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Inventing a facile method to construct Bombyx mori (B. mori) silk fibroin nanocapsules for drug delivery

Bombyx mori (B. mori) silk fibroin (SF) microcapsules have acted as a great candidate in delivering drugs. However, it is difficult to fabricate SF nanocapsules using the present layer-by-layer (LBL) technique. In addition, the current SF microcapsules have limits in loading negatively charged drugs...

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Detalles Bibliográficos
Autores principales: Zheng, Heming, Duan, Bo, Xie, Zheyu, Wang, Jie, Yang, Mingying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055660/
https://www.ncbi.nlm.nih.gov/pubmed/35519128
http://dx.doi.org/10.1039/d0ra04024j
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author Zheng, Heming
Duan, Bo
Xie, Zheyu
Wang, Jie
Yang, Mingying
author_facet Zheng, Heming
Duan, Bo
Xie, Zheyu
Wang, Jie
Yang, Mingying
author_sort Zheng, Heming
collection PubMed
description Bombyx mori (B. mori) silk fibroin (SF) microcapsules have acted as a great candidate in delivering drugs. However, it is difficult to fabricate SF nanocapsules using the present layer-by-layer (LBL) technique. In addition, the current SF microcapsules have limits in loading negatively charged drugs. Here, we invent a novel LBL method by introducing silane (APTES) as a structure indicator to produce SF nanocapsules that can load drugs with negative or positive charge. LBL assembly was completed by alternately coating SF and APTES on the template of polystyrene (PS) nanospheres by electrostatic attraction. SF nanocapsules were obtained after removal of the PS templates. Zeta potential analysis proved LBL assembly was indeed driven by the interaction between negative charge of SF and positive charge of APTES. Fluorescence images and electric microscope images indicated that SF nanocapsules had a hollow and stable structure with diameter at nearly 250 nm. The highest encapsulation rate of DOX or Ce6 were up to 80% and 90%, respectively, indicating SF nanocapsules have a high loading capability for both cationic and anionic drugs. In vitro cell experiments proved the biocompatibility of SF nanocapsules and their burst drug release in response to acidic environment. Furthermore, chemotherapy and photodynamic therapy proved SF nanocapsules loaded with DOX or Ce6 had significant inhibition on tumor cells. Our results suggested that this LBL technique is a facile method for polymers with negative charge to fabricate nanocapsules for antitumor drug carrier.
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spelling pubmed-90556602022-05-04 Inventing a facile method to construct Bombyx mori (B. mori) silk fibroin nanocapsules for drug delivery Zheng, Heming Duan, Bo Xie, Zheyu Wang, Jie Yang, Mingying RSC Adv Chemistry Bombyx mori (B. mori) silk fibroin (SF) microcapsules have acted as a great candidate in delivering drugs. However, it is difficult to fabricate SF nanocapsules using the present layer-by-layer (LBL) technique. In addition, the current SF microcapsules have limits in loading negatively charged drugs. Here, we invent a novel LBL method by introducing silane (APTES) as a structure indicator to produce SF nanocapsules that can load drugs with negative or positive charge. LBL assembly was completed by alternately coating SF and APTES on the template of polystyrene (PS) nanospheres by electrostatic attraction. SF nanocapsules were obtained after removal of the PS templates. Zeta potential analysis proved LBL assembly was indeed driven by the interaction between negative charge of SF and positive charge of APTES. Fluorescence images and electric microscope images indicated that SF nanocapsules had a hollow and stable structure with diameter at nearly 250 nm. The highest encapsulation rate of DOX or Ce6 were up to 80% and 90%, respectively, indicating SF nanocapsules have a high loading capability for both cationic and anionic drugs. In vitro cell experiments proved the biocompatibility of SF nanocapsules and their burst drug release in response to acidic environment. Furthermore, chemotherapy and photodynamic therapy proved SF nanocapsules loaded with DOX or Ce6 had significant inhibition on tumor cells. Our results suggested that this LBL technique is a facile method for polymers with negative charge to fabricate nanocapsules for antitumor drug carrier. The Royal Society of Chemistry 2020-07-30 /pmc/articles/PMC9055660/ /pubmed/35519128 http://dx.doi.org/10.1039/d0ra04024j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Zheng, Heming
Duan, Bo
Xie, Zheyu
Wang, Jie
Yang, Mingying
Inventing a facile method to construct Bombyx mori (B. mori) silk fibroin nanocapsules for drug delivery
title Inventing a facile method to construct Bombyx mori (B. mori) silk fibroin nanocapsules for drug delivery
title_full Inventing a facile method to construct Bombyx mori (B. mori) silk fibroin nanocapsules for drug delivery
title_fullStr Inventing a facile method to construct Bombyx mori (B. mori) silk fibroin nanocapsules for drug delivery
title_full_unstemmed Inventing a facile method to construct Bombyx mori (B. mori) silk fibroin nanocapsules for drug delivery
title_short Inventing a facile method to construct Bombyx mori (B. mori) silk fibroin nanocapsules for drug delivery
title_sort inventing a facile method to construct bombyx mori (b. mori) silk fibroin nanocapsules for drug delivery
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055660/
https://www.ncbi.nlm.nih.gov/pubmed/35519128
http://dx.doi.org/10.1039/d0ra04024j
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