Cargando…
A highly sensitive fluorescent immunosensor for sensitive detection of nuclear matrix protein 22 as biomarker for early stage diagnosis of bladder cancer
A novel strategy is reported for highly sensitive, rapid, and selective detection of nuclear matrix protein NMP22 using two-color quantum dots based on fluorescence resonance energy transfer (FRET). Quantum dots (QDs) are highly advantageous for biological imaging and analysis, particularly when com...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055858/ https://www.ncbi.nlm.nih.gov/pubmed/35520044 http://dx.doi.org/10.1039/d0ra06191c |
_version_ | 1784697507615342592 |
---|---|
author | Othman, Hazha Omar Salehnia, Foad Fakhri, Neda Hassan, Rebwar Hosseini, Morteza Faizullah, Azad Ganjali, Mohammad Reza Kazem Aghamir, Seyed Mohammad |
author_facet | Othman, Hazha Omar Salehnia, Foad Fakhri, Neda Hassan, Rebwar Hosseini, Morteza Faizullah, Azad Ganjali, Mohammad Reza Kazem Aghamir, Seyed Mohammad |
author_sort | Othman, Hazha Omar |
collection | PubMed |
description | A novel strategy is reported for highly sensitive, rapid, and selective detection of nuclear matrix protein NMP22 using two-color quantum dots based on fluorescence resonance energy transfer (FRET). Quantum dots (QDs) are highly advantageous for biological imaging and analysis, particularly when combined with (FRET) properties of semiconductor quantum dot (QDs) are ideal for biological analysis to improve sensitivity and accuracy. In this FRET system narrowly dispersed green emitting quantum dot CdTe core is used as a donor and labelled by monoclonal (mAb) antibody, while orange emitting quantum dot CdTe/CdS core shell is used as an accepter and labelled by polyclonal (pAb) antibody. The quantum dots are labelled by antibodies using EDC/NHS as crosslinking agent. Bovine serum albumin (BSA) solution was added to block nonspecific binding sites. The fluorescence intensity of QDs accepter decreased linearly with the increasing concentrations of NMP22 from 2–22 pg mL(−1) due to FRET system and fluoroimmunoassay reaction. This method has good regression coefficient (R(2) = 0.998) and detection limit was 0.05 pg mL(−1). The proposed FRET-based immunosensor provides a quick, simple and sensitive immunoassay tool for protein detection, and can be considered as a promising approach for clinical applications. The proposed FRET-based immunosensor provides a quick, simple and sensitive immunoassay tool for protein detection, and can be considered as a promising approach for clinical applications. |
format | Online Article Text |
id | pubmed-9055858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90558582022-05-04 A highly sensitive fluorescent immunosensor for sensitive detection of nuclear matrix protein 22 as biomarker for early stage diagnosis of bladder cancer Othman, Hazha Omar Salehnia, Foad Fakhri, Neda Hassan, Rebwar Hosseini, Morteza Faizullah, Azad Ganjali, Mohammad Reza Kazem Aghamir, Seyed Mohammad RSC Adv Chemistry A novel strategy is reported for highly sensitive, rapid, and selective detection of nuclear matrix protein NMP22 using two-color quantum dots based on fluorescence resonance energy transfer (FRET). Quantum dots (QDs) are highly advantageous for biological imaging and analysis, particularly when combined with (FRET) properties of semiconductor quantum dot (QDs) are ideal for biological analysis to improve sensitivity and accuracy. In this FRET system narrowly dispersed green emitting quantum dot CdTe core is used as a donor and labelled by monoclonal (mAb) antibody, while orange emitting quantum dot CdTe/CdS core shell is used as an accepter and labelled by polyclonal (pAb) antibody. The quantum dots are labelled by antibodies using EDC/NHS as crosslinking agent. Bovine serum albumin (BSA) solution was added to block nonspecific binding sites. The fluorescence intensity of QDs accepter decreased linearly with the increasing concentrations of NMP22 from 2–22 pg mL(−1) due to FRET system and fluoroimmunoassay reaction. This method has good regression coefficient (R(2) = 0.998) and detection limit was 0.05 pg mL(−1). The proposed FRET-based immunosensor provides a quick, simple and sensitive immunoassay tool for protein detection, and can be considered as a promising approach for clinical applications. The proposed FRET-based immunosensor provides a quick, simple and sensitive immunoassay tool for protein detection, and can be considered as a promising approach for clinical applications. The Royal Society of Chemistry 2020-08-04 /pmc/articles/PMC9055858/ /pubmed/35520044 http://dx.doi.org/10.1039/d0ra06191c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Othman, Hazha Omar Salehnia, Foad Fakhri, Neda Hassan, Rebwar Hosseini, Morteza Faizullah, Azad Ganjali, Mohammad Reza Kazem Aghamir, Seyed Mohammad A highly sensitive fluorescent immunosensor for sensitive detection of nuclear matrix protein 22 as biomarker for early stage diagnosis of bladder cancer |
title | A highly sensitive fluorescent immunosensor for sensitive detection of nuclear matrix protein 22 as biomarker for early stage diagnosis of bladder cancer |
title_full | A highly sensitive fluorescent immunosensor for sensitive detection of nuclear matrix protein 22 as biomarker for early stage diagnosis of bladder cancer |
title_fullStr | A highly sensitive fluorescent immunosensor for sensitive detection of nuclear matrix protein 22 as biomarker for early stage diagnosis of bladder cancer |
title_full_unstemmed | A highly sensitive fluorescent immunosensor for sensitive detection of nuclear matrix protein 22 as biomarker for early stage diagnosis of bladder cancer |
title_short | A highly sensitive fluorescent immunosensor for sensitive detection of nuclear matrix protein 22 as biomarker for early stage diagnosis of bladder cancer |
title_sort | highly sensitive fluorescent immunosensor for sensitive detection of nuclear matrix protein 22 as biomarker for early stage diagnosis of bladder cancer |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055858/ https://www.ncbi.nlm.nih.gov/pubmed/35520044 http://dx.doi.org/10.1039/d0ra06191c |
work_keys_str_mv | AT othmanhazhaomar ahighlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT salehniafoad ahighlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT fakhrineda ahighlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT hassanrebwar ahighlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT hosseinimorteza ahighlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT faizullahazad ahighlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT ganjalimohammadreza ahighlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT kazemaghamirseyedmohammad ahighlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT othmanhazhaomar highlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT salehniafoad highlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT fakhrineda highlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT hassanrebwar highlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT hosseinimorteza highlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT faizullahazad highlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT ganjalimohammadreza highlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer AT kazemaghamirseyedmohammad highlysensitivefluorescentimmunosensorforsensitivedetectionofnuclearmatrixprotein22asbiomarkerforearlystagediagnosisofbladdercancer |