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An effective biocompatible fluorescent probe for bisulfite detection in aqueous solution, living cells, and mice

Sulfur dioxide, an air pollutant, is easily hydrated to sulfites and bisulfites and extremely harmful to human health. On the other hand, endogenous sulfur dioxide is the fourth gasotransmitter. In view of the above, it is worth developing an effective method for the detection of these compounds. In...

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Autores principales: Zhou, Ruqiao, Cui, Guiling, Hu, Yuefu, Qi, Qingrong, Huang, Wencai, Yang, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055347/
https://www.ncbi.nlm.nih.gov/pubmed/35517487
http://dx.doi.org/10.1039/d0ra03329d
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author Zhou, Ruqiao
Cui, Guiling
Hu, Yuefu
Qi, Qingrong
Huang, Wencai
Yang, Li
author_facet Zhou, Ruqiao
Cui, Guiling
Hu, Yuefu
Qi, Qingrong
Huang, Wencai
Yang, Li
author_sort Zhou, Ruqiao
collection PubMed
description Sulfur dioxide, an air pollutant, is easily hydrated to sulfites and bisulfites and extremely harmful to human health. On the other hand, endogenous sulfur dioxide is the fourth gasotransmitter. In view of the above, it is worth developing an effective method for the detection of these compounds. In this paper, a novel colorimetric fluorescent probe (Hcy-Mo), based on hemi-cyanine, for bisulfites is reported. Hcy-Mo shows excellent selectivity for bisulfites over various other species including cysteine, glutathione, CN(−), and HS(−), and undergoes 1,4-addition reactions at the C-4 atom of the ethylene group. The reaction can be completed in 30 s in a PBS buffer solution and displays high sensitivity (limit of detection is 80 nM) for bisulfites. Test paper experiments show that the probe can be used for bisulfite detection in aqueous solutions. In addition, Hcy-Mo exhibits excellent cell permeability and low cytotoxicity for the successful detection of bisulfites in living MDA-MB-231 cells and in living mice, implying that this probe would be of great benefit to biological researchers for investigating the detailed biological and pharmacological functions of bisulfites in biological systems.
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spelling pubmed-90553472022-05-04 An effective biocompatible fluorescent probe for bisulfite detection in aqueous solution, living cells, and mice Zhou, Ruqiao Cui, Guiling Hu, Yuefu Qi, Qingrong Huang, Wencai Yang, Li RSC Adv Chemistry Sulfur dioxide, an air pollutant, is easily hydrated to sulfites and bisulfites and extremely harmful to human health. On the other hand, endogenous sulfur dioxide is the fourth gasotransmitter. In view of the above, it is worth developing an effective method for the detection of these compounds. In this paper, a novel colorimetric fluorescent probe (Hcy-Mo), based on hemi-cyanine, for bisulfites is reported. Hcy-Mo shows excellent selectivity for bisulfites over various other species including cysteine, glutathione, CN(−), and HS(−), and undergoes 1,4-addition reactions at the C-4 atom of the ethylene group. The reaction can be completed in 30 s in a PBS buffer solution and displays high sensitivity (limit of detection is 80 nM) for bisulfites. Test paper experiments show that the probe can be used for bisulfite detection in aqueous solutions. In addition, Hcy-Mo exhibits excellent cell permeability and low cytotoxicity for the successful detection of bisulfites in living MDA-MB-231 cells and in living mice, implying that this probe would be of great benefit to biological researchers for investigating the detailed biological and pharmacological functions of bisulfites in biological systems. The Royal Society of Chemistry 2020-07-03 /pmc/articles/PMC9055347/ /pubmed/35517487 http://dx.doi.org/10.1039/d0ra03329d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Zhou, Ruqiao
Cui, Guiling
Hu, Yuefu
Qi, Qingrong
Huang, Wencai
Yang, Li
An effective biocompatible fluorescent probe for bisulfite detection in aqueous solution, living cells, and mice
title An effective biocompatible fluorescent probe for bisulfite detection in aqueous solution, living cells, and mice
title_full An effective biocompatible fluorescent probe for bisulfite detection in aqueous solution, living cells, and mice
title_fullStr An effective biocompatible fluorescent probe for bisulfite detection in aqueous solution, living cells, and mice
title_full_unstemmed An effective biocompatible fluorescent probe for bisulfite detection in aqueous solution, living cells, and mice
title_short An effective biocompatible fluorescent probe for bisulfite detection in aqueous solution, living cells, and mice
title_sort effective biocompatible fluorescent probe for bisulfite detection in aqueous solution, living cells, and mice
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055347/
https://www.ncbi.nlm.nih.gov/pubmed/35517487
http://dx.doi.org/10.1039/d0ra03329d
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