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Superparamagnetic iron oxide nanoparticles (SPIONs) conjugated with lipase Candida antarctica A for biodiesel synthesis

Biodiesel is an alternative biodegradable and non-toxic fuel, with a low emission profile and capable of reducing significantly the level of carcinogenic pollutants released into the atmosphere. A newly designed nano-biocatalyst prepared by conjugation of lipase A on superparamagnetic iron oxide nan...

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Autores principales: Peffi Ferreira, Luis Fernando, Mazzi de Oliveira, Thayná, Toma, Sergio Hiroshi, Toyama, Marcos Makoto, Araki, Koiti, Avanzi, Luis Humberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9057248/
https://www.ncbi.nlm.nih.gov/pubmed/35517526
http://dx.doi.org/10.1039/d0ra06215d
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author Peffi Ferreira, Luis Fernando
Mazzi de Oliveira, Thayná
Toma, Sergio Hiroshi
Toyama, Marcos Makoto
Araki, Koiti
Avanzi, Luis Humberto
author_facet Peffi Ferreira, Luis Fernando
Mazzi de Oliveira, Thayná
Toma, Sergio Hiroshi
Toyama, Marcos Makoto
Araki, Koiti
Avanzi, Luis Humberto
author_sort Peffi Ferreira, Luis Fernando
collection PubMed
description Biodiesel is an alternative biodegradable and non-toxic fuel, with a low emission profile and capable of reducing significantly the level of carcinogenic pollutants released into the atmosphere. A newly designed nano-biocatalyst prepared by conjugation of lipase A on superparamagnetic iron oxide nanoparticles (SPIONs) demonstrated high efficiency for production of biodiesel by the reaction of soybean oil with anhydrous methanol. The nanomaterial was characterized by FTIR, TGA and XRD, and its enzymatic activity compared with Lipozyme 435, a commercial gold standard from Novozyme™, which presented average enzymatic activity of 4559 ± 75 only twice as large as that of the SPION-CAL-A catalyst (2283 ± 249 PLU g(−1)), whereas Lipozyme TLIM showed a much lower activity of 588 ± 16 PLU g(−1). These results were confirmed in the transesterification reaction for production of biodiesel where a yield of 11.4% was achieved with Lipozyme 435 and 4.6 ± 0.5% with the nano-biocatalyst. Such an improved performance associated with easy magnetic recovery and reuse make the material potentially interesting for production of biodiesel from used cooking oil, adding value to this abundant resource.
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spelling pubmed-90572482022-05-04 Superparamagnetic iron oxide nanoparticles (SPIONs) conjugated with lipase Candida antarctica A for biodiesel synthesis Peffi Ferreira, Luis Fernando Mazzi de Oliveira, Thayná Toma, Sergio Hiroshi Toyama, Marcos Makoto Araki, Koiti Avanzi, Luis Humberto RSC Adv Chemistry Biodiesel is an alternative biodegradable and non-toxic fuel, with a low emission profile and capable of reducing significantly the level of carcinogenic pollutants released into the atmosphere. A newly designed nano-biocatalyst prepared by conjugation of lipase A on superparamagnetic iron oxide nanoparticles (SPIONs) demonstrated high efficiency for production of biodiesel by the reaction of soybean oil with anhydrous methanol. The nanomaterial was characterized by FTIR, TGA and XRD, and its enzymatic activity compared with Lipozyme 435, a commercial gold standard from Novozyme™, which presented average enzymatic activity of 4559 ± 75 only twice as large as that of the SPION-CAL-A catalyst (2283 ± 249 PLU g(−1)), whereas Lipozyme TLIM showed a much lower activity of 588 ± 16 PLU g(−1). These results were confirmed in the transesterification reaction for production of biodiesel where a yield of 11.4% was achieved with Lipozyme 435 and 4.6 ± 0.5% with the nano-biocatalyst. Such an improved performance associated with easy magnetic recovery and reuse make the material potentially interesting for production of biodiesel from used cooking oil, adding value to this abundant resource. The Royal Society of Chemistry 2020-10-20 /pmc/articles/PMC9057248/ /pubmed/35517526 http://dx.doi.org/10.1039/d0ra06215d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Peffi Ferreira, Luis Fernando
Mazzi de Oliveira, Thayná
Toma, Sergio Hiroshi
Toyama, Marcos Makoto
Araki, Koiti
Avanzi, Luis Humberto
Superparamagnetic iron oxide nanoparticles (SPIONs) conjugated with lipase Candida antarctica A for biodiesel synthesis
title Superparamagnetic iron oxide nanoparticles (SPIONs) conjugated with lipase Candida antarctica A for biodiesel synthesis
title_full Superparamagnetic iron oxide nanoparticles (SPIONs) conjugated with lipase Candida antarctica A for biodiesel synthesis
title_fullStr Superparamagnetic iron oxide nanoparticles (SPIONs) conjugated with lipase Candida antarctica A for biodiesel synthesis
title_full_unstemmed Superparamagnetic iron oxide nanoparticles (SPIONs) conjugated with lipase Candida antarctica A for biodiesel synthesis
title_short Superparamagnetic iron oxide nanoparticles (SPIONs) conjugated with lipase Candida antarctica A for biodiesel synthesis
title_sort superparamagnetic iron oxide nanoparticles (spions) conjugated with lipase candida antarctica a for biodiesel synthesis
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9057248/
https://www.ncbi.nlm.nih.gov/pubmed/35517526
http://dx.doi.org/10.1039/d0ra06215d
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