Neuroprotective constituents from the aerial parts of Cannabis sativa L. subsp. sativa

Five new compounds including three new cannabinoids, cannabisativas A–C (1–3), two new phenolic acids, (7Z,9Z)-cannabiphenolic acid A (4) and (8S,9Z)-cannabiphenolic acid B (5), together with twelve known compounds (6–17), were isolated from the aerial parts of Cannabis sativa L. subsp. sativa. The structures of 1–5 were established on the basis of extensive 1D, 2D NMR and HRESIMS analysis. The absolute configurations were determined by comparison between their experimental and calculated spectra of electronic circular dichroism (ECD) or the modified Mosher's method. The neuroprotective effects of the compounds 1–17 were evaluated on PC 12 cells. Compounds 12, 13 and 15 showed potential protective effects against H(2)O(2)-induced damage.