating coenzyme a ligases and a type III PKS from Huperzia serrata
title Enzymatic synthesis of 2-hydroxy-4H-quinolizin-4-one scaffolds by integrating coenzyme a ligases and a type III PKS from Huperzia serrata
title_full Enzymatic synthesis of 2-hydroxy-4H-quinolizin-4-one scaffolds by integrating coenzyme a ligases and a type III PKS from Huperzia serrata
title_fullStr Enzymatic synthesis of 2-hydroxy-4H-quinolizin-4-one scaffolds by integrating coenzyme a ligases and a type III PKS from Huperzia serrata
title_full_unstemmed Enzymatic synthesis of 2-hydroxy-4H-quinolizin-4-one scaffolds by integrating coenzyme a ligases and a type III PKS from Huperzia serrata
title_short Enzymatic synthesis of 2-hydroxy-4H-quinolizin-4-one scaffolds by integrating coenzyme a ligases and a type III PKS from Huperzia serrata
title_sort enzymatic synthesis of 2-hydroxy-4h-quinolizin-4-one scaffolds by integrating coenzyme a ligases and a type iii pks from huperzia serrata
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9054772/
https://www.ncbi.nlm.nih.gov/pubmed/35517366
http://dx.doi.org/10.1039/d0ra04133e
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