Cargando…

Isobaric Tags for Relative and Absolute Quantitation Identification of Blood Proteins Relevant to Paroxetine Response in Patients With Major Depressive Disorder

OBJECTIVES: Isobaric tags for relative and absolute quantitation (iTRAQ) is a proteomic investigation that could be utilized for rapid identification and quantification of proteins, which we would use to identify differentially expressed proteins in treatment responsive patients with major depressiv...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Chin-Chuen, Su, Hung, Shiea, Jentaie, Huang, Tiao-Lai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058070/
https://www.ncbi.nlm.nih.gov/pubmed/35509884
http://dx.doi.org/10.3389/fpsyt.2022.577857
_version_ 1784698042087112704
author Lin, Chin-Chuen
Su, Hung
Shiea, Jentaie
Huang, Tiao-Lai
author_facet Lin, Chin-Chuen
Su, Hung
Shiea, Jentaie
Huang, Tiao-Lai
author_sort Lin, Chin-Chuen
collection PubMed
description OBJECTIVES: Isobaric tags for relative and absolute quantitation (iTRAQ) is a proteomic investigation that could be utilized for rapid identification and quantification of proteins, which we would use to identify differentially expressed proteins in treatment responsive patients with major depressive disorder (MDD). METHODS: Six treatment responsive patients of MDD were recruited, and their peripheral blood mononuclear cell (PBMC) were collected before and after 4 weeks of paroxetine treatment. iTRAQ and Mascot search engine were used to detect differentially expressed proteins, which were then validated by Western blot. RESULTS: Two thousand one hundred and fifty three proteins were screened, and seven proteins showed differences of more than two-fold and 62 proteins with a differences of less than two-fold. Six proteins with commercially available antibodies were identified, and were validated by Western blot in 10 paroxetine responsive MDD patients. Putative hydroxypyruvate isomerase (HYI), eukaryotic translation initiation factor 4H (eIF4H), and RNA binding motif 8A (RBM8A) had statistically significant differences before and after treatment in the validation. Data are available via ProteomeXchange with identifier PXD028947. CONCLUSIONS: By using iTRAQ and Western blot, we were able to identify HYI, eIF4H, and RAM8a to be the potential predictors of paroxetine treatment response in patients with MDD. This finding could help establish future individualized medicine.
format Online
Article
Text
id pubmed-9058070
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-90580702022-05-03 Isobaric Tags for Relative and Absolute Quantitation Identification of Blood Proteins Relevant to Paroxetine Response in Patients With Major Depressive Disorder Lin, Chin-Chuen Su, Hung Shiea, Jentaie Huang, Tiao-Lai Front Psychiatry Psychiatry OBJECTIVES: Isobaric tags for relative and absolute quantitation (iTRAQ) is a proteomic investigation that could be utilized for rapid identification and quantification of proteins, which we would use to identify differentially expressed proteins in treatment responsive patients with major depressive disorder (MDD). METHODS: Six treatment responsive patients of MDD were recruited, and their peripheral blood mononuclear cell (PBMC) were collected before and after 4 weeks of paroxetine treatment. iTRAQ and Mascot search engine were used to detect differentially expressed proteins, which were then validated by Western blot. RESULTS: Two thousand one hundred and fifty three proteins were screened, and seven proteins showed differences of more than two-fold and 62 proteins with a differences of less than two-fold. Six proteins with commercially available antibodies were identified, and were validated by Western blot in 10 paroxetine responsive MDD patients. Putative hydroxypyruvate isomerase (HYI), eukaryotic translation initiation factor 4H (eIF4H), and RNA binding motif 8A (RBM8A) had statistically significant differences before and after treatment in the validation. Data are available via ProteomeXchange with identifier PXD028947. CONCLUSIONS: By using iTRAQ and Western blot, we were able to identify HYI, eIF4H, and RAM8a to be the potential predictors of paroxetine treatment response in patients with MDD. This finding could help establish future individualized medicine. Frontiers Media S.A. 2022-04-18 /pmc/articles/PMC9058070/ /pubmed/35509884 http://dx.doi.org/10.3389/fpsyt.2022.577857 Text en Copyright © 2022 Lin, Su, Shiea and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Psychiatry
Lin, Chin-Chuen
Su, Hung
Shiea, Jentaie
Huang, Tiao-Lai
Isobaric Tags for Relative and Absolute Quantitation Identification of Blood Proteins Relevant to Paroxetine Response in Patients With Major Depressive Disorder
title Isobaric Tags for Relative and Absolute Quantitation Identification of Blood Proteins Relevant to Paroxetine Response in Patients With Major Depressive Disorder
title_full Isobaric Tags for Relative and Absolute Quantitation Identification of Blood Proteins Relevant to Paroxetine Response in Patients With Major Depressive Disorder
title_fullStr Isobaric Tags for Relative and Absolute Quantitation Identification of Blood Proteins Relevant to Paroxetine Response in Patients With Major Depressive Disorder
title_full_unstemmed Isobaric Tags for Relative and Absolute Quantitation Identification of Blood Proteins Relevant to Paroxetine Response in Patients With Major Depressive Disorder
title_short Isobaric Tags for Relative and Absolute Quantitation Identification of Blood Proteins Relevant to Paroxetine Response in Patients With Major Depressive Disorder
title_sort isobaric tags for relative and absolute quantitation identification of blood proteins relevant to paroxetine response in patients with major depressive disorder
topic Psychiatry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9058070/
https://www.ncbi.nlm.nih.gov/pubmed/35509884
http://dx.doi.org/10.3389/fpsyt.2022.577857
work_keys_str_mv AT linchinchuen isobarictagsforrelativeandabsolutequantitationidentificationofbloodproteinsrelevanttoparoxetineresponseinpatientswithmajordepressivedisorder
AT suhung isobarictagsforrelativeandabsolutequantitationidentificationofbloodproteinsrelevanttoparoxetineresponseinpatientswithmajordepressivedisorder
AT shieajentaie isobarictagsforrelativeandabsolutequantitationidentificationofbloodproteinsrelevanttoparoxetineresponseinpatientswithmajordepressivedisorder
AT huangtiaolai isobarictagsforrelativeandabsolutequantitationidentificationofbloodproteinsrelevanttoparoxetineresponseinpatientswithmajordepressivedisorder