An antibody-based proximity labeling protocol to identify biotinylated interactors of SARS-CoV-2

Elucidating the molecular interactions between virus and host is fundamental to understanding the mechanism of viral pathogenesis. Here, we present a protocol to screen SARS-CoV-2 protein interactors using an antibody-based TurboID proximity labeling approach. This technique directly identifies biot...

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Detalles Bibliográficos
Autores principales: Shang, Limin, Zhang, Yuehui, Liu, Yuchen, Jin, Chaozhi, Zhao, Yanan, Zhang, Jing, Wang, Pei-Hui, Wang, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9057979/
https://www.ncbi.nlm.nih.gov/pubmed/35611119
http://dx.doi.org/10.1016/j.xpro.2022.101406

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