Cargando…
Anti-inflammatory activity of a water-soluble polysaccharide from the roots of purple sweet potato
In this study, a water-soluble polysaccharide was isolated from purple sweet potato roots. The in vitro and in vivo anti-inflammatory effects of the polysaccharide were evaluated by lipopolysaccharide (LPS)-induced inflammatory RAW264.7 macrophages and mice, respectively. The in vitro anti-inflammat...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9057464/ https://www.ncbi.nlm.nih.gov/pubmed/35515390 http://dx.doi.org/10.1039/d0ra07551e |
_version_ | 1784697902391623680 |
---|---|
author | Sun, Jian Gou, Yarun Liu, Jun Chen, Hong Kan, Juan Qian, Chunlu Zhang, Nianfeng Niu, Fuxiang Jin, Changhai |
author_facet | Sun, Jian Gou, Yarun Liu, Jun Chen, Hong Kan, Juan Qian, Chunlu Zhang, Nianfeng Niu, Fuxiang Jin, Changhai |
author_sort | Sun, Jian |
collection | PubMed |
description | In this study, a water-soluble polysaccharide was isolated from purple sweet potato roots. The in vitro and in vivo anti-inflammatory effects of the polysaccharide were evaluated by lipopolysaccharide (LPS)-induced inflammatory RAW264.7 macrophages and mice, respectively. The in vitro anti-inflammatory assay showed that the polysaccharide could effectively inhibit the overproduction of nitric oxide and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) while increasing the secretion of anti-inflammatory cytokine (IL-10). The in vivo anti-inflammatory assay revealed that mice administered with the polysaccharide showed higher IL-10, SOD, and T-AOC levels but lower TNF-α, IL-1β, IL-6 and MDA levels as compared to the LPS-treated model. Meanwhile, mice administered with the polysaccharide showed increased abundance of Lachnospiraceae, Lactobacillales and Parabacteroides but decreased amounts of Psychrobacter and Staphylococcus as compared to the LPS model group. Moreover, mice administered with polysaccharide showed enhanced production of short chain fatty acids by gut microbiota in the lipopolysaccharide-induced inflammatory mice. Our results suggested that the water-soluble polysaccharide from purple sweet potato roots could be utilized as a novel anti-inflammatory agent. |
format | Online Article Text |
id | pubmed-9057464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90574642022-05-04 Anti-inflammatory activity of a water-soluble polysaccharide from the roots of purple sweet potato Sun, Jian Gou, Yarun Liu, Jun Chen, Hong Kan, Juan Qian, Chunlu Zhang, Nianfeng Niu, Fuxiang Jin, Changhai RSC Adv Chemistry In this study, a water-soluble polysaccharide was isolated from purple sweet potato roots. The in vitro and in vivo anti-inflammatory effects of the polysaccharide were evaluated by lipopolysaccharide (LPS)-induced inflammatory RAW264.7 macrophages and mice, respectively. The in vitro anti-inflammatory assay showed that the polysaccharide could effectively inhibit the overproduction of nitric oxide and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) while increasing the secretion of anti-inflammatory cytokine (IL-10). The in vivo anti-inflammatory assay revealed that mice administered with the polysaccharide showed higher IL-10, SOD, and T-AOC levels but lower TNF-α, IL-1β, IL-6 and MDA levels as compared to the LPS-treated model. Meanwhile, mice administered with the polysaccharide showed increased abundance of Lachnospiraceae, Lactobacillales and Parabacteroides but decreased amounts of Psychrobacter and Staphylococcus as compared to the LPS model group. Moreover, mice administered with polysaccharide showed enhanced production of short chain fatty acids by gut microbiota in the lipopolysaccharide-induced inflammatory mice. Our results suggested that the water-soluble polysaccharide from purple sweet potato roots could be utilized as a novel anti-inflammatory agent. The Royal Society of Chemistry 2020-10-29 /pmc/articles/PMC9057464/ /pubmed/35515390 http://dx.doi.org/10.1039/d0ra07551e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Sun, Jian Gou, Yarun Liu, Jun Chen, Hong Kan, Juan Qian, Chunlu Zhang, Nianfeng Niu, Fuxiang Jin, Changhai Anti-inflammatory activity of a water-soluble polysaccharide from the roots of purple sweet potato |
title | Anti-inflammatory activity of a water-soluble polysaccharide from the roots of purple sweet potato |
title_full | Anti-inflammatory activity of a water-soluble polysaccharide from the roots of purple sweet potato |
title_fullStr | Anti-inflammatory activity of a water-soluble polysaccharide from the roots of purple sweet potato |
title_full_unstemmed | Anti-inflammatory activity of a water-soluble polysaccharide from the roots of purple sweet potato |
title_short | Anti-inflammatory activity of a water-soluble polysaccharide from the roots of purple sweet potato |
title_sort | anti-inflammatory activity of a water-soluble polysaccharide from the roots of purple sweet potato |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9057464/ https://www.ncbi.nlm.nih.gov/pubmed/35515390 http://dx.doi.org/10.1039/d0ra07551e |
work_keys_str_mv | AT sunjian antiinflammatoryactivityofawatersolublepolysaccharidefromtherootsofpurplesweetpotato AT gouyarun antiinflammatoryactivityofawatersolublepolysaccharidefromtherootsofpurplesweetpotato AT liujun antiinflammatoryactivityofawatersolublepolysaccharidefromtherootsofpurplesweetpotato AT chenhong antiinflammatoryactivityofawatersolublepolysaccharidefromtherootsofpurplesweetpotato AT kanjuan antiinflammatoryactivityofawatersolublepolysaccharidefromtherootsofpurplesweetpotato AT qianchunlu antiinflammatoryactivityofawatersolublepolysaccharidefromtherootsofpurplesweetpotato AT zhangnianfeng antiinflammatoryactivityofawatersolublepolysaccharidefromtherootsofpurplesweetpotato AT niufuxiang antiinflammatoryactivityofawatersolublepolysaccharidefromtherootsofpurplesweetpotato AT jinchanghai antiinflammatoryactivityofawatersolublepolysaccharidefromtherootsofpurplesweetpotato |