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Temperature-responsive iron nanozymes based on poly(N-vinylcaprolactam) with multi-enzyme activity

Iron (Fe)-based nanozymes are widely applied in the biomedical field due to their enzyme-like catalytic activity. Herein, Fe(ii)-based coordination polymer nanohydrogels (FeCPNGs) have been conveniently prepared as a new type of nanozyme by the chelation reaction between ferrous iron and polymer nan...

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Detalles Bibliográficos
Autores principales: Wang, Yang, Wang, Wei, Gu, Zhun, Miao, Xiangyang, Huang, Qiuyan, Chang, Baisong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9057499/
https://www.ncbi.nlm.nih.gov/pubmed/35515401
http://dx.doi.org/10.1039/d0ra07226e
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author Wang, Yang
Wang, Wei
Gu, Zhun
Miao, Xiangyang
Huang, Qiuyan
Chang, Baisong
author_facet Wang, Yang
Wang, Wei
Gu, Zhun
Miao, Xiangyang
Huang, Qiuyan
Chang, Baisong
author_sort Wang, Yang
collection PubMed
description Iron (Fe)-based nanozymes are widely applied in the biomedical field due to their enzyme-like catalytic activity. Herein, Fe(ii)-based coordination polymer nanohydrogels (FeCPNGs) have been conveniently prepared as a new type of nanozyme by the chelation reaction between ferrous iron and polymer nanohydrogels. The P(VCL-co-NMAM) nanohydrogels prepared by a reflux precipitation polymerization method using N-vinylcaprolactam (VCL) and N-methylol acrylamide (NMAM) as monomers and N,N-methylenebisacrylamide (MBA) as a crosslinker were esterified using P(2)O(5) and then chelated with Fe(ii) ions to form nanozymes with peroxidase and superoxide dismutase (SOD) activity. It was found by dynamic light scattering (DLS) and transmission electron microscopy (TEM) that the nanohydrogels prepared with a monomer concentration of 4% and mass ratio of 1 : 1 (VCL : NMAM) had more uniform particle size, better dispersion and a distinct temperature response. The results of Fourier transform infrared (FTIR), DLS, TEM, X-ray powder diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) indicated the successful preparation of the esterified nanohydrogel and FeCPNGs. Of particular importance is that such FeCPNGs can functionally mimic two antioxidant enzymes (peroxidase and superoxide dismutase) by UV analysis of catalytic oxidation between 3,3,5,5-tetramethylbenzidine (TMB) and H(2)O(2) and the kit analysis of SOD-like activity.
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spelling pubmed-90574992022-05-04 Temperature-responsive iron nanozymes based on poly(N-vinylcaprolactam) with multi-enzyme activity Wang, Yang Wang, Wei Gu, Zhun Miao, Xiangyang Huang, Qiuyan Chang, Baisong RSC Adv Chemistry Iron (Fe)-based nanozymes are widely applied in the biomedical field due to their enzyme-like catalytic activity. Herein, Fe(ii)-based coordination polymer nanohydrogels (FeCPNGs) have been conveniently prepared as a new type of nanozyme by the chelation reaction between ferrous iron and polymer nanohydrogels. The P(VCL-co-NMAM) nanohydrogels prepared by a reflux precipitation polymerization method using N-vinylcaprolactam (VCL) and N-methylol acrylamide (NMAM) as monomers and N,N-methylenebisacrylamide (MBA) as a crosslinker were esterified using P(2)O(5) and then chelated with Fe(ii) ions to form nanozymes with peroxidase and superoxide dismutase (SOD) activity. It was found by dynamic light scattering (DLS) and transmission electron microscopy (TEM) that the nanohydrogels prepared with a monomer concentration of 4% and mass ratio of 1 : 1 (VCL : NMAM) had more uniform particle size, better dispersion and a distinct temperature response. The results of Fourier transform infrared (FTIR), DLS, TEM, X-ray powder diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) indicated the successful preparation of the esterified nanohydrogel and FeCPNGs. Of particular importance is that such FeCPNGs can functionally mimic two antioxidant enzymes (peroxidase and superoxide dismutase) by UV analysis of catalytic oxidation between 3,3,5,5-tetramethylbenzidine (TMB) and H(2)O(2) and the kit analysis of SOD-like activity. The Royal Society of Chemistry 2020-11-02 /pmc/articles/PMC9057499/ /pubmed/35515401 http://dx.doi.org/10.1039/d0ra07226e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Wang, Yang
Wang, Wei
Gu, Zhun
Miao, Xiangyang
Huang, Qiuyan
Chang, Baisong
Temperature-responsive iron nanozymes based on poly(N-vinylcaprolactam) with multi-enzyme activity
title Temperature-responsive iron nanozymes based on poly(N-vinylcaprolactam) with multi-enzyme activity
title_full Temperature-responsive iron nanozymes based on poly(N-vinylcaprolactam) with multi-enzyme activity
title_fullStr Temperature-responsive iron nanozymes based on poly(N-vinylcaprolactam) with multi-enzyme activity
title_full_unstemmed Temperature-responsive iron nanozymes based on poly(N-vinylcaprolactam) with multi-enzyme activity
title_short Temperature-responsive iron nanozymes based on poly(N-vinylcaprolactam) with multi-enzyme activity
title_sort temperature-responsive iron nanozymes based on poly(n-vinylcaprolactam) with multi-enzyme activity
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9057499/
https://www.ncbi.nlm.nih.gov/pubmed/35515401
http://dx.doi.org/10.1039/d0ra07226e
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