Cargando…
Intervention effects of lotus leaf flavonoids on gastric mucosal lesions in mice infected with Helicobacter pylori
Helicobacter pylori (H. pylori) is one of the main factors that cause gastric lesions. The lotus leaf is an edible plant used in traditional Eastern medicine. This study evaluates the intervention effects of lotus leaf flavonoids (LLF) on gastric mucosal lesions in mice infected with H. pylori and e...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055112/ https://www.ncbi.nlm.nih.gov/pubmed/35517367 http://dx.doi.org/10.1039/d0ra03311a |
_version_ | 1784697332011368448 |
---|---|
author | Yi, Ruokun Wang, Feng-Bo Tan, Fang Long, Xingyao Pan, Yanni Zhao, Xin |
author_facet | Yi, Ruokun Wang, Feng-Bo Tan, Fang Long, Xingyao Pan, Yanni Zhao, Xin |
author_sort | Yi, Ruokun |
collection | PubMed |
description | Helicobacter pylori (H. pylori) is one of the main factors that cause gastric lesions. The lotus leaf is an edible plant used in traditional Eastern medicine. This study evaluates the intervention effects of lotus leaf flavonoids (LLF) on gastric mucosal lesions in mice infected with H. pylori and explores their mechanism of action. High-performance liquid chromatography analysis reveals that LLF contain kaempferitrin (kaempferol-3,7-dirhamnoside), hypericin, astragalin (kaempferol-3-glucoside), phlorizin, and quercetin. LLF can reduce the number of gastric mucosal lesions and tissue lesions in mice with H. pylori-induced gastric lesions. LLF can increase the levels of somatostatin and vasoactive intestinal peptide in the serum of mice with gastric lesions and decrease the levels of substance P and endothelin-1 to inhibit gastric lesions. LLF can also reduce the levels of interleukin (IL)-6, IL-12, tumor necrosis factor (TNF)-α, and interferon-gamma cytokines in the serum of mice with gastric lesions. Using a quantitative polymerase chain reaction assay it can be seen that LLF can downregulate mRNA expressions of TNF-α, IL-1β, myeloperoxidase, keratin (KRT) 16, KRT6b, and transglutaminase 3 epidermal in the gastric tissues of mice with gastric lesions. Western blot analysis indicates that LLF can downregulate the protein expressions of caspase-1, Nod-like receptor protein 3, IL-1β, TNF-α, and Toll-like receptor 4 in the gastric tissues of mice with gastric lesions. LLF have beneficial effects on gastric lesions induced by H. pylori. Meanwhile LLF is more active in competition with ranitidine. LLF represent an active substance that can inhibit H. pylori-induced gastric lesions. The flavones of LLF may enhance the inhibition of gastric mucosal lesions by promoting the interaction between the compounds. |
format | Online Article Text |
id | pubmed-9055112 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90551122022-05-04 Intervention effects of lotus leaf flavonoids on gastric mucosal lesions in mice infected with Helicobacter pylori Yi, Ruokun Wang, Feng-Bo Tan, Fang Long, Xingyao Pan, Yanni Zhao, Xin RSC Adv Chemistry Helicobacter pylori (H. pylori) is one of the main factors that cause gastric lesions. The lotus leaf is an edible plant used in traditional Eastern medicine. This study evaluates the intervention effects of lotus leaf flavonoids (LLF) on gastric mucosal lesions in mice infected with H. pylori and explores their mechanism of action. High-performance liquid chromatography analysis reveals that LLF contain kaempferitrin (kaempferol-3,7-dirhamnoside), hypericin, astragalin (kaempferol-3-glucoside), phlorizin, and quercetin. LLF can reduce the number of gastric mucosal lesions and tissue lesions in mice with H. pylori-induced gastric lesions. LLF can increase the levels of somatostatin and vasoactive intestinal peptide in the serum of mice with gastric lesions and decrease the levels of substance P and endothelin-1 to inhibit gastric lesions. LLF can also reduce the levels of interleukin (IL)-6, IL-12, tumor necrosis factor (TNF)-α, and interferon-gamma cytokines in the serum of mice with gastric lesions. Using a quantitative polymerase chain reaction assay it can be seen that LLF can downregulate mRNA expressions of TNF-α, IL-1β, myeloperoxidase, keratin (KRT) 16, KRT6b, and transglutaminase 3 epidermal in the gastric tissues of mice with gastric lesions. Western blot analysis indicates that LLF can downregulate the protein expressions of caspase-1, Nod-like receptor protein 3, IL-1β, TNF-α, and Toll-like receptor 4 in the gastric tissues of mice with gastric lesions. LLF have beneficial effects on gastric lesions induced by H. pylori. Meanwhile LLF is more active in competition with ranitidine. LLF represent an active substance that can inhibit H. pylori-induced gastric lesions. The flavones of LLF may enhance the inhibition of gastric mucosal lesions by promoting the interaction between the compounds. The Royal Society of Chemistry 2020-06-19 /pmc/articles/PMC9055112/ /pubmed/35517367 http://dx.doi.org/10.1039/d0ra03311a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Yi, Ruokun Wang, Feng-Bo Tan, Fang Long, Xingyao Pan, Yanni Zhao, Xin Intervention effects of lotus leaf flavonoids on gastric mucosal lesions in mice infected with Helicobacter pylori |
title | Intervention effects of lotus leaf flavonoids on gastric mucosal lesions in mice infected with Helicobacter pylori |
title_full | Intervention effects of lotus leaf flavonoids on gastric mucosal lesions in mice infected with Helicobacter pylori |
title_fullStr | Intervention effects of lotus leaf flavonoids on gastric mucosal lesions in mice infected with Helicobacter pylori |
title_full_unstemmed | Intervention effects of lotus leaf flavonoids on gastric mucosal lesions in mice infected with Helicobacter pylori |
title_short | Intervention effects of lotus leaf flavonoids on gastric mucosal lesions in mice infected with Helicobacter pylori |
title_sort | intervention effects of lotus leaf flavonoids on gastric mucosal lesions in mice infected with helicobacter pylori |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055112/ https://www.ncbi.nlm.nih.gov/pubmed/35517367 http://dx.doi.org/10.1039/d0ra03311a |
work_keys_str_mv | AT yiruokun interventioneffectsoflotusleafflavonoidsongastricmucosallesionsinmiceinfectedwithhelicobacterpylori AT wangfengbo interventioneffectsoflotusleafflavonoidsongastricmucosallesionsinmiceinfectedwithhelicobacterpylori AT tanfang interventioneffectsoflotusleafflavonoidsongastricmucosallesionsinmiceinfectedwithhelicobacterpylori AT longxingyao interventioneffectsoflotusleafflavonoidsongastricmucosallesionsinmiceinfectedwithhelicobacterpylori AT panyanni interventioneffectsoflotusleafflavonoidsongastricmucosallesionsinmiceinfectedwithhelicobacterpylori AT zhaoxin interventioneffectsoflotusleafflavonoidsongastricmucosallesionsinmiceinfectedwithhelicobacterpylori |