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Investigation of HPLF-111624 in Modified Experimental Models of Ulcerative Proctitis and Anal Fissure in Rats

BACKGROUND: Although ulcerative proctitis (UP) and anal fissure (AF) are common anorectal diseases, there are no appropriate experimental models to screen the drugs intended for these conditions. In this context, existing experimental models mimicking these diseases were modified and the polyherbal...

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Autores principales: Onkaramurthy, Mallappa, Azeemuddin, Mohammed Mukhram, Baig, Mirza Rizwan, Kendaganna, Pavan Heggadadevanakote, Rafiq, Mohamed, Babu, Uddagiri Venkanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058020/
https://www.ncbi.nlm.nih.gov/pubmed/35509969
http://dx.doi.org/10.2147/JEP.S345599
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author Onkaramurthy, Mallappa
Azeemuddin, Mohammed Mukhram
Baig, Mirza Rizwan
Kendaganna, Pavan Heggadadevanakote
Rafiq, Mohamed
Babu, Uddagiri Venkanna
author_facet Onkaramurthy, Mallappa
Azeemuddin, Mohammed Mukhram
Baig, Mirza Rizwan
Kendaganna, Pavan Heggadadevanakote
Rafiq, Mohamed
Babu, Uddagiri Venkanna
author_sort Onkaramurthy, Mallappa
collection PubMed
description BACKGROUND: Although ulcerative proctitis (UP) and anal fissure (AF) are common anorectal diseases, there are no appropriate experimental models to screen the drugs intended for these conditions. In this context, existing experimental models mimicking these diseases were modified and the polyherbal formulation, HPLF-111624 was evaluated in these models. OBJECTIVE: To establish animal model for UP and AF and to evaluate polyherbal formulation, HPLF-111624 in these disease models. METHODS: An experimental model of UP was selected based on the modification of the ulcerative colitis model using different concentrations of acetic acid. The concentration used for induction were 2.5%, 5% and 10% v/v and different weights used to induce AF were 25 g, 50 g and 100 g, which were selected based on the severity of inflammation, fecal score, gross pathology, and histopathological evaluation. Furthermore, these animal models were used to evaluate the efficacy of HPLF-111624, a polyherbal formulation known to be beneficial in anal diseases. RESULTS: Acetic acid at 5% produced typical pathological changes that resembled UP, with a significant increase in the fecal score, gross lesion, and histopathological changes. Similarly, among the three weights, physical injury with a 100 g weight produced significant changes in the histopathological score in the model of AF. Intervention with HPLF-111624 at doses of 250 and 500 mg/kg b.wt., showed a reduction in the inflammatory cytokines and a significant improvement in the histopathological findings in both the conditions. CONCLUSION: The results showed that the modified experimental models for UP and AF resemble the human pathological conditions and are simple, versatile and may be used for screening drugs intended for these conditions. Intervention with HPLF-111624 was found to be effective in improving the pathological state of UP and AF.
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spelling pubmed-90580202022-05-03 Investigation of HPLF-111624 in Modified Experimental Models of Ulcerative Proctitis and Anal Fissure in Rats Onkaramurthy, Mallappa Azeemuddin, Mohammed Mukhram Baig, Mirza Rizwan Kendaganna, Pavan Heggadadevanakote Rafiq, Mohamed Babu, Uddagiri Venkanna J Exp Pharmacol Original Research BACKGROUND: Although ulcerative proctitis (UP) and anal fissure (AF) are common anorectal diseases, there are no appropriate experimental models to screen the drugs intended for these conditions. In this context, existing experimental models mimicking these diseases were modified and the polyherbal formulation, HPLF-111624 was evaluated in these models. OBJECTIVE: To establish animal model for UP and AF and to evaluate polyherbal formulation, HPLF-111624 in these disease models. METHODS: An experimental model of UP was selected based on the modification of the ulcerative colitis model using different concentrations of acetic acid. The concentration used for induction were 2.5%, 5% and 10% v/v and different weights used to induce AF were 25 g, 50 g and 100 g, which were selected based on the severity of inflammation, fecal score, gross pathology, and histopathological evaluation. Furthermore, these animal models were used to evaluate the efficacy of HPLF-111624, a polyherbal formulation known to be beneficial in anal diseases. RESULTS: Acetic acid at 5% produced typical pathological changes that resembled UP, with a significant increase in the fecal score, gross lesion, and histopathological changes. Similarly, among the three weights, physical injury with a 100 g weight produced significant changes in the histopathological score in the model of AF. Intervention with HPLF-111624 at doses of 250 and 500 mg/kg b.wt., showed a reduction in the inflammatory cytokines and a significant improvement in the histopathological findings in both the conditions. CONCLUSION: The results showed that the modified experimental models for UP and AF resemble the human pathological conditions and are simple, versatile and may be used for screening drugs intended for these conditions. Intervention with HPLF-111624 was found to be effective in improving the pathological state of UP and AF. Dove 2022-04-27 /pmc/articles/PMC9058020/ /pubmed/35509969 http://dx.doi.org/10.2147/JEP.S345599 Text en © 2022 Onkaramurthy et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Onkaramurthy, Mallappa
Azeemuddin, Mohammed Mukhram
Baig, Mirza Rizwan
Kendaganna, Pavan Heggadadevanakote
Rafiq, Mohamed
Babu, Uddagiri Venkanna
Investigation of HPLF-111624 in Modified Experimental Models of Ulcerative Proctitis and Anal Fissure in Rats
title Investigation of HPLF-111624 in Modified Experimental Models of Ulcerative Proctitis and Anal Fissure in Rats
title_full Investigation of HPLF-111624 in Modified Experimental Models of Ulcerative Proctitis and Anal Fissure in Rats
title_fullStr Investigation of HPLF-111624 in Modified Experimental Models of Ulcerative Proctitis and Anal Fissure in Rats
title_full_unstemmed Investigation of HPLF-111624 in Modified Experimental Models of Ulcerative Proctitis and Anal Fissure in Rats
title_short Investigation of HPLF-111624 in Modified Experimental Models of Ulcerative Proctitis and Anal Fissure in Rats
title_sort investigation of hplf-111624 in modified experimental models of ulcerative proctitis and anal fissure in rats
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058020/
https://www.ncbi.nlm.nih.gov/pubmed/35509969
http://dx.doi.org/10.2147/JEP.S345599
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