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Development of micropillar array electrodes for highly sensitive detection of biomarkers

Micropillar array electrodes (μAEs) have been widely applied in electrochemical detection owing to their advantages of increased mass transport, lower detection limit, and potential to be miniaturized. This paper reports the fabrication, simulation, surface modification, and characterization of PDMS...

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Autores principales: Chen, Chaozhan, Ran, Bin, Wang, Zhenxing, Zhao, Hongli, Lan, Minbo, Chen, Huaying, Zhu, Yonggang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9057762/
https://www.ncbi.nlm.nih.gov/pubmed/35519230
http://dx.doi.org/10.1039/d0ra07694e
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author Chen, Chaozhan
Ran, Bin
Wang, Zhenxing
Zhao, Hongli
Lan, Minbo
Chen, Huaying
Zhu, Yonggang
author_facet Chen, Chaozhan
Ran, Bin
Wang, Zhenxing
Zhao, Hongli
Lan, Minbo
Chen, Huaying
Zhu, Yonggang
author_sort Chen, Chaozhan
collection PubMed
description Micropillar array electrodes (μAEs) have been widely applied in electrochemical detection owing to their advantages of increased mass transport, lower detection limit, and potential to be miniaturized. This paper reports the fabrication, simulation, surface modification, and characterization of PDMS-based μAEs coated with gold films. The μAEs consist of 9 × 10 micropillars with a height of either 100 μm, 300 μm, or 500 μm in a 0.09 cm(2) region. Numerical simulation was employed to study the influence of geometrical parameters on the current density. The μAEs were fabricated by soft lithography and characterized using both SEM and cyclic voltammetry. Experiments revealed that high pillars enabled enhanced voltammetric current density regardless of the scan rates. The platinum–palladium/multi-walled carbon nanotubes (Pt–Pd/MWCNTs) were coated on the μAEs to improve their electrochemical detection capability. The μAEs demonstrated 1.5 times larger sensitivity compared with the planar electrode when hydrogen peroxide was detected. Furthermore, μAE500 with Pt–Pd/MWCNTs was employed to detect sarcosine, a potential biomarker for prostate cancer. The linear range and limit of detection for sarcosine were from 5 to 60 μM and 1.28 μM, respectively. This detection range covers the concentration of sarcosine in human tissues (0–60 μM). These results suggest that the μAEs have better detection performance in comparison to planar electrodes due to their large surface area and pillar height. This paper provides essential guidelines for the application of μAEs in high sensitivity electrochemical detection of low abundance analytes.
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spelling pubmed-90577622022-05-04 Development of micropillar array electrodes for highly sensitive detection of biomarkers Chen, Chaozhan Ran, Bin Wang, Zhenxing Zhao, Hongli Lan, Minbo Chen, Huaying Zhu, Yonggang RSC Adv Chemistry Micropillar array electrodes (μAEs) have been widely applied in electrochemical detection owing to their advantages of increased mass transport, lower detection limit, and potential to be miniaturized. This paper reports the fabrication, simulation, surface modification, and characterization of PDMS-based μAEs coated with gold films. The μAEs consist of 9 × 10 micropillars with a height of either 100 μm, 300 μm, or 500 μm in a 0.09 cm(2) region. Numerical simulation was employed to study the influence of geometrical parameters on the current density. The μAEs were fabricated by soft lithography and characterized using both SEM and cyclic voltammetry. Experiments revealed that high pillars enabled enhanced voltammetric current density regardless of the scan rates. The platinum–palladium/multi-walled carbon nanotubes (Pt–Pd/MWCNTs) were coated on the μAEs to improve their electrochemical detection capability. The μAEs demonstrated 1.5 times larger sensitivity compared with the planar electrode when hydrogen peroxide was detected. Furthermore, μAE500 with Pt–Pd/MWCNTs was employed to detect sarcosine, a potential biomarker for prostate cancer. The linear range and limit of detection for sarcosine were from 5 to 60 μM and 1.28 μM, respectively. This detection range covers the concentration of sarcosine in human tissues (0–60 μM). These results suggest that the μAEs have better detection performance in comparison to planar electrodes due to their large surface area and pillar height. This paper provides essential guidelines for the application of μAEs in high sensitivity electrochemical detection of low abundance analytes. The Royal Society of Chemistry 2020-11-10 /pmc/articles/PMC9057762/ /pubmed/35519230 http://dx.doi.org/10.1039/d0ra07694e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Chen, Chaozhan
Ran, Bin
Wang, Zhenxing
Zhao, Hongli
Lan, Minbo
Chen, Huaying
Zhu, Yonggang
Development of micropillar array electrodes for highly sensitive detection of biomarkers
title Development of micropillar array electrodes for highly sensitive detection of biomarkers
title_full Development of micropillar array electrodes for highly sensitive detection of biomarkers
title_fullStr Development of micropillar array electrodes for highly sensitive detection of biomarkers
title_full_unstemmed Development of micropillar array electrodes for highly sensitive detection of biomarkers
title_short Development of micropillar array electrodes for highly sensitive detection of biomarkers
title_sort development of micropillar array electrodes for highly sensitive detection of biomarkers
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9057762/
https://www.ncbi.nlm.nih.gov/pubmed/35519230
http://dx.doi.org/10.1039/d0ra07694e
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