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Improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced NO

BACKGROUND: The health benefits of anthocyanins impel researchers and food producers to explorer new methods to increase anthocyanin contents in plant foods. Our previous studies revealed a positive role of nitric oxide (NO) in anthocyanin accumulation in radish (Raphanus sativus L.) sprouts. The ap...

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Autores principales: Su, Nana, Liu, Ze, Wang, Lu, Liu, Yuanyuan, Niu, Mengyang, Chen, Xin, Cui, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055698/
https://www.ncbi.nlm.nih.gov/pubmed/35490232
http://dx.doi.org/10.1186/s12870-022-03605-w
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author Su, Nana
Liu, Ze
Wang, Lu
Liu, Yuanyuan
Niu, Mengyang
Chen, Xin
Cui, Jin
author_facet Su, Nana
Liu, Ze
Wang, Lu
Liu, Yuanyuan
Niu, Mengyang
Chen, Xin
Cui, Jin
author_sort Su, Nana
collection PubMed
description BACKGROUND: The health benefits of anthocyanins impel researchers and food producers to explorer new methods to increase anthocyanin contents in plant foods. Our previous studies revealed a positive role of nitric oxide (NO) in anthocyanin accumulation in radish (Raphanus sativus L.) sprouts. The application of hemin, an inducer of heme oxygenase-1 (HO-1), can effectively elevate NO production in vivo. Hemin treatment also improves plant growth and stress tolerance. This study is aimed to assess the effects of hemin treatment on anthocyanin production in radish sprouts, and to investigate whether NO signalling is involved in this process. RESULTS: The application of hemin significantly up regulated the expressions of many anthocyanins biosynthesis related structure and regulatory genes, leading to increased anthocyanins accumulation in radish hypocotyls. Hemin treatment also raised NO contents in radish sprouts, probably through enhancing nitrate reductase (NR) activity and Nitric Oxide-Associated 1 (NOA1) expression. Comparing the effects of Zinc Protoporphyrin (ZnPP, HO-1 activity inhibitor), Sodium Nitroprusside (SNP, NO donor) and carboxy-PTIO (cPTIO, NO-scavenger) on anthocyanin and NO production, a positive role of NO signalling has been revealed in hemin-derived anthocyanin accumulation. A positive feedback loop between HO-1 and NO may be involved in regulating this process. CONCLUSIONS: Hemin induced anthocyanin accumulation in radish sprouts through HO-1 and NO signalling network. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03605-w.
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spelling pubmed-90556982022-05-01 Improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced NO Su, Nana Liu, Ze Wang, Lu Liu, Yuanyuan Niu, Mengyang Chen, Xin Cui, Jin BMC Plant Biol Research Article BACKGROUND: The health benefits of anthocyanins impel researchers and food producers to explorer new methods to increase anthocyanin contents in plant foods. Our previous studies revealed a positive role of nitric oxide (NO) in anthocyanin accumulation in radish (Raphanus sativus L.) sprouts. The application of hemin, an inducer of heme oxygenase-1 (HO-1), can effectively elevate NO production in vivo. Hemin treatment also improves plant growth and stress tolerance. This study is aimed to assess the effects of hemin treatment on anthocyanin production in radish sprouts, and to investigate whether NO signalling is involved in this process. RESULTS: The application of hemin significantly up regulated the expressions of many anthocyanins biosynthesis related structure and regulatory genes, leading to increased anthocyanins accumulation in radish hypocotyls. Hemin treatment also raised NO contents in radish sprouts, probably through enhancing nitrate reductase (NR) activity and Nitric Oxide-Associated 1 (NOA1) expression. Comparing the effects of Zinc Protoporphyrin (ZnPP, HO-1 activity inhibitor), Sodium Nitroprusside (SNP, NO donor) and carboxy-PTIO (cPTIO, NO-scavenger) on anthocyanin and NO production, a positive role of NO signalling has been revealed in hemin-derived anthocyanin accumulation. A positive feedback loop between HO-1 and NO may be involved in regulating this process. CONCLUSIONS: Hemin induced anthocyanin accumulation in radish sprouts through HO-1 and NO signalling network. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03605-w. BioMed Central 2022-04-30 /pmc/articles/PMC9055698/ /pubmed/35490232 http://dx.doi.org/10.1186/s12870-022-03605-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Su, Nana
Liu, Ze
Wang, Lu
Liu, Yuanyuan
Niu, Mengyang
Chen, Xin
Cui, Jin
Improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced NO
title Improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced NO
title_full Improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced NO
title_fullStr Improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced NO
title_full_unstemmed Improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced NO
title_short Improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced NO
title_sort improving the anthocyanin accumulation of hypocotyls in radish sprouts by hemin-induced no
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055698/
https://www.ncbi.nlm.nih.gov/pubmed/35490232
http://dx.doi.org/10.1186/s12870-022-03605-w
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