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An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection

Nucleic acids have special ability to organize themselves into various non-canonical structures, including a four-stranded DNA structure termed G-quadruplex (G4) that has been utilized for diagnostic and therapeutic applications. Herein, we report the ability of G4 to distinguish dengue virus (DENV)...

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Autores principales: Ida, Jeunice, Kuzuya, Akinori, Choong, Yee Siew, Lim, Theam Soon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9056686/
https://www.ncbi.nlm.nih.gov/pubmed/35515051
http://dx.doi.org/10.1039/d0ra05439a
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author Ida, Jeunice
Kuzuya, Akinori
Choong, Yee Siew
Lim, Theam Soon
author_facet Ida, Jeunice
Kuzuya, Akinori
Choong, Yee Siew
Lim, Theam Soon
author_sort Ida, Jeunice
collection PubMed
description Nucleic acids have special ability to organize themselves into various non-canonical structures, including a four-stranded DNA structure termed G-quadruplex (G4) that has been utilized for diagnostic and therapeutic applications. Herein, we report the ability of G4 to distinguish dengue virus (DENV) based on its serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) using a split G4-hemin DNAzyme configuration. In this system, two separate G-rich oligonucleotides are brought together upon target DNA strand hybridization to form a three-way junction architecture, allowing the formation of a G4 structure. The G4 formation in complexation with hemin can thus provide a signal readout by generating a DNAzyme that is able to catalyze H(2)O(2)-mediated oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). This results in a change of color providing a sensing platform for the colorimetric detection of DENV. In our approach, betaine and dimethyl sulfoxide were utilized for better G4 generation by enhancing the target-probe hybridization. In addition to this serotype-specific assay, a multi-probe cocktail assay, which is an all-in-one assay was also examined for DENV detection. The system highlights the potential of split G-quadruplex configurations for the development of DNA-based detection and serotyping systems in the future.
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spelling pubmed-90566862022-05-04 An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection Ida, Jeunice Kuzuya, Akinori Choong, Yee Siew Lim, Theam Soon RSC Adv Chemistry Nucleic acids have special ability to organize themselves into various non-canonical structures, including a four-stranded DNA structure termed G-quadruplex (G4) that has been utilized for diagnostic and therapeutic applications. Herein, we report the ability of G4 to distinguish dengue virus (DENV) based on its serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) using a split G4-hemin DNAzyme configuration. In this system, two separate G-rich oligonucleotides are brought together upon target DNA strand hybridization to form a three-way junction architecture, allowing the formation of a G4 structure. The G4 formation in complexation with hemin can thus provide a signal readout by generating a DNAzyme that is able to catalyze H(2)O(2)-mediated oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). This results in a change of color providing a sensing platform for the colorimetric detection of DENV. In our approach, betaine and dimethyl sulfoxide were utilized for better G4 generation by enhancing the target-probe hybridization. In addition to this serotype-specific assay, a multi-probe cocktail assay, which is an all-in-one assay was also examined for DENV detection. The system highlights the potential of split G-quadruplex configurations for the development of DNA-based detection and serotyping systems in the future. The Royal Society of Chemistry 2020-09-07 /pmc/articles/PMC9056686/ /pubmed/35515051 http://dx.doi.org/10.1039/d0ra05439a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Ida, Jeunice
Kuzuya, Akinori
Choong, Yee Siew
Lim, Theam Soon
An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection
title An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection
title_full An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection
title_fullStr An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection
title_full_unstemmed An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection
title_short An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection
title_sort intermolecular-split g-quadruplex dnazyme sensor for dengue virus detection
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9056686/
https://www.ncbi.nlm.nih.gov/pubmed/35515051
http://dx.doi.org/10.1039/d0ra05439a
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