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An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection
Nucleic acids have special ability to organize themselves into various non-canonical structures, including a four-stranded DNA structure termed G-quadruplex (G4) that has been utilized for diagnostic and therapeutic applications. Herein, we report the ability of G4 to distinguish dengue virus (DENV)...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9056686/ https://www.ncbi.nlm.nih.gov/pubmed/35515051 http://dx.doi.org/10.1039/d0ra05439a |
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author | Ida, Jeunice Kuzuya, Akinori Choong, Yee Siew Lim, Theam Soon |
author_facet | Ida, Jeunice Kuzuya, Akinori Choong, Yee Siew Lim, Theam Soon |
author_sort | Ida, Jeunice |
collection | PubMed |
description | Nucleic acids have special ability to organize themselves into various non-canonical structures, including a four-stranded DNA structure termed G-quadruplex (G4) that has been utilized for diagnostic and therapeutic applications. Herein, we report the ability of G4 to distinguish dengue virus (DENV) based on its serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) using a split G4-hemin DNAzyme configuration. In this system, two separate G-rich oligonucleotides are brought together upon target DNA strand hybridization to form a three-way junction architecture, allowing the formation of a G4 structure. The G4 formation in complexation with hemin can thus provide a signal readout by generating a DNAzyme that is able to catalyze H(2)O(2)-mediated oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). This results in a change of color providing a sensing platform for the colorimetric detection of DENV. In our approach, betaine and dimethyl sulfoxide were utilized for better G4 generation by enhancing the target-probe hybridization. In addition to this serotype-specific assay, a multi-probe cocktail assay, which is an all-in-one assay was also examined for DENV detection. The system highlights the potential of split G-quadruplex configurations for the development of DNA-based detection and serotyping systems in the future. |
format | Online Article Text |
id | pubmed-9056686 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90566862022-05-04 An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection Ida, Jeunice Kuzuya, Akinori Choong, Yee Siew Lim, Theam Soon RSC Adv Chemistry Nucleic acids have special ability to organize themselves into various non-canonical structures, including a four-stranded DNA structure termed G-quadruplex (G4) that has been utilized for diagnostic and therapeutic applications. Herein, we report the ability of G4 to distinguish dengue virus (DENV) based on its serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) using a split G4-hemin DNAzyme configuration. In this system, two separate G-rich oligonucleotides are brought together upon target DNA strand hybridization to form a three-way junction architecture, allowing the formation of a G4 structure. The G4 formation in complexation with hemin can thus provide a signal readout by generating a DNAzyme that is able to catalyze H(2)O(2)-mediated oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). This results in a change of color providing a sensing platform for the colorimetric detection of DENV. In our approach, betaine and dimethyl sulfoxide were utilized for better G4 generation by enhancing the target-probe hybridization. In addition to this serotype-specific assay, a multi-probe cocktail assay, which is an all-in-one assay was also examined for DENV detection. The system highlights the potential of split G-quadruplex configurations for the development of DNA-based detection and serotyping systems in the future. The Royal Society of Chemistry 2020-09-07 /pmc/articles/PMC9056686/ /pubmed/35515051 http://dx.doi.org/10.1039/d0ra05439a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Ida, Jeunice Kuzuya, Akinori Choong, Yee Siew Lim, Theam Soon An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection |
title | An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection |
title_full | An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection |
title_fullStr | An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection |
title_full_unstemmed | An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection |
title_short | An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection |
title_sort | intermolecular-split g-quadruplex dnazyme sensor for dengue virus detection |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9056686/ https://www.ncbi.nlm.nih.gov/pubmed/35515051 http://dx.doi.org/10.1039/d0ra05439a |
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